LOCUS       sequence-328996-        6678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged CAD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD
FEATURES             Location/Qualifiers
     source          1..6678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcggccc tagtgttgga ggacgggtcg gtcctgcggg gccagccctt tggggccgcc
       61 gtgtcgactg ccggggaagt ggtgtttcaa accggcatgg tcggctaccc cgaggccctc
      121 actgatccct cctacaaggc acagatctta gtgctcacct atcctctgat cggcaactat
      181 ggcatccccc cagatgaaat ggatgagttc ggtctctgca agtggtttga atcctcgggc
      241 atccacgtag cagcactggt agtgggagag tgctgtccta ctcccagcca ctggagtgcc
      301 acccgcaccc tgcatgagtg gctgcagcag catggcatcc ctggcttgca aggagtagac
      361 actcgggagc tgaccaagaa gttgcgggaa caggggtctc tgctggggaa gctggtccag
      421 aatggaacag aaccttcatc cctgccattc ttggacccca atgcccgccc cctggtacca
      481 gaggtctcca ttaagactcc acgggtattc aatacagggg gtgcccctcg gatccttgct
      541 ttggactgtg gcctcaagta taatcagatc cgatgcctct gccagcgtgg ggctgaggtc
      601 actgtggtac cctgggacca tgcactagac agccaagagt atgagggtct cttcttaagt
      661 aatgggcctg gtgaccctgc ctcctatccc agtgtcgtat ccacactgag ccgtgtttta
      721 tctgagccta atccccgacc tgtctttggg atctgcctgg gacaccagct attggcctta
      781 gccattgggg ccaagactta caagatgaga tatgggaacc gaggccataa ccagccctgc
      841 ttgttggtgg gctctgggcg ctgctttctg acatcccaga accatgggtt tgctgtggag
      901 acagactcac tgccagcaga ctgggctcct ctcttcacca acgccaatga tggttccaat
      961 gaaggcattg tgcacaacag cttgcctttc ttcagtgtcc agtttcaccc agagcaccaa
     1021 gctggccctt cagatatgga actgcttttc gatatctttc tggaaactgt gaaagaggcc
     1081 acagctggga accctggggg ccagacagtt agagagcggc tgactgagcg cctctgtccc
     1141 cctgggattc ccactcccgg ctctggactt ccaccaccac gaaaggttct gatcctgggc
     1201 tcagggggcc tctccattgg ccaagctgga gaatttgact actcgggctc tcaggcaatt
     1261 aaggccctga aggaggaaaa catccagacg ttgctgatca accccaatat tgccacagtg
     1321 cagacctccc aggggctggc cgacaaggtc tattttcttc ccataacacc tcattatgta
     1381 acccaggtga tacgtaatga acgccccgat ggtgtgttac tgacttttgg gggccagact
     1441 gctctgaact gtggtgtgga gctgaccaag gccggggtgc tggctcggta tggggtccgg
     1501 gtcctgggca caccagtgga gaccattgag ctgaccgagg atcgacgggc ctttgctgcc
     1561 agaatggcag agatcggaga gcatgtggcc ccgagcgagg cagcaaattc tcttgaacag
     1621 gcccaggcag ccgctgaacg gctggggtac cctgtgctag tgcgtgcagc ctttgccctg
     1681 ggtggcctgg gctctggctt tgcctctaac agggaggagc tctctgctct cgtggcccca
     1741 gcttttgccc ataccagcca agtgctagta gacaagtctc tgaagggatg gaaggagatt
     1801 gagtacgagg tggtgagaga cgcctatggc aactgtgtca cggtgtgtaa catggagaac
     1861 ttggacccac tgggcatcca cactggtgag tccatagtgg tggcccctag ccagacactg
     1921 aatgacaggg agtatcagct cctgaggcag acagctatca aggtgaccca gcacctggga
     1981 attgttgggg agtgcaatgt gcagtatgcc ttgaaccctg agtctgagca gtattacatc
     2041 attgaagtga atgccaggct ctctcgcagc tctgccctgg ccagtaaggc cacaggttat
