LOCUS       sequence-328936-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Elias RD, Ramaraju B, Deshmukh L
  TITLE     Mechanistic Roles of Tyrosine Phosphorylation in Reversible 
            Amyloids, Autoinhibition, and Endosomal Membrane Association of 
            ALIX.
  JOURNAL   J Biol Chem. 2021 Oct 21:101328. doi: 10.1016/j.jbc.2021.101328.
  PUBMED    34688656
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2021 Oct 21:101328. doi: 10.1016/j.jbc.2021.101328."
COMMENT     Sequence Label:  GB1-PRD(703-868, P801G, G808C, C813S)-Strep
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             169..186
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             187..198
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             238..255
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             256..276
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             775..798
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atgcaataca aactggcgct gaacggcaaa acgctgaagg gtgaaacgac gaccgaggcc
       61 gttgatgcgg ccacggccga gaaagtgttc aagcagtacg ccaacgacaa cggtgttgat
      121 ggcgaatgga cctacgacga tgcgaccaaa accttcaccg tgaccgaact ggttccgcgc
      181 ggcagtatcg aaggtcgcat ccgcgagaat ctgaaagata gcggtctgtt tctggagcat
      241 caccaccatc accatgaaaa tctgtacttc caaggcgacg aactgctgaa agatctgcag
      301 cagagcattg cccgcgaacc aagcgcccca agcatcccga cgccagccta tcagagtagt
      361 ccggcgggtg gtcatgcgcc gaccccacca acgccggccc cacgtacgat gccgccaacc
      421 aaaccgcaac caccagcgcg tccgccacca ccagttctcc cagccaatcg cgccccaagt
      481 gcgaccgcgc caagcccagt tggtgcgggt accgccgcgc cagcgccaag tcaaacgccg
      541 ggtagtgccc caccaccaca agcgcaaggc ggcccatatc cgacgtaccc gtgttatccg
      601 ggctacagtc agatgccaat gccgatgggt tacaacccat acgcgtacgg ccagtataac
      661 atgccgtacc caccggtgta ccaccagagt ccgggccaag ccccataccc gggtccacag
      721 cagccaagct acccgttccc gcagccacca cagcaaagtt actacccgca gcaatggagc
      781 catccgcagt tcgaaaagta a
//
