LOCUS       sequence-32899-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Lowary PT, Widom J
  TITLE     New DNA sequence rules for high affinity binding to histone octamer 
            and sequence-directed nucleosome positioning.
  JOURNAL   J Mol Biol. 1998 Feb 13. 276(1):19-42.
  PUBMED    9514715
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            1998 Feb 13. 276(1):19-42."
COMMENT     Sequence Label: pGEM-3z/626
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(354..372)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(355..372)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(390..406)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(390..406)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(403..425)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    414..430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(438..444,445..462,463..468))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    483..504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(621..638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatgggcga attcgagctc ggtacccggg gatcctctag agtcgggagc tcgncacttt
       61 atccgactgg cacccacccg acactaaaaa gcacgtcccg aaagggcacc ggataatgcc
      121 acaggtggac gatttatagg cactatcctt ggagatgcag gagaggactc taatagtgct
      181 tagcgctacg tagacgccat tccagagtca agcggcgata caatacccgg caccggtcga
      241 ctctcatcga atcgcagagc gacaaggctt ggtggagctt aactatctcg aaattccctc
      301 caatcacctt ggtcattagg atcccggacc tgcaggcatg caagcttgag tattctatag
      361 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
      421 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      481 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      541 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      601 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      661 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgttttc cccctggaag
      901 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcc
//