     2101 ccactggctt atgtggcagc caagctagca ttgggcatcc ctttgcctga gctcaggaac
     2161 tctgtgacag ggggtacagc agcctttgaa cccagcgtgg attattgtgt ggtgaagatt
     2221 cctcgatggg accttagcaa gttcctgcga gtcagcacaa agattgggag ctgcatgaag
     2281 agcgttggtg aagtcatggg cattgggcgt tcatttgagg aggccttcca gaaggccctg
     2341 cgcatggtgg atgagaactg tgtgggcttt gatcacacag tgaaaccagt cagcgatatg
     2401 gagttggaga ctccaacaga taagcggatt tttgtggtgg cagctgcttt gtgggctggt
     2461 tattcagtgg accgcctgta tgagctcaca cgcatcgacc gctggttcct gcaccgaatg
     2521 aagcgtatca tcgcacatgc ccagctgcta gaacaacacc gtggacagcc tttgccgcca
     2581 gacctgctgc aacaggccaa gtgtcttggc ttctcagaca aacagattgc ccttgcagtt
     2641 ctgagcacag agctggctgt tcgcaagctg cgtcaggaac tggggatctg tccagcagtg
     2701 aaacagattg acacagttgc agctgagtgg ccagcccaga caaattacct atacctaacg
     2761 tattggggca ccacccatga cctcaccttt cgaacacctc atgtcctagt ccttggctct
     2821 ggcgtctacc gtattggctc tagcgttgaa tttgactggt gtgctgtagg ctgcatccag
     2881 cagctccgaa agatgggata taagaccatc atggtgaact ataacccaga gacagtcagc
     2941 accgactatg acatgtgtga tcgactctac tttgatgaga tctcttttga ggtggtgatg
     3001 gacatctatg agctcgagaa ccctgaaggt gtgatcctat ccatgggtgg acagctgccc
     3061 aacaacatgg ccatggcgtt gcatcggcag cagtgccggg tgctgggcac ctcccctgaa
     3121 gccattgact cggctgagaa ccgtttcaag ttttcccggc tccttgacac cattggtatc
     3181 agccagcctc agtggaggga gctcagtgac ctcgagtctg ctcgccaatt ctgccagacc
     3241 gtggggtacc cctgtgtggt gcgcccctcc tatgtgctga gcggtgctgc tatgaatgtg
     3301 gcctacacgg atggagacct ggagcgcttc ctgagcagcg cagcagccgt ctccaaagag
     3361 catcccgtgg tcatctccaa gttcatccag gaggctaagg agattgacgt ggatgccgtg
     3421 gcctctgatg gtgtggtggc agccatcgcc atctctgagc atgtggagaa tgcaggtgtg
     3481 cattcaggtg atgcgacgct ggtgaccccc ccacaagata tcactgccaa aaccctggag
     3541 cggatcaaag ccattgtgca tgctgtgggc caggagctac aggtcacagg acccttcaat
     3601 ctgcagctca ttgccaagga tgaccagctg aaagttattg aatgcaacgt acgtgtctct
     3661 cgctccttcc ccttcgtttc caagacactg ggtgtggacc tagtagcctt ggccacgcgg
     3721 gtcatcatgg gggaagaagt ggaacctgtg gggctaatga ctggttctgg agttgtggga
     3781 gtaaaggtgc ctcagttctc cttctcccgc ttggcgggtg ctgacgtggt gttgggtgtg
     3841 gaaatgacca gtactgggga ggtggccggc tttggggaga gccgctgtga ggcatacctc
     3901 aaggccatgc taagcactgg ctttaagatc cccaagaaga atatcctgct gaccattggc
     3961 agctataaga acaaaagcga gctgctccca actgtgcggc tactggagag cctgggctac
     4021 agcctctatg ccagtctcgg cacagctgac ttctacactg agcatggcgt caaggtaaca
     4081 gctgtggact ggcactttga ggaggctgtg gatggtgagt gcccaccaca gcggagcatc
     4141 ctggagcagc tagctgagaa aaactttgag ctggtgatta acctgtcaat gcgtggagct
     4201 gggggccggc gtctctcttc ctttgtcacc aagggctacc gcacccgacg cttggccgct
     4261 gacttctccg tgcccctaat catcgatatc aagtgcacca aactctttgt ggaggcccta
     4321 ggccagatcg ggccagcccc tcctttgaag gtgcatgttg actgtatgac ctcccaaaag
