LOCUS       sequence-329-exp       10665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10665)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 10665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD187.24
FEATURES             Location/Qualifiers
     source          1..10665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7827..7845)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7866..7882)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7866..7882)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7879..7901)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7890..7906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7914..7920,7921..7938,7939..7944))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(8097..8114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8268..8856)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9027..9818,9819..9887))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9650..9669
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9888..9992)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10019..10474)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10156..10177)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10368..10387
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10601..10623
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10615..10632
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10616..10632
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10639..10658
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10639..10657
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctccacc gcggttttgg cgcggtggcg gccgcgcgga agatctggaa gtaaaatatt
       61 gtaatatttt ccctaaattt cacttaaaaa taaggatttt tgcaacgtaa aacctcaaaa
      121 attcgctgaa attgacgatt tttgttcaaa attttgaaaa acagggttaa ttttagctga
      181 aaatcttcaa ttttgccaga aattggcttt ttttccgtcg ccacacggag aacttgccaa
      241 aaatgaagac ttttagccaa tttagccctg aaaattcgag ttttgggcga tttttggtct
      301 gagaagccgt tttcgcagta tattatattt tctcgttaaa tttcatttta aaataaacat
      361 tttgaacgta taaccccaaa aattcgctgc gcaatggatg atttttgtcc aaagttttga
      421 taattttagc taaaaacctc gaattttggc aatttttcgt gccgcatcgg ccgaaagttg
      481 actttttttg gaattatcat aatttaatgc acattttggt agtttatctc acataatctg
      541 tctgcaggcg ggaaaaattt gagataaact actaaaagat gcaaaacaag gatgataatt
      601 ccagaaaatc aacttcggac cgtctcgaca cggaaaattg agcgaaattg gagttttaaa
      661 atgatttagc cctgaaaatt cgagaaattt aggcggtttt gggtctgaaa aggcgttttc
      721 gcagtatttt ctcgctaaat ttcatttatt atcacataat atcagtactt aggcgaatat
      781 cttgtagaaa tgttggtttt gttcgataat tctctacttt tagagtcgat tcacgagaaa
      841 atgttgcacc atcttacttt tttcccttgt aaaacataga ttcttctgta caatgaaaga
      901 gaacacaatt tcttcgtgaa gcaactcaaa aagtagagaa aattcgaaca aaacccaaca
      961 tttctacaag atattcgtct aaatagtgat ataatgtgtt aataagcata ttttaaatga
     1021 aatttagcga aaaaactgcg aaaatttgtc aaaaaagcga atttccatcc gatcagcaga
     1081 tctctaaaag ttacataaaa ttgaaagttt tgtggggaaa ttgttttaaa acacgcctat
     1141 ggtaatacga gaacataaaa ttctgagaat gcgtattaca caacatattc gacgcgcaaa
     1201 atataactat atctcgtagc gaaaactaca gtaatcctta aatgactact gtagcgattg
     1261 tgtcgattta cgggctcgat tctcggaaaa aaaaatcatt tttcaaattt tgacagcgat
     1321 attcaatttt tcttcgtttt ttcgtatttc tcagtcattt ttgtgcttat ttttaatatt
     1381 ttattcatta ataaattaat ttaattgaaa aacgtccaga cgtattttta catttcccgt
     1441 ctatttccgt aataggcggt taataacgta gaaatacgtc tgtatgagtt tactcatcaa
     1501 aatgacacga atattggacg tttttaaatc aaattaattt attaataggt aaaatattaa
     1561 aaaataaaca caaaaatgac aaaggaatag gaaaacacga agagaaattg agtatcgctg
