LOCUS       sequence-328952-        1560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express IRF7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Chang YL, Liao YW, Chen MH, Chang SY, Huang YT, Ho BC, Yu SL
  TITLE     IKKepsilon isoform switching governs the immune response against 
            EV71 infection.
  JOURNAL   Commun Biol. 2021 Jun 2;4(1):663. doi: 10.1038/s42003-021-02187-x.
  PUBMED    34079066
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-06-2"; volume: "4(1):663. doi"; pages: " 
            10.1038/s42003-021-02187-x"
COMMENT     Sequence Label: F-IRF7
FEATURES             Location/Qualifiers
     source          1..1560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10..33
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gccaccatgg attacaagga tgacgacgat aagagcccgg gcggatccat ggccttggct
       61 cctgagaggg cagccccacg cgtgctgttc ggagagtggc tccttggaga gatcagcagc
      121 ggctgctatg aggggctgca gtggctggac gaggcccgca cctgtttccg cgtgccctgg
      181 aagcacttcg cgcgcaagga cctgagcgag gccgacgcgc gcatcttcaa ggcctgggct
      241 gtggcccgcg gcaggtggcc gcctagcagc aggggaggtg gcccgccccc cgaggctgag
      301 actgcggagc gcgccggctg gaaaaccaac ttccgctgcg cactgcgcag cacgcgtcgc
      361 ttcgtgatgc tgcgggataa ctcgggggac ccggccgacc cgcacaaggt gtacgcgctc
      421 agccgggagc tgtgctggcg agaaggccca ggcacggacc agactgaggc agaggccccc
      481 gcagctgtcc caccaccaca gggtgggccc ccagggccat tcctggcaca cacacatgct
      541 ggactccaag ccccaggccc cctccctgcc ccagctggtg acaaggggga cctcctgctc
      601 caggcagtgc aacagagctg cctggcagac catctgctga cagcgtcatg gggggcagat
      661 ccagtcccaa ccaaggctcc tggagaggga caagaagggc ttcccctgac tggggcctgt
      721 gctggaggcc cagggctccc tgctggggag ctgtacgggt gggcagtaga gacgaccccc
      781 agccccgggc cccagcccgc ggcactaacg acaggcgagg ccgcggcccc agagtccccg
      841 caccaggcag agccgtacct gtcaccctcc ccaagcgcct gcaccgcggt gcaagagccc
      901 agcccagggg cgctggacgt gaccatcatg tacaagggcc gcacggtgct gcagaaggtg
      961 gtgggacacc cgagctgcac gttcctatac ggccccccag acccagctgt ccgggccaca
     1021 gacccccagc aggtagcatt ccccagccct gccgagctcc cggaccagaa gcagctgcgc
     1081 tacacggagg aactgctgcg gcacgtggcc cctgggttgc acctggagct tcgggggcca
     1141 cagctgtggg cccggcgcat gggcaagtgc aaggtgtact gggaggtggg cggaccccca
     1201 ggctccgcca gcccctccac cccagcctgc ctgctgcctc ggaactgtga cacccccatc
     1261 ttcgacttca gagtcttctt ccaagagctg gtggaattcc gggcacggca gcgccgtggc
     1321 tccccacgct ataccatcta cctgggcttc gggcaggacc tgtcagctgg gaggcccaag
     1381 gagaagagcc tggtcctggt gaagctggaa ccctggctgt gccgagtgca cctagagggc
     1441 acgcagcgtg agggtgtgtc ttccctggat agcagcagcc tcagcctctg cctgtccagc
     1501 gccaacagcc tctatgacga catcgagtgc ttccttatgg agctggagca gcccgcctag
//
