LOCUS       sequence-328958-        1659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express IRF7 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Chang YL, Liao YW, Chen MH, Chang SY, Huang YT, Ho BC, Yu SL
  TITLE     IKKepsilon isoform switching governs the immune response against 
            EV71 infection.
  JOURNAL   Commun Biol. 2021 Jun 2;4(1):663. doi: 10.1038/s42003-021-02187-x.
  PUBMED    34079066
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-06-2"; volume: "4(1):663. doi"; pages: " 
            10.1038/s42003-021-02187-x"
COMMENT     Sequence Label: H-IRF7
FEATURES             Location/Qualifiers
     source          1..1659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..45
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             52..96
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             112..126
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 caccatcatc atcatcattc ttctggtctg gtgccacgcg gttctggtat gaaagaaacc
       61 gctgctgcta aattcgaacg ccagcacatg gacagcccag atctgggtac cgacgacgac
      121 gacaaggcca tggctgatat cggatccatg gccttggctc ctgagagggc agccccacgc
      181 gtgctgttcg gagagtggct ccttggagag atcagcagcg gctgctatga ggggctgcag
      241 tggctggacg aggcccgcac ctgtttccgc gtgccctgga agcacttcgc gcgcaaggac
      301 ctgagcgagg ccgacgcgcg catcttcaag gcctgggctg tggcccgcgg caggtggccg
      361 cctagcagca ggggaggtgg cccgcccccc gaggctgaga ctgcggagcg cgccggctgg
      421 aaaaccaact tccgctgcgc actgcgcagc acgcgtcgct tcgtgatgct gcgggataac
      481 tcgggggacc cggccgaccc gcacaaggtg tacgcgctca gccgggagct gtgctggcga
      541 gaaggcccag gcacggacca gactgaggca gaggcccccg cagctgtccc accaccacag
      601 ggtgggcccc cagggccatt cctggcacac acacatgctg gactccaagc cccaggcccc
      661 ctccctgccc cagctggtga caagggggac ctcctgctcc aggcagtgca acagagctgc
      721 ctggcagacc atctgctgac agcgtcatgg ggggcagatc cagtcccaac caaggctcct
      781 ggagagggac aagaagggct tcccctgact ggggcctgtg ctggaggccc agggctccct
      841 gctggggagc tgtacgggtg ggcagtagag acgaccccca gccccgggcc ccagcccgcg
      901 gcactaacga caggcgaggc cgcggcccca gagtccccgc accaggcaga gccgtacctg
      961 tcaccctccc caagcgcctg caccgcggtg caagagccca gcccaggggc gctggacgtg
     1021 accatcatgt acaagggccg cacggtgctg cagaaggtgg tgggacaccc gagctgcacg
     1081 ttcctatacg gccccccaga cccagctgtc cgggccacag acccccagca ggtagcattc
     1141 cccagccctg ccgagctccc ggaccagaag cagctgcgct acacggagga actgctgcgg
     1201 cacgtggccc ctgggttgca cctggagctt cgggggccac agctgtgggc ccggcgcatg
     1261 ggcaagtgca aggtgtactg ggaggtgggc ggacccccag gctccgccag cccctccacc
     1321 ccagcctgcc tgctgcctcg gaactgtgac acccccatct tcgacttcag agtcttcttc
     1381 caagagctgg tggaattccg ggcacggcag cgccgtggct ccccacgcta taccatctac
     1441 ctgggcttcg ggcaggacct gtcagctggg aggcccaagg agaagagcct ggtcctggtg
     1501 aagctggaac cctggctgtg ccgagtgcac ctagagggca cgcagcgtga gggtgtgtct
     1561 tccctggata gcagcagcct cagcctctgc ctgtccagcg ccaacagcct ctatgacgac
     1621 atcgagtgct tccttatgga gctggagcag cccgcctag
//
