LOCUS       sequence-32892-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Lowary PT, Widom J
  TITLE     New DNA sequence rules for high affinity binding to histone octamer 
            and sequence-directed nucleosome positioning.
  JOURNAL   J Mol Biol. 1998 Feb 13. 276(1):19-42.
  PUBMED    9514715
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            1998 Feb 13. 276(1):19-42."
COMMENT     Sequence Label: pGEM-3z/603
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(365..383)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(366..383)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(401..417)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(401..417)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(414..436)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    425..441
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(449..455,456..473,474..479))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    494..515
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(632..649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(883..902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tataaagact cctatgggcg aattcgagct cggtacccgg ggatcctcta gagtcgggag
       61 ctcggnacac tatccgactg gcaccgaaac gggtacccca gggacttgaa gtaataagga
      121 cggagggcct ctttcaacat cgatgcacgg tggttagcct tggattgcgc tctaccgtgc
      181 gctaagcgta cttagaagcc cgagtgacga cttcacacgg taggtgggcg cgcgaactgg
      241 gcacccgaga gtgtcgatta ttttacggct cacgctgggg tgatttgtac taggaaaacg
      301 cctattcgtg tattccgcct tggtcattag gatcccggac ctgcaggcat gcaagcttga
      361 gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      421 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      481 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      541 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      601 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      661 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      781 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      841 atcgacgctc aagtcagaag tggcgaaacc cgacaggact ataaagatac caggcgtttc
      901 cccctgaact cctc
//
