LOCUS       sequence-32901-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  TITLE     Gerardo Morfini plasmids
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FCbAIGW
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(1..9,10..12,13..587)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(174..193)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     403..420
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     misc_feature    589..641
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 ggttgtggcc atattatcat cgtgtttttc aaaggaaaac cacgtccccg tggttcgggg
       61 ggcctagacg tttttttaac ctcgactaaa cacatgtaaa gcatgtgtac cgaggcccca
      121 gatcagatcc catacaatgg ggtaccttct gggcatcctt cagccccttg ttgaatacgc
      181 ttgaggagag ccatttgact ctttccacaa ctatccaact cacaacgtgg cactggggtt
      241 gtgccgcctt tgcaggtgta tcttatacac gtggcttttg gccgcagagg cacctgtcgc
      301 caggtggggg gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt cttcaagaag
      361 cttccagagg aactgcttcc ttcacgacat tcaacagacc ttgcattcct ttggcgagag
      421 gggaaagacc cctaggaatg ctcgtcaaga agacagggcc aggtttccgg gccctcacat
      481 tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac cggccttatt
      541 ccaagcggct tcggccagta acgttagggg ggggggaggg agaggggcgg atcccgggcc
      601 cgcggtaccg tcgactgcag aattcgaagc ttgagctcga ga
//