     4381 cttgtgcgac tgccgggatt gattgatgtc catgtgcacc tgcgggaacc aggtgggaca
     4441 cataaggagg actttgcttc aggcacagcc gctgccctgg ctgggggtat caccatggtg
     4501 tgtgccatgc ctaatacccg gccccccatc attgacgccc ctgctctggc cctggcccag
     4561 aagctggcag aggctggcgc ccggtgcgac tttgcgctat tccttggggc ctcgtctgaa
     4621 aatgcaggaa ccttgggcac cgtggccggg tctgcagccg ggctgaagct ttacctcaat
     4681 gagaccttct ctgagctgcg gctggacagc gtggtccagt ggatggagca tttcgagaca
     4741 tggccctccc acctccccat tgtggctcac gcggagcagc aaaccgtggc tgctgtcctc
     4801 atggtggctc agctcactca gcgctcagtg cacatatgtc acgtggcacg gaaggaggag
     4861 atcctgctaa ttaaagctgc aaaggcacgg ggcttgccag tgacctgcga ggtggctccc
     4921 caccacctgt tcctaagcca tgatgacctg gagcgcctgg ggcctgggaa gggggaggtc
     4981 cggcctgagc ttggctcccg ccaggatgtg gaagccctgt gggagaacat ggctgtcatc
     5041 gactgctttg cctcagacca tgctccccat accttggagg agaagtgtgg gtccaggccc
     5101 ccacctgggt tcccagggtt agagaccatg ctgccactac tcctgacggc tgtaagcgag
     5161 ggccggctca gcctggacga cctgctgcag cgattgcacc acaatcctcg gcgcatcttt
     5221 cacctgcctc cgcaggagga cacctatgtg gaggtggatc tggagcatga gtggacaatt
     5281 cccagccaca tgcccttctc caaggcccac tggacacctt ttgaagggca gaaagtgaag
     5341 ggcaccgtcc gccgtgtggt cctgcgaggg gaggttgcct atatcgatgg gcaggttctg
     5401 gtacccccgg gctatggaca ggatgtacgg aagtggccac agggggctgt tcctcagctc
     5461 ccaccctcag cccctgccac tagtgagatg accacgacac ctgaaagacc ccgccgtggc
     5521 atcccagggc ttcctgatgg ccgcttccat ctgccgcccc gaatccatcg agcctccgac
     5581 ccaggtttgc cagctgagga gccaaaggag aagtcctctc ggaaggtagc cgagccagag
     5641 ctgatgggaa cccctgatgg cacctgctac cctccaccac cagtaccgag acaggcatct
     5701 ccccagaacc tggggacccc tggcttgctg cacccccaga cctcacccct gctgcactca
     5761 ttagtgggcc aacatatcct gtccgtccag cagttcacca aggatcagat gtctcacctg
     5821 ttcaatgtgg cacacacact gcgtatgatg gtgcagaagg agcggagcct cgacatcctg
     5881 aaggggaagg tcatggcctc catgttctat gaagtgagca cacggaccag cagctccttt
     5941 gcagcagcca tggcccggct gggaggtgct gtgctcagct tctcggaagc cacatcgtcc
     6001 gtccagaagg gcgaatccct ggctgactcc gtgcagacca tgagctgcta tgccgacgtc
     6061 gtcgtgctcc ggcaccccca gcctggagca gtggagctgg ccgccaagca ctgccggagg
     6121 ccagtgatca atgctgggga tggggtcgga gagcacccca cccaggccct gctggacatc
     6181 ttcaccatcc gtgaggagct gggaactgtc aatggcatga cgatcacgat ggtgggtgac
     6241 ctgaagcacg gacgcacagt acattccctg gcctgcctgc tcacccagta tcgtgtcagc
     6301 ctgcgctacg tggcacctcc cagcctgcgc atgccaccca ctgtgcgggc cttcgtggcc
     6361 tcccgcggca ccaagcagga ggaattcgag agcattgagg aggcgctgcc tgacactgat
     6421 gtgctctaca tgactcgaat ccagaaggaa cgatttggct ctacccagga gtacgaagct
     6481 tgctttggtc agttcatcct cactccccac atcatgaccc gggccaagaa gaagatggtg
     6541 gtgatgcacc cgatgccccg tgtcaacgag ataagcgtgg aagtggactc ggatccccgc
     6601 gcagcctact tccgccaggc tgagaacggc atgtacatcc gcatggctct gttagccacc
     6661 gtgctgggcc gtttctag
//
