LOCUS       sequence-329019-        4782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri DDX1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-DDX1
FEATURES             Location/Qualifiers
     source          1..4782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2907..3006)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3023..3042)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3024..3042)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3047..3063)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3047..3063)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3165..3184)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3176..3985
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3243..3262)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4132..4720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4621..4640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatggc agccttttct gaaatgggag ttatgcctga
      721 aatagcgcag gctgtggaag agatggactg gctactaccc acggacatcc aggcagagtc
      781 tattcccctg attctcggtg gaggagatgt actcatggct gcagaaaccg gaagtggcaa
      841 aacaggagcg ttcagtatcc cagtgattca gattgtgtat gagaccataa aggatcagca
      901 ggagggcaag aagggcagag ctccagtcaa aactggagga actattttca acacctggca
      961 gatgaaccca tatgatcgta gcacacagtt tgctatcgga cctgatggcc tgtgctgtca
     1021 gagcagggag tttaaagagt ggcacggctg tcggtccact aaaggcgtca caaaaggaaa
     1081 gtactactat gaagtgagct gtcaggacca gggtttgtgc cgtatcggat ggtccaccag
     1141 tcaagcagca ctggatctgg gaactgataa gtatggcttt ggctttggcg gcactggaaa
     1201 gaagtccaac aacaagcagt ttgacagcta tggagaggag ttcaccatgc atgacacgat
     1261 cgggtgctac ctggatctag acaagaatca gatttcattc tctaaaaatg gaaacgacct
     1321 gggtttggca tttgaaatcc ctcaaaacct gaggaatcag gctttcttcg cctcctgcgt
     1381 tctcaagaat gctgagctga agtttaactt tggaggagaa cattttaaat tcccacctaa
     1441 ggaagggttt gtggctctgg atcaggcatc ggagggccac acagtcaaat cttcacagac
     1501 aggcagtgcc aaagtgagtc aagtgaaaac gtcatcaaat gctcccaaag ccctgatcat
     1561 tgagccgtct aaagagctgg cagagcaaac cttcaacaac gtcaagcagt ttaaaaaata
     1621 tgtggataat cctaaattaa gggagcttct ggtgatcggt ggtgtggcag ccaaagaaca
     1681 gctggctgtg ctggaacagg gggtggacat agtggtggga acgcccggca ggctggatga
     1741 cctcatctcc accgggaagc tgagtctggc ccaagtccgt tttctggtcc tggatgagtg
     1801 tgatggcctc ctgattgcag gctacactga ttttatcaac aggatccata aacaaatccc
     1861 tcaggtgaca tccgatggca aaaggctgca ggtcatcgtg tgttcggcca cacttcactc
     1921 ctttgatgtg aagaagctgt ctgaacgcat catgcacttt cccacctggg tggacctgaa
     1981 gggggaggac tcggttccag agaccgtcca tcatgtggtg gtccccgtca acccaaagac
     2041 cgaccgtctg tgggaacgcc tcgggaagaa ccacatccag acggatgaag tccatgccaa
     2101 agacaacacc cgaccaggag tcggttctcc agaaatgtgg tcagaagcta taaaggtgct
     2161 gaagggggag tacacgatcc gagcaattaa agaacacaaa atggaccaag ccataatctt
     2221 ctgccgcacc aagatcgact gtgacaacat ggagcagtac ttcatccagc atggaggagg
     2281 ccccgacagc aaaggtcacc agttatcctg cgtttgtctt cacggtgatc ggaagcccaa
     2341 tgagaggaaa accaacttgg agcgattcaa gagaaaagaa gtgaggctgc tgatctgcac
     2401 agacgtagcc gccagaggca tcgacatcca cggcgttccc tatgtgataa acgtcaccct
     2461 gccagacgag aagcagaact acgtccaccg catcggcaga gtcggacgag ctgagagaat
     2521 gggacttgct atctctctgg tggccatgga gaaagaaaag gtgtggtacc acgtgtgccc
     2581 gaacagaggc agaggctgct acaacacccg gctgaaggaa gacggaggct gcaccatttg
     2641 gtacaacgag aaggagctct tgtcagacat cgaggagcac ctgaagtgca ccatcactca
     2701 gtgtgagcca gatatcaaag taccagtgga tgagtttgat gggaaggtga cctacggaca
     2761 gcgcagggct ttaggaggtg gaaactacaa gggccatgtg gatgtcctgg ccccaacagt
     2821 ccaggagctg gcccttctag agagggaagc tcagacgtcc ttcctccacc tgggatacct
     2881 gccaaaccag ctgttcagag ccttcgaccc agctttcttg tacaaagttg gcattataag
     2941 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     3001 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     3061 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     3121 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     3181 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     3241 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     3301 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     3361 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     3421 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     3481 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     3541 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     3601 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     3661 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     3721 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     3781 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     3841 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     3901 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     3961 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     4021 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     4081 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4141 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4201 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4261 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4321 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4381 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4441 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4501 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4561 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4621 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4681 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4741 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
