LOCUS       sequence-32897-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Lowary PT, Widom J
  TITLE     New DNA sequence rules for high affinity binding to histone octamer 
            and sequence-directed nucleosome positioning.
  JOURNAL   J Mol Biol. 1998 Feb 13. 276(1):19-42.
  PUBMED    9514715
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            1998 Feb 13. 276(1):19-42."
COMMENT     Sequence Label: pGEM-3z/618
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(353..371)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(354..371)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(389..405)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(389..405)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(402..424)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    413..429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(437..443,444..461,462..467))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    482..503
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(620..637)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatgggcga attcgagctc ggtacccggg gatcctctag agtcgggagc tcggaacact
       61 atccgactgg caccatagtc aggaacgcga atgaggatcg ctttgaaata aatatggctc
      121 gttttcgcgc atgccacgtt ctcaaccctt ggatggagcc gaagcagtac gagcagagcc
      181 aaacaaggga tcaccagggg ctcgctcgca gttgagggcc cgaacccagg cacccgtgtt
      241 gtgaaccgct cagaatcgac agtagcgcac gctaagcggg tctagagatg tacaacgatc
      301 tatgtacctt ggtcattagg atcccgacct gcaggcatgc aagcttgagt attctatagt
      361 gtcacctaaa tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      421 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      481 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      541 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      601 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      661 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      721 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      781 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      841 gtcaga
//
