LOCUS       sequence-32900-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Lowary PT, Widom J
  TITLE     New DNA sequence rules for high affinity binding to histone octamer 
            and sequence-directed nucleosome positioning.
  JOURNAL   J Mol Biol. 1998 Feb 13. 276(1):19-42.
  PUBMED    9514715
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            1998 Feb 13. 276(1):19-42."
COMMENT     Sequence Label: pGEM-3z/612
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(357..375)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(358..375)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(393..409)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(393..409)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(406..428)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    417..433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(441..447,448..465,466..471))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    486..507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(624..641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(875..894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcctatg ggcgattcga gctcggtacc cggggatcct ctagagtccg ggatcctaat
       61 gaccaaggga tctatagagc tctagaatat ttatggagct gaaaactgct cccccacacc
      121 ctatactgcc cgcgtaagaa ggtgccgact atgtccgcgg aaattaagag gtattaaacg
      181 tgcagctgga atgctctaga cccctttagg agccgctaga ggccaaggct agcatattag
      241 ctcggtagtt tggcctcaat caattgggtt cacgccgaac tggacatacc gatttggaat
      301 ggtgccagtc ggatagtgtt ccgagctccc acctgcaggc atgcaagctt gagtattcta
      361 tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      421 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      481 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      541 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      601 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      661 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      721 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      781 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      841 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      901 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgcctt
//