     1621 tcaaaattcg aaaaacgtgg atttttccaa gaatcgagcc cgtaaatcga cacattcgct
     1681 acagtagtca tttaaggatt attgtagttt tcgctacgag atactttgcg cgccaaatat
     1741 gttgtgaaat acgcattctc agaattttat gttcccgtaa taccacaatg accgaatatc
     1801 ataataaaaa aattcaaaaa caatttctag attttatatg attttttgaa aattgaaaaa
     1861 atctcagttt tcacctaatt atatttgaat taccgccaat tgaactcgtt cgttggagcg
     1921 cgcttgcatt attttcatta attaatttta ttaattttca ttatttcact gattttcttc
     1981 atttttgagg ttttttttat cgggaaatga aagaaatata caagaaaaat gcaaaatgtt
     2041 tattaaaaac tgaaattagt atttctcgga gttttaggca ttttcagtta ctttttaata
     2101 aagattttgc atttttcttg tatatttctt tcattttccg attaaaaaac cccaaaaatg
     2161 aagaaaatca gtgaaataat gaaaattaat tcaaataact aatgaaaata atgcaagcgc
     2221 gctccaacca acgagttcaa ttggcggtaa ttcaaatata attaggtgaa aactgagatt
     2281 ttttcaattt tcaaaaaatc atataacatc tagacaattt ttttgaattt ttttatcatg
     2341 atattcggtc attgtgtccc cataggcatg ttttaaagca atttccacct ttaaataaaa
     2401 tcgagaaaaa atggcgtcaa aagacatatg taaaagctgt ttctctttgt atattcacac
     2461 agaatatagc ccgattttgg aggtgaaaaa ccggtttttt tgtattcttt acgccacata
     2521 aagtgatatg gaagagaaaa tcgacatatt tatgtacttg gagtacgagt acattggaga
     2581 aaaagggtga actactggaa aatctgggaa atttcagaaa aaaattccga aaaatctttt
     2641 ttgctgaaaa aaaaatttgt tcgaaatttg ggaaatactg gaaaattttc atcaaaatta
     2701 aagcgatttt taacgttttt gcttgggaga tactatttgt aattttaatg tttggtgagg
     2761 acaacagaaa tgaccttcag cgtacggaaa acgattaaaa gacacatttg aatcgaaaat
     2821 agcgtgaaaa acatgaaaat atcggaaaaa tagctttaaa attggatttg aaagcaaaaa
     2881 atgtatcaaa aatcgagaaa aatgcttgaa aaagttgaaa aactcaaaca aaaaatcaaa
     2941 aaaggttttt ttaaaaatct gtgagtatcg caacgaaagc acaacttgag gcatgcgcct
     3001 ttaaaccaac cgtaaccaaa ttcggaggca aaaaaagatt tctcgctgtt ttttcagttt
     3061 taaaatcact tttcacgttt cttttcgcag taattcgttt gcaaaacatc tgaaaaaatt
     3121 ttattttata agaaattata taaaaatcgc tataaattga gtttttgccc ccaaattcag
     3181 cacggagcac ttctcaatta aatataattt tattttttaa cattgaaagc tttcataaaa
     3241 ttgagctttg tttgtaaata atcagtgaaa aaacacagaa atcttcaaaa aataaagaaa
     3301 aagtttttaa aaatctgtga gtcccgcaac gaaaataaaa ctttaggcat gcgcctttaa
     3361 accaaccgta accaaattcg gcggcaaaaa tggatttctc gccgtttttt cagttttaca
     3421 atcacttttt aggtttcttt ttgcagtatt tcgtttccca aacaattaaa aatcaaattt
     3481 tcttttccag atagctcaaa caggatccta tcgattcgcg gccgctgtac acccggtaga
     3541 aaaaatgtca aaaggagagg aattgttcac cggcgtcgtt ccaatcctcg ttgaactcga
     3601 cggcgacgtc aacgggcaca agttttccgt ctctggagag ggagagggag acgcaaccta
     3661 cggcaaattg acccttaaat tcatttgcac cacaggtaag tttcgatttt tgctatcttt
     3721 ttcacgattt ttactctctt tttctactaa ctaacctcct ttttgaacat tttttcagga
     3781 aaattgccag ttccatggcc aacattggtc acaaccttct gttacggcgt ccaatgcttc
     3841 tcgcgttacc ccgatcacat gaaaaggcac gactttttta aatccgcaat gccagaagga
     3901 tacgtacaag aacgcacaat tttcttcaaa gatgacggaa actacaagac aaggtaagtt
     3961 tttcccaact ttttcggact ttttgaccgc tttttatact aactaacgga ctttttcgtc
     4021 ctttttcagg gccgaagtca agtttgaagg agacaccctc gtcaaccgca tcgaacttaa
     4081 aggaattgac ttcaaagaag acggaaacat tctcggacac aaactcgaat acaactacaa
     4141 ttctcacaac gtttacataa tggccgacaa acaaaagaac ggaatcaagg taagtttcgc
     4201 tttttgatga tttttggacc atttttagga atttttcgat actaactaac tatttttgcc
     4261 tgctttttca ggtcaacttc aagattcgtc acaacattga ggacggaagc gtccaacttg
     4321 ctgaccatta ccagcaaaac accccgattg gcgacggacc cgtcctcctt ccggacaacc
     4381 actacctctc aacacagagc gccctctcga aagacccgaa cgagaagagg taagtttatc
