LOCUS       sequence-329048-       10894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express C-terminal YN fused flock house virus (FHV) B2 protein 
            (2741-2956 nt) for dsRNA labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Cheng X, Deng P, Cui H, Wang A
  TITLE     Visualizing double-stranded RNA distribution and dynamics in living 
            cells by dsRNA binding-dependent fluorescence complementation.
  JOURNAL   Virology. 2015 Nov;485:439-51. doi: 10.1016/j.virol.2015.08.023. 
            Epub 2015 Sep 7.
  PUBMED    26351203
REFERENCE   2  (bases 1 to 10894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2015-11"; volume: "485:439-51. doi"; pages: " 
            10.1016/j.virol.2015.08.023. Epub 2015 Sep 7"
COMMENT     Sequence Label: pdRBFC-B2-YN
FEATURES             Location/Qualifiers
     source          1..10894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2594..2614
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    2681..2735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2740..2766
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2770..2794
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3020..3044)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3064..3066,3067..3069,3070..3582)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(3109..3130)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3370..3389)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     terminator      3664..4371
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(4396..4413)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4396..4412)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4405..4427)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4615..4639
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5940..6569
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6998..8071
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8137..8331
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8567..8589)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8675..8815
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8730..8749
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8830..8847)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9001..9589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9081..9100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9676..10470)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa
     2701 acaacaaaca acattacaat tacatttaca attaccatgt acccatacga tgttccagat
     2761 tacgctatca caagtttgta caaaaaagca ggctgcatga tgccaagcaa actcgcgcta
     2821 atccaggaac ttcccgaccg cattcaaacg gcggtggaag cagccatggg aatgagctac
     2881 caagacgcac cgaacaacgt gcgcagggac ctcgacaacc tgcacgcttg cctaaacaag
     2941 gcaaaactaa cggtaagtcg gatggtaaca tcactgctgg agaaacccag cgtggtggca
     3001 tacctagagg gaaaggccga cccagctttc ttgtacaaag tggtgcctag gagtatcgct
     3061 acaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3121 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3181 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3241 accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
     3301 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3361 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3421 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3481 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     3541 aacggcatca aggtgaactt caagatccgc cacaacatcg aggactgacc taggtgagtc
     3601 tagagagtta attctaggtg agtctagaga gttaattaag acccgggact agtccctaga
     3661 gtcctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct
     3721 gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg
     3781 ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt
     3841 caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt
     3901 gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt
     3961 tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg
     4021 attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc
     4081 gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg
     4141 tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc
     4201 aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt
     4261 tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat
     4321 cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca ggacctgcag
     4381 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4441 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4501 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
     4561 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
     4621 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     4681 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     4741 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     4801 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     4861 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     4921 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     4981 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     5041 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     5101 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     5161 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     5221 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     5281 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     5341 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     5401 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     5461 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     5521 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     5581 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     5641 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     5701 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
     5761 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     5821 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     5881 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     5941 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     6001 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     6061 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     6121 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     6181 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     6241 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     6301 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     6361 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     6421 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     6481 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     6541 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     6601 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     6661 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     6721 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     6781 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     6841 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     6901 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct
     6961 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     7021 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     7081 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     7141 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     7201 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     7261 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     7321 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     7381 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     7441 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     7501 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     7561 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     7621 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     7681 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     7741 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     7801 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     7861 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     7921 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     7981 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     8041 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     8101 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
     8161 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
     8221 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
     8281 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
     8341 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
     8401 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
     8461 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
     8521 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     8581 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     8641 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     8701 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     8761 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     8821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     9481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9661 catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca
     9721 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
     9781 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
     9841 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
     9901 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
     9961 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
    10021 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
    10081 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
    10141 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
    10201 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
    10261 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
    10321 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
    10381 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
    10441 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
    10501 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
    10561 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
    10621 aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt
    10681 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
    10741 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
    10801 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
    10861 cgagctgccg gtcggggagc tgttggctgg ctgg
//
