LOCUS       sequence-329044-        4788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses prematurely truncated woolly monkey retroCHMP3 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Rheinemann L, Downhour DM, Davenport KA, McKeown AN, Sundquist WI, 
            Elde NC
  TITLE     Recurrent evolution of an inhibitor of ESCRT-dependent virus budding
            and LINE-1 retrotransposition in primates.
  JOURNAL   Curr Biol. 2022 Feb 25. pii: S0960-9822(22)00240-8. doi: 
            10.1016/j.cub.2022.02.018.
  PUBMED    35245459
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2022 Feb 25. pii: S0960-9822(22)00240-8. doi: 
            10.1016/j.cub.2022.02.018."
COMMENT     Sequence Label: pEF1-alpha-woolly-monkey-retroCHMP3(148)-HA
FEATURES             Location/Qualifiers
     source          1..4788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(547..1338,1339..1407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1170..1189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1408..1512)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1574..2756
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1805..2747
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2704..2724
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3233..3259
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3348..3367)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3413..3468
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3467..3486)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3829..3845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3829..3845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3842..3864)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3853..3869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3877..3883,3884..3901,3902..3907))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3922..3943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4002..4197
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4048..4183
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4203..4337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4240..4259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4294..4313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4405..4422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(4576..4788,1..376))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4656..4675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
       61 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      121 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      181 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      241 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      301 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      361 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      421 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      481 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
      541 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
      601 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
      661 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
      721 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
      781 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
      841 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
      901 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
      961 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1021 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1081 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1141 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1201 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1261 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1321 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1381 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1441 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     1501 aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga ttattgacta
     1561 gatcatcgcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     1621 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     1681 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     1741 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     1801 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     1861 cgtgccttga attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc
     1921 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     1981 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     2041 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     2101 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     2161 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     2221 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     2281 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     2341 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     2401 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     2461 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     2521 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     2581 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     2641 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     2701 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     2761 ttctgcagtc gacggtaccc aatttgccat ggggctgttt ggaaaaaccc aggagaagcc
     2821 accaaaagaa ctgctcaatg aatggtcatt gaagataaga aaggaaatga gagttgttga
     2881 caggcaaata agggatatcc aaagagaaga agaaaaagtg aaacgatctg tgagagatgc
     2941 agccaggaag agccagaagg atgtctgcat agttctggcc aaagagatga tcaggtcaag
     3001 gaaggccgtg agcaagccat atgcatccaa agcgcccatg aacttggtgc tcatggggat
     3061 gaggagccag ctcgcggtct tgcgagtggc tggttccctg cagaagagca cagaagtgat
     3121 gagggccgtg caaagtcttc tgaagattcc agaaattcag gccaccagga gggagctgtc
     3181 caaagaaatg atgaaggctg ggctcataga ggagatgtta gaggacactt tctacccata
     3241 cgatgttcca gattacgctt gagcggccgc actcctcagg tgcaggctgc ctatcagaag
     3301 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     3361 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     3421 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     3481 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     3541 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     3601 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     3661 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     3721 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     3781 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     3841 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3901 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3961 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     4021 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4081 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4141 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4201 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4261 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4321 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4381 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4441 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4501 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4561 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4621 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4681 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4741 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggta
//
