LOCUS       sequence-329035-        3671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human MLST8 [NM_022372.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3671)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E134 Hs.MLST8-nostop
FEATURES             Location/Qualifiers
     source          1..3671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    46..145
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1124..1223)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1600..2391
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2484..3072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2973..2992
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3226..3243
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3402..3429)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3521..3607)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(3656..3671,1..7)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3670..3671,1..16)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(3671,1..16)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taaaacgacg gccagtctta aaattcgagc tcggtccgag gatccgaaat aatgatttta
       61 ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt ttttataatg
      121 ccaactttgt acaaaaaagt tggcaccatg aacacctccc caggcacggt gggcagtgac
      181 ccggtcatcc tggccactgc aggctacgac cacaccgtgc gcttctggca ggcccacagc
      241 ggcatctgca cccggacggt gcagcaccag gactcccagg tgaatgcctt ggaggtcaca
      301 ccggaccgca gcatgattgc tgctgcaggt taccagcaca tccgcatgta tgatctcaac
      361 tccaataacc ctaaccccat catcagctac gacggcgtca acaagaacat cgcgtctgtg
      421 ggcttccacg aagacggccg ctggatgtac acgggcggcg aggactgcac agccaggatc
      481 tgggacctca ggtcccggaa cctgcagtgc cagcggatct tccaggtgaa cgcacccatt
      541 aactgcgtgt gcctgcaccc caaccaggca gagctcatcg tgggtgacca gagcggggct
      601 atccacatct gggacttgaa aacagaccac aacgagcagc tgatccctga gcccgaggtc
      661 tccatcacgt ccgcccacat cgatcccgac gccagctaca tggcagctgt caatagcacc
      721 ggaaactgct atgtctggaa tctgacgggg ggcattggtg acgaggtgac ccagctcatc
      781 cccaagacta agatccctgc ccacacgcgc tacgccctgc agtgtcgctt cagccccgac
      841 tccacgctcc tcgccacctg ctcggctgat cagacgtgca agatctggag gacgtccaac
      901 ttctccctga tgacggagct gagcatcaag agcggcaacc ccggggagtc ctcccgcggc
      961 tggatgtggg gctgcgcctt ctcgggggac tcccagtaca tcgtcactgc ttcctcggac
     1021 aacctggccc ggctctggtg tgtggagact ggagagatca agagagagta tggcggccac
     1081 cagaaggctg ttgtctgcct ggccttcaat gacagtgtgc tgggctcaac tttcttgtac
     1141 aaagttggca ttataggaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     1201 agtcaaaata aaatcattat ttgcatgcaa gcttagatcc tctagaccag ccaggacaga
     1261 aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg
     1321 aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     1381 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     1441 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     1501 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     1561 acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc
     1621 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     1681 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     1741 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     1801 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     1861 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     1921 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     1981 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     2041 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     2101 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     2161 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     2221 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     2281 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     2341 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc
     2401 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2461 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2521 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2581 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2641 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2701 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2761 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2821 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2881 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2941 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3001 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3061 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3121 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3181 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3241 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3301 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3361 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     3421 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     3481 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     3541 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     3601 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     3661 tcacgacgtt g
//
