LOCUS       sequence-328982-        4317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri ADCK3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-ADCK3(1of3)
FEATURES             Location/Qualifiers
     source          1..4317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2442..2541)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2558..2577)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2559..2577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2582..2598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2582..2598)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2700..2719)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2711..3520
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2778..2797)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3667..4255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4156..4175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgac aggtgactct gtggtctgga gaggtctgag
      721 atgggtgaag gcagccagca gagccccaac aagcagtctg ctacaccttc acccccctgg
      781 atcagcaagg atggagcatt gcctccacag actcgcatca gcgcagcttc acagatactc
      841 aaccgtgagc gcttgcttca gctccggctt ctctggaggt ttaaacggca gtctggggac
      901 gaccggcaga gggtttggtg ctaaacatgt ggagtaccac aggaagtggc cgtgccagct
      961 gttttctggt cagacgcgcc agtttaacca ggacggctcc acagtgggcc ggctgacgtc
     1021 agacgacatc cagaaagcca gaaaagccaa gaaaactgaa gccaaggaat ataaacaatc
     1081 gctcagcgac cgttccagag agaggaaggt gcccgtcaca cggataggac ggctggtgaa
     1141 ttttggaggc ctggcatttg gacttggact tggagcgctt gcagaggtgg cgaagaacag
     1201 tttcaaaccc aaacaacaag ttttttcagg aagcaaaaag gccgttctgg actccaatcc
     1261 tttcctgtct gaagctaacg ctgaaaggat cgtccgaacc ctctgcaaag tccggggggc
     1321 cgctctgaaa ataggacaga tgctcagtat tcaggacgac gccttcatga accctcagct
     1381 ggctaaaatc ttcgagcgcg ttcgtcagag tgctgacttc atgccctcta agcagatgat
     1441 gaaaatcatc aacaacgatc tgggtccgaa ctggagggac cagctggagt actttgagga
     1501 gaagccgttt gcagctgcct ccatcggtca ggtgcactta gggaggttga aggatggcag
     1561 agaagtggcc atgaagatcc agtaccctgg aattgccaaa agcatcaaca gtgacgttcg
     1621 taacatcatg acggctctga gtttaagcaa cgctctgcct caaggtttgt tccccgagca
     1681 tctgatcgag gtcatgagtc atgaactggc cctggagtgt gattacataa gagaggctaa
     1741 atgtgcccga aagtttcaag agctgctgaa ggaccaccct ttcttctacg tgcctgacgt
     1801 gatcgacgag ctgagcagcg agcgcgtctt caccacaacc ctggtgtctg gtttccccct
     1861 ggaccgggcc acggatctcc cccaggagct caggaatgag atctgcgagc agatcctaat
     1921 tttgtgcctg agagaacttt tcgagttcag attcatgcag acggatccaa actggtccaa
     1981 cttcttctat gatccacaac ttcacaaggt tgccctgttg gactttggag ccacgcgaga
     2041 gtttgacaag agtttcactg acgcctacat cgaggtcatc aaggcggctg cagatcagga
     2101 ccgagaaaaa gtcctgctca gatccagaga catgaagttc ctcacgggat tcgagtcaaa
     2161 ggcgatggag aacgcccacg tggaggcggt gatgatcctc ggagaggcct tctcctcaga
     2221 aaaagccttc gacttcggag agcagagcac caccgagcgc atccaccagc tgatcccggt
     2281 gatgctgagg gagcggctga cgcccccgcc ggaggaaacc tactccctcc acaggaagat
     2341 gggcggctcc ttcctcatct gctccaaact aaaagctaaa atctgctgta agcacatgtt
     2401 ccaggaggcc tacaggaact accagagagg cctggacccc gacccagctt tcttgtacaa
     2461 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     2521 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
     2581 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     2641 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     2701 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
     2761 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     2821 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     2881 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     2941 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     3001 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     3061 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     3121 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     3181 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     3241 gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt
     3301 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     3361 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     3421 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     3481 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     3541 gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc
     3601 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3661 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3721 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3781 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3841 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3901 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3961 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4021 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4081 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4141 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4201 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4261 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
