LOCUS       sequence-32893-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Lowary PT, Widom J
  TITLE     New DNA sequence rules for high affinity binding to histone octamer 
            and sequence-directed nucleosome positioning.
  JOURNAL   J Mol Biol. 1998 Feb 13. 276(1):19-42.
  PUBMED    9514715
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            1998 Feb 13. 276(1):19-42."
COMMENT     Sequence Label: pGEM-3z/605
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(370..388)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(371..388)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(406..422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(406..422)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(419..441)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    430..446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(454..460,461..478,479..484))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    499..520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(637..654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(888..907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcacgatgaa gactcactat agggcgaatt cgagctcggt acccggggat cctctagagt
       61 gggagctcgg aacactatcc gactggcacc cggcgtgtcc tattaatatg gtcgtgacgg
      121 tggataccgg ctcaataggt gattagcgtc gagcttgaat acccttgggc ggctaaaacg
      181 acggggctag ggttgtaacg tcgtttaagc gtatctagac cggtctcgca ctcaatgtgc
      241 gataggcatc cccatatccc gcgtcgtccg aaccttgacc tgtcgctatc gccatctaga
      301 gcatctgtca caccaaccag taccttggtc attaggatcc cggacctgca ggcatgcaag
      361 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc
      421 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      481 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      541 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      601 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      661 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      721 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      781 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      841 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      901 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      961 cctgtccgcc tttctccctt cggga
//
