LOCUS       sequence-328948-        2086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express IKK-epsilon v2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Chang YL, Liao YW, Chen MH, Chang SY, Huang YT, Ho BC, Yu SL
  TITLE     IKKepsilon isoform switching governs the immune response against 
            EV71 infection.
  JOURNAL   Commun Biol. 2021 Jun 2;4(1):663. doi: 10.1038/s42003-021-02187-x.
  PUBMED    34079066
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-06-2"; volume: "4(1):663. doi"; pages: " 
            10.1038/s42003-021-02187-x"
COMMENT     Sequence Label: F-IKK-epsilon v2
FEATURES             Location/Qualifiers
     source          1..2086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10..33
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2031..2047)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2032..2048)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gccaccatgg attacaagga tgacgacgat aagagcccgg gcggatccat gcagagcaca
       61 gccaattacc tgtggcacac agatgacctg ctggggcagg gggccactgc cagtgtgtac
      121 aaggcccgca acaagaaatc cggagagctg gttgctgtga aggtcttcaa cactaccagc
      181 tacctgcggc cccgcgaggt gcaggtgagg gagtttgagg tcctgcggaa gctgaaccac
      241 cagaacatcg tcaagctctt tgcggtggag gagacgggcg gaagccggca gaaggtactg
      301 gtgatggagt actgctccag tgggagcctg ctgagtgtgc tggagagccc tgagaatgcc
      361 tttgggctgc ctgaggatga gttcctggtg gtgctgcgct gtgtggtggc cagcatgaac
      421 cacctgcggg agaacggcat tgtgcatcgc gacatcaagc cggggaacat catgcgcctc
      481 gtaggggagg aggggcagag catctacaag ctgacagact tcggcgctgc ccgggagctg
      541 gatgatgatg agaagttcgt ctcggtctat gggactgagg agtacctgca tcccgacatg
      601 tatgagcggg cggtgcttcg aaagccccag caaaaagcgt tcggggtgac tgtggatctc
      661 tggagcattg gagtgacctt gtaccatgca gccactggca gcctgccctt catccccttt
      721 ggtgggccac ggcggaacaa ggagatcatg taccggatca ccacggagaa gccggctggg
      781 gccattgcag gtgcccagag gcgggagaac gggcccctgg agtggagcta caccctcccc
      841 atcacctgcc agctgtcact ggggctgcag agccagctgg tgcccatcct ggccaacatc
      901 ctggaggtgg agcaggccaa gtgctggggc ttcggccagt tctttgcgga gaccagtgac
      961 atcctgcagc gagttgtcgt ccatgtcttc tccctgtccc aggcagtcct gcaccacatc
     1021 tatatccatg cccacaacac gatagccatt ttccaggagg ccgtgcacaa gcagaccagt
     1081 gtggcccccc gacaccagga gtacctcttt gagggtcacc tctgtgtcct cgagcccagc
     1141 gtctcagcac agcacatcgc ccacacgacg gcaagcagcc ccctgaccct cttcagcaca
     1201 gccatcccta aggggctggc cttcagggac cctgctctgg acgtccccaa gttcgtcccc
     1261 aaagtggacc tgcaggcgga ttacaacact gccaagggcg tgttgggcgc cggctaccag
     1321 gccctgcggc tggcacgggc cctgctggat gggcaggagc taatgtttcg ggggctgcac
     1381 tgggtcatgg aggtgctcca ggccacatgc agacggactc tggaagtggc aaggacatcc
     1441 ctcctctacc tcagcagcag cctgggaact gagaggttca gcagcgtggc tggaacgcct
     1501 gagatccagg aactgaaggc ggctgcagaa ctgaggtcca ggctgcggac tctagcggag
     1561 gtcctctcca gatgctccca aaatatcacg gagacccagg agagcctgag cagcctgaac
     1621 cgggagctgg tgaagagccg ggatcaggta catgaggaca gaagcatcca gcagattcag
     1681 tgctgtttgg acaagatgaa cttcatctac aaacagttca agaagtctag gatgaggcca
     1741 gggcttggct acaacgagga gcagattcac aagctggata aggtgaattt cagtcattta
     1801 gccaaaagac tcctgcaggt gttccaggag gagtgcgtgc agaagtatca agcgtcctta
     1861 gtcacacacg gcaagaggat gagggtggtg cacgagacca ggaaccacct gcgcctggtt
     1921 ggctgttctg tggctgcctg taacacagaa gcccaggggg tccaggagag tctcagcaag
     1981 catgcaagag ctctgcgagg ggatgaagct gctggcatct gaaagcttat cgataccgtc
     2041 gacctcgagg gggggcccgg taccttaata ataagtacca gtaacc
//