     4441 gtttttcgac gatttttacc catttttacg ccatttttga tactaactaa cgccttttta
     4501 ccgatttttc agggaccaca tggtcctttt ggaatttgtt acggccgcag gcatcaccca
     4561 cggcatggac gaactctaca aatagcattc gtagaattcg ctagccggcc atacaagtaa
     4621 tccggataat tttgccgtat tttccatatt ttgttttgta tatttatcca ctcaccccct
     4681 ctctttgtcc tgtgaatgaa cttgtgccaa aaaagccaaa aataatttac tttttttaaa
     4741 gacatattaa aacttgaaaa cttggaacaa tacggtgcga aagcgaaaca gagacagaaa
     4801 aatgaacaaa gagatccgtg aaaagtgaac gatgatttag gcttcttgtt gggcggcgtg
     4861 gatgagagtg atgttgtctc ctttgagaag aattctgccg atcttgttgc gtcccttcgt
     4921 cttcatgttc acctcctcgg cttcgtcgaa cacgacattc atgaactcgt cgaatccgat
     4981 gatgtagccc tcgagacggt gggtgacatc ctcgtacaac cagatttgga cccgagtgcg
     5041 gttttggaga tatctgaaat ttgggaaatt gttttctact gattagtgaa aaaatattac
     5101 ctgaagatga ggttcacagg ctgaaccatc actttgttga gctttctcgt cgacattttt
     5161 gcgggatgat ctgtaaaaat caatatattt tttgaaagac acaaattttt aagataaata
     5221 tcaagaaaac tcgattttag cagctattta agctcaaaaa ctacaattta tggctttaaa
     5281 acataatttt tcacatttta cataaatttc aacgaaaaaa aatgccaaaa aacttcaaat
     5341 ttgagcagaa atcggctatt ttctcgacaa aaataatttc gaaaattcgt tcaaaaaaga
     5401 tgagtacgca agcaccgtga cgcacagttg cggactttgt aaaccgtacc cctgcataaa
     5461 aaggcggaat tttgaattta ataaaatgca gtttagaaca aagaaaagca cggagagaat
     5521 tgtgataact aattatttgt atttaatttt aaatactaca taaaactgaa gattggaacg
     5581 agttaaagct ggtatttgcg gtttatagcc ccaaaagtta aggcttgtta ggcaattatc
     5641 gataaaaaca acgagaaatt cgtgaaatcg gcaaaaattt atagaaaacg tttgaattaa
     5701 attaaattta cgcttttgcc gccaacacct atcgagaccc tagttatagg ggcggagcgt
     5761 catgggagcg cgcttgcaac tcgatttcaa aaagtttcgc tgtttgtaca acattctcag
     5821 ttgttcttaa tcagttaaat aagtttcaaa attttcaaaa tcataaaaca cagttaatgc
     5881 taaattaaag tctaaaaaat caggtgataa ggggtttgga cgaccaaaaa gcgaccttcg
     5941 ctcgaaaatt gataaactaa ctacactttt tccggaaaaa tgatggaaaa atcctgattt
     6001 tcttcgttga atcaattttt gtttcatttt taacactttc acatgaaata tttcagttaa
     6061 aatgctcaag ctaattgggt ttcttataat tttgggtctc gcaacggccg acaagtgctc
     6121 agattgtttg gctcagggga aacagtgagt tggaagctta caattctgag aattcgtatt
     6181 gcgcagctta tttgacgcgc aaaatatgaa atatcacgta gcgaaaacta cagtaattct
     6241 ttaaataaga ctactgtagt gcctatgtcg atttactgcc tcaactttct taatcaagct
     6301 cgtaaatcga caccagctct acagtagtca tagttttcgc tacgagatat tttgcgcgtc
     6361 aaatatggtg tgcaatacgc attttcagaa ttttatgttc tcgtaatata ccttttttca
     6421 gctggtgtgt tactgtaaat gcatgtggat ttacgccatg tgtcactaca attacagtga
     6481 gtttttttga agaaaaaaaa attacccatt tgatcgccaa ttccattttt caaaaatcaa
     6541 taaaaaaaaa aaatttcggc gatcagaaac gttacagaaa gccctaaact gcccggaact
     6601 accagataaa gcccacgctt acgatgattc atttatgagg aataaggtat tgatcacaca
     6661 ttctgctgcc tacaatatgg acccacaaaa gtgttttacg taggttcacc ttctagataa
     6721 accttaaaaa tcgatacttt cagtagtaaa atgccttcga tgaaagtttc caaaacagtt
     6781 aatgtcaatt gtgatagata tgggccaagt gagttgtttt attagcccac ggatttctgg
     6841 cttccctcat aaattgaaaa ggaagagatt gccgaactag accattttgg ctcggcgcgt
     6901 tgcgtgtcac gtcgcggctc gattgtaaaa ctaaatgtat tggtccgtgt ggagtacacg
     6961 actttccctt tccggcaggc gattgtcaat ggagcgcgaa aaattcaatg aggaaggcca
     7021 gaaccccgtg tctgtagttt tctcaaaaag tggcaaaaac tcaataattg ccaatttttg
     7081 actgccaata aaaatttcac taaatttatt aaatttattt taagttttat tgaaattttt
     7141 ttaaaatttc cccactggcg ctaaaaaatc caattttcag aagcaatgtg cttcagcttc
     7201 acggccatag atactgtcca aaaagtgatt gcaatgacgt ttagagggac agaaggagca
     7261 gctcaattgt ctgaagaaat tttggatttc ttccagtaag ttcgggaaat ggcaatttaa
