LOCUS       sequence-32906-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  TITLE     Gerardo Morfini plasmids
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FSynIGW2
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(17..25,26..28,29..602)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(190..209)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     419..436
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 cgcccttgct caccatggtt gtggccatat tatcatcgtg tttttcaaag gaaaaccacg
       61 tccccgtggt tcggggggcc tagacgtttt tttaacctcg actaaacaca tgtaaagcat
      121 gtgtaccgag gccccagatc agatcccata caatggggta ccttctgggc atccttcagc
      181 cccttgttga atacgcttga ggagagccat ttgactcttt ccacaactat ccaactcaca
      241 acgtggcact ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg cttttggccg
      301 cagaggcacc tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc tacagacgtt
      361 gtttgtcttc aagaagcttc cagaggaact gcttccttca cgacattcaa cagaccttgc
      421 attcctttgg cgagagggga aagaccccta ggaatgctcg tcaagaagac agggccaggt
      481 ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataacat atagacaaac
      541 gcacaccggc cttattccaa gcggcttcgg ccagtaacgt tagggggggg gagggagagg
      601 ggcggatccc gggcccgcgg taccgtcgac tgcagaattc gaagcgctag ctcgagatcc
      661 aaggggcagt gggtcggtgg gccgcggggg cggagcgcaa ggggctcagg agcacggcgg
      721 cgctctctgg cacgacgcga ctcctctgct gcccgctgca gactgaggca gcgctgaggc
      781 tctgccgccg cagagcgtat ggtcgcgcct gcacccccct atcttgcacc tcttgtggtg
      841 cggcccgact ggactcaccg ctgcacggac gc
//
