LOCUS       sequence-329045-        4788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses prematurely truncated pygmy marmoset retroCHMP3 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Rheinemann L, Downhour DM, Davenport KA, McKeown AN, Sundquist WI, 
            Elde NC
  TITLE     Recurrent evolution of an inhibitor of ESCRT-dependent virus budding
            and LINE-1 retrotransposition in primates.
  JOURNAL   Curr Biol. 2022 Feb 25. pii: S0960-9822(22)00240-8. doi: 
            10.1016/j.cub.2022.02.018.
  PUBMED    35245459
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2022 Feb 25. pii: S0960-9822(22)00240-8. doi: 
            10.1016/j.cub.2022.02.018."
COMMENT     Sequence Label: pEF1-alpha-pygmy-marmoset-retroCHMP3(148)-HA
FEATURES             Location/Qualifiers
     source          1..4788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(200..216)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(200..216)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(213..235)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    224..240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(248..254,255..272,273..278))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    293..314
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        373..568
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      419..554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    574..708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(611..630)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     665..684
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(776..793)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(947..1535)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1027..1046)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1706..2497,2498..2566))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2329..2348
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2567..2671)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2733..3915
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2964..3906
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3863..3883
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4392..4418
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4507..4526)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4572..4627
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4626..4645)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
       61 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
      121 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
      181 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      241 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      301 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      361 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
      421 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
      481 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
      541 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt
      601 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      661 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat
      721 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      781 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      841 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      901 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      961 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1021 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1081 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1141 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1201 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1261 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1321 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1381 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1441 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1501 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1561 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1621 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1681 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1741 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1801 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1861 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1921 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1981 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2041 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2101 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2161 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2221 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2281 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2341 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     2401 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     2461 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2521 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2581 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2641 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt
     2701 cgacattgat tattgactag atcatcgcgt gaggctccgg tgcccgtcag tgggcagagc
     2761 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     2821 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     2881 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     2941 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     3001 ttacgggtta tggcccttgc gtgccttgaa ttacttccac gcccctggct gcagtacgtg
     3061 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     3121 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     3181 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     3241 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     3301 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     3361 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     3421 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     3481 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     3541 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     3601 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     3661 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     3721 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     3781 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     3841 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     3901 tttcaggtgt cgtgaggaat tctgcagtcg acggtaccca atttgccatg gggctgttgg
     3961 ttaaaaccca ggagaagcca ccaaaagaac tggtcaatga atggtcattg aagataagaa
     4021 aggaaatgag agttgttgac aggcaaataa gggatatcca aagagaagaa gaaaaagtga
     4081 aacgatctgt gaaagatgca gtcaagaagg gccagaagga aatgtgcacg gttctggcca
     4141 gggagatgat caggtcaggg aaggctgcga gcaagctgta tgcatccaaa gcgcccatga
     4201 actcagtgct catggggatg aggaaccagc tcgaggtctt gcgaatggct ggttccctgc
     4261 agaagagcac agaagtgatg agggccatgc agagtcttgt gaagattcca gaaattcagg
     4321 ccaccatgag ggagctgtcc aaagaaatgg tgaaggctgg gatcatagag gagatgttag
     4381 aggacacttt ctacccatac gatgttccag attacgcttg agcggccgca ctcctcaggt
     4441 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     4501 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     4561 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     4621 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     4681 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     4741 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttatt
//