     7321 agtttaattg gatttttgta ttttttcttc tcacgactac agttttgcac atttttatgt
     7381 tataaaaaac caaaaaaaaa tttttttgcc ttttttctca gcttaccaca aaacaatttc
     7441 gaccaaaaat aaattgaaaa aattaaaaaa ttttttgcac ggttttcgga ctcaaaaaaa
     7501 gctgaatgtt cctggaacta tttgtaaaat tcaaaattca aaattttcac gaattctcta
     7561 aaaggttcca gcaaattcca caactttctt gaatttttca aaaaagttct tgagcattca
     7621 acaattattt ttggaatttt ccaaaggtta caggacgttc caatttttca aaaaaatttc
     7681 tcgcaaaaaa tgttttctca gaaaatttga atttccctcc acataaaaga ttgttctcaa
     7741 aaaatttgca aacacacatt ttcagcggaa aaaaggcgtt cttcgactct ggaagtgtat
     7801 tcgaatatgg tacccagctt ttgttccctt tagtgagggt taattccgag cttggcgtaa
     7861 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7921 ggagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta actcacatta
     7981 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8041 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8101 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8161 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8221 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8281 ggcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8341 ggactataaa gataccaggc gttcccccct ggaagctccc tcgtgcgctc tcctgttccg
     8401 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8461 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8521 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8581 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8641 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8701 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8761 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8821 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8881 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8941 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9001 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9061 gcgatctgtc tatttcgttc atccatagtt gcctgactgc ccgtcgtgta gataactacg
     9121 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9181 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9241 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9301 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9361 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9421 tgatccccca tgttgtgaaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9481 agtaagttgg ccgcagtgtt atcactcatg cttatggcag cactgcataa ttctcttact
     9541 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9601 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9661 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9721 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9781 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9841 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9901 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9961 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgaa
    10021 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
    10081 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
    10141 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
    10201 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
    10261 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
    10321 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
    10381 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
    10441 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctacgcaac
    10501 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaagggggga
    10561 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    10621 acgacggcca gtgaattgta atacgactca ctatagggcg aattg
//
