LOCUS       sequence-329055-        7294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid pJo55, which contains positive control (C. annuum eIF4E-1) 
            for complementation test in Jo55 yeasts..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Lebedeva MV, Nikonova EY, Terentiev AA, Taranov VV, Babakov AV, 
            Nikonov OS
  TITLE     VPg of Potato Virus Y and Potato Cap-Binding eIF4E Factors: 
            Selective Interaction and Its Supposed Mechanism.
  JOURNAL   Biochemistry (Mosc). 2021 Sep;86(9):1128-1138. doi: 
            10.1134/S000629792109008X.
  PUBMED    34565316
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochemistry (Mosc)"; date: "2021-09"; volume: "86"; issue: "9"; 
            pages: "1128-1138"
COMMENT     Sequence Label: pJo55-CA1
FEATURES             Location/Qualifiers
     source          1..7294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..644
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     538..561
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     650..666
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     650..666
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      1380..1627
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1396..1414)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(1662..1681)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1663..1681)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1691..1708)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1691..1707)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1700..1722)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1846..2301
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1933..1952)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2143..2164
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2561..3223)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(3224..3411)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(3558..3577)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3677..3695)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      3736..5078
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    4068..4115
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        5105..5209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5210..5278,5279..6070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5428..5447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6241..6829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6730..6749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6983..7000
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7117..7138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7153..7158,7159..7176,7177..7183)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7191..7207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7196..7218
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7215..7231
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7215..7231
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7250..7270
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7252..7270
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcattatcaa tactcgccat ttcaaagaat acgtaaataa ttaatagtag tgattttcct
       61 aactttattt agtcaaaaaa ttagcctttt aattctgctg taacccgtac atgcccaaaa
      121 tagggggcgg gttacacaga atatataaca tcgtaggtgt ctgggtgaac agtttattcc
      181 tggcatccac taaatataat ggagcccgct ttttaagctg gcatccagaa aaaaaaagaa
      241 tcccagcacc aaaatattgt tttcttcacc aaccatcagt tcataggtcc attctcttag
      301 cgcaactaca gagaacaggg gcacaaacag gcaaaaaacg ggcacaacct caatggagtg
      361 atgcaacctg cctggagtaa atgatgacac aaggcaattg acccacgcat gtatctatct
      421 cattttctta caccttctat taccttctgc tctctctgat ttggaaaaag ctgaaaaaaa
      481 aggttgaaac cagttccctg aaattattcc cctacttgac taataagtat ataaagacgg
      541 taggtattga ttgtaattct gtaaatctat ttcttaaact tcttaaattc tacttttata
      601 gttagtcttt tttttagttt taaaacacca gaacttagtt tcgacggatt ctagaactag
      661 tggatcccca aaatggcaac agctgaaatg gagaaaacga cgacgtttga tgaagctgag
      721 aaggtgaaat tgaatgctaa tgaggcagat gatgaagttg aagaaggtga aattgttgaa
      781 gaaactgatg atacgacgtc gtatttgagc aaagaaatag caacaaagca tccattagag
      841 cattcatgga ctttctggtt tgataatcca gtggcgaaat cgaaacaagc tgcttggggt
      901 agctcgcttc gcaacgtcta cactttctcc actgttgaag atttttgggg tgcttacaat
      961 aatatccacc acccaagcaa gttagttgtg ggagcagact tacattgttt caagcataaa
     1021 attgagccaa agtgggaaga tcctgtatgt gccaatggag ggacatggaa aatgagtttt
     1081 tcaaagggta aatctgatac cagctggcta tatacgctgc ttgcaatgat tggacatcaa
     1141 ttcgatcatg aagatgaaat ttgtggagca gtagttagtg tcagaggtaa gggagaaaaa
     1201 atatctttgt ggaccaagaa tgctgcaaat gaaacggctc aggttagcat tggtaagcaa
     1261 tggaagcagt ttctggatta cagcgacagt gttggcttca tatttcacga cgatgcaaag
     1321 aggctcgaca gaaatgcaaa gaatcgttac acagtataaa ctgggggctg caggaattct
     1381 catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga
     1441 aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt
     1501 agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta
     1561 cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt
     1621 aatttgcgtc gacctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt
     1681 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1741 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1801 gcaccgcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg
     1861 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     1921 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     1981 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2041 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     2101 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2161 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     2221 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2281 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2341 ttcacaccgc atagatccgt cgagttcaag agaaaaaaaa agaaaaagca aaaagaaaaa
