LOCUS       sequence-328990-        4073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri ATP6V1B2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-ATP6V1B2
FEATURES             Location/Qualifiers
     source          1..4073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2198..2297)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2314..2333)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2315..2333)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2338..2354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2338..2354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2456..2475)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2467..3276
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2534..2553)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3423..4011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3912..3931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgat ggcaacactg gtggcaaaca gaaatgtgga
      721 tatcaacggt ttggcagccg ctgcgaggac acacactcaa gctgtgacca ggaattacat
      781 atctcagccc aggctaacat actccaccgt gtcgggagta aacgggcccc tggtgatctt
      841 ggataacgta aagtttccca gatatgctga gattgttcat ctgactctgc cagacggcac
      901 caggaggagt gggcaggtcc tggaggtgat tggcagcaaa gcagtcgtcc aggtgtttga
      961 gggaacgtcg ggcattgatg ccaagaagac cgcctgtgag tttacgggtg acatcctgcg
     1021 tacgccggtg tcggaggaca tgttgggtcg agtcttcaac ggttctggga agcccatcga
     1081 ccgtgggccc tgtgtcctgg ctgaggacta cttggacatc atgggtcagc caatcaatcc
     1141 tcagtgccgc atctaccctg aggagatgat ccagactggc atctcggcca tcgacggcat
     1201 gaacagcatc gccagaggac agaagatccc catcttctct gctgctggtt taccccacaa
     1261 cgagatcgct gctcagatct gccgccaggc cggcctcgtg cagaaatcca aggacgtgat
     1321 ggactacagc gccgagaact tcgccatcgt ctttgctgcc atgggagtca acatggaaac
     1381 ggctcgcttc tttaaatccg actttgagga aaacggctcc atggacaacg tctgcctttt
     1441 cctgaatctg gccaacgacc ccaccatcga gcgcatcatt acgccccgcc tggccctgac
     1501 gacagctgag tacctggcct accagtgtga gaaacacgtg ctggtcatcc tgacggacat
     1561 gagctcctac gccgaagctc tgagagaggt ctctgctgcc cgggaggaag tacccggccg
     1621 tagaggtttt ccaggttaca tgtacaccga tctggccacc atctacgagc gtgcgggccg
     1681 agtggagggc agaaacggct ccatcaccca gatccccatc ctcaccatgc ccaacgatga
     1741 tatcactcat cccattcccg atctcactgg gtacatcaca gagggtcagg tctacgttga
     1801 ccgacagctg cacaacagac agatttaccc ccccattaac gtgctgcctt ccctgtctcg
     1861 tctgatgaag tctgccatcg gtgaagggat gaccaggaag gaccacgccg atgtgtccaa
     1921 ccagctgtac gcctgctacg ccattggtaa ggacgtccag gccatgaagg ctgtggttgg
     1981 agaagaggcc ctgacctcag atgacctgct ctacctggag ttcctgcaga agtttgagaa
     2041 gaattttatt gcccagggtc cctatgacaa caggaccgtc tatgaaaccc tggacatcgg
     2101 atggcagcta ctgcgaatct tccccaagga gatgctcaag aggattcctc agagcactct
     2161 ggctgagttc taccccaggg attcctccgc ccgccagacc cagctttctt gtacaaagtt
     2221 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     2281 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     2341 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     2401 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     2461 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     2521 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     2581 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     2641 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     2701 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2761 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2821 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2881 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2941 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     3001 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     3061 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     3121 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     3181 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3241 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3301 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3361 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3421 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3481 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3541 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3601 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3661 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3721 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3781 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3841 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3901 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3961 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4021 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtt
//