     2401 aggaaagcgc gcctcgttca gaatgacacg tatagaatga tgcattacct tgtcatcttc
     2461 agtatcatac tgttcgtata catacttact gacattcata ggtatacata tatacacatg
     2521 tatatatatc gtatgctgca gctttaaata atcggtgtca ctacataaga acacctttgg
     2581 tggagggaac atcgttggta ccattgggcg aggtggcttc tcttatggca accgcaagag
     2641 ccttgaacgc actctcacta cggtgatgat cattcttgcc tcgcagacaa tcaacgtgga
     2701 gggtaattct gctagcctct gcaaagcttt caagaaaatg cgggatcatc tcgcaagaga
     2761 gatctcctac tttctccctt tgcaaaccaa gttcgacaac tgcgtacggc ctgttcgaaa
     2821 gatctaccac cgctctggaa agtgcctcat ccaaaggcgc aaatcctgat ccaaaccttt
     2881 ttactccacg cacggcccct agggcctctt taaaagcttg accgagagca atcccgcagt
     2941 cttcagtggt gtgatggtcg tctatgtgta agtcaccaat gcactcaacg attagcgacc
     3001 agccggaatg cttggccaga gcatgtatca tatggtccag aaaccctata cctgtgtgga
     3061 cgttaatcac ttgcgattgt gtggcctgtt ctgctactgc ttctgcctct ttttctggga
     3121 agatcgagtg ctctatcgct aggggaccac cctttaaaga gatcgcaatc tgaatcttgg
     3181 tttcatttgt aatacgcttt actagggctt tctgctctgt catctttgcc ttcgtttatc
     3241 ttgcctgctc attttttagt atattcttcg aagaaatcac attactttat ataatgtata
     3301 attcattatg tgataatgcc aatcgctaag aaaaaaaaag agtcatccgc taggtggaaa
     3361 aaaaaaaatg aaaatcatta ccgaggcata aaaaaatata gagtgtacta gaggaggcca
     3421 agagtaatag aaaaagaaaa ttgcgggaaa ggactgtgtt atgacttccc tgactaatgc
     3481 cgtgttcaaa cgatacctgg cagtgactcc tagcgctcac caagctctta aaacgggaat
     3541 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     3601 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     3661 caagctgtga caaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3721 atggtttctt agtatgatcc aatatcaaag gaaatgatag cattgaagga tgagactaat
     3781 ccaattgagg agtggcagca tatagaacag ctaaagggta gtgctgaagg aagcatacga
     3841 taccccgcat ggaatgggat aatatcacag gaggtactag actacctttc atcctacata
     3901 aatagacgca tataagtacg catttaagca taaacacgca ctatgccgtt cttctcatgt
     3961 atatatatat acaggcaaca cgcagatata ggtgcgacgt gaacagtgag ctgtatgtgc
     4021 gcagctcgcg ttgcattttc ggaagcgctc gttttcggaa acgctttgaa gttcctattc
     4081 cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaaa accaaaagcg
     4141 ctctgaagac gcactttcaa aaaaccaaaa acgcaccgga ctgtaacgag ctactaaaat
     4201 attgcgaata ccgcttccac aaacattgct caaaagtatc tctttgctat atatctctgt
     4261 gctatatccc tatataacct acccatccac ctttcgctcc ttgaacttgc atctaaactc
     4321 gacctctaca ttttttatgt ttatctctag tattactctt tagacaaaaa aattgtagta
     4381 agaactattc atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc ctatacgtag
     4441 tatatagaga caaaatagaa gaaaccgttc ataattttct gaccaatgaa gaatcatcaa
     4501 cgctatcact ttctgttcac aaagtatgcg caatccacat cggtatagaa tataatcggg
     4561 gatgccttta tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa
     4621 gtggagtcag gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc
     4681 ttaacggacc tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag
     4741 aaaaaaagta atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta
     4801 gaacaaaaaa gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg
     4861 ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt
     4921 ttgttttaca aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat
     4981 ttctgttctg taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg
     5041 catttttgtt ctacaaaatg aagcacagat gcttcgttca ggtggcactt ttcggggaaa
     5101 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5161 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5221 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5281 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5341 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5401 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5461 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5521 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5581 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5641 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5701 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5761 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5821 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5881 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggatctc gcggtatcat
     5941 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6001 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6061 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6121 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6181 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6241 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6301 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6361 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6421 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6481 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6541 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6601 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6661 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6721 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6781 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6841 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6901 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6961 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7021 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7081 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7141 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7201 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     7261 tcactaaagg gaacaaaagc tggagctcag ttta
//
