LOCUS       sequence-329101-        9120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for generation of mammalian cells with inducible expression of 
            dominant negative TRF2 for induction of telomere crisis used 
            together with any PiggyBac transposase plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
  TITLE     ER-directed TREX1 limits cGAS activation at micronuclei.
  JOURNAL   Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037.
  PUBMED    33476576
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037."
COMMENT     Sequence Label: pHP138 Myc-TRF2-deltaB deltaM
FEATURES             Location/Qualifiers
     source          1..9120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    73..91
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    109..127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    145..163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    181..199
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    217..235
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     280..304
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             406..435
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(1707..1726)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1751..1799
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2030..2241
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2254..2522
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2556..3299
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             3300..3353
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3354..3373)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             3402..4205
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3465..3484)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4075..4094
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4298..4419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4335..4354)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4389..4408
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5182..5216
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5707..5723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5707..5723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5720..5742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5731..5747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5755..5761,5762..5779,5780..5785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5800..5821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5938..5955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6109..6697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6189..6208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6868..7659,7660..7728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7491..7510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7729..7833)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7859..8314)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7996..8017)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8208..8227
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8441..8463
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8455..8472
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8456..8472
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8602..8664
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        join(9113..9120,1..371)
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
ORIGIN
        1 tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat agagaacgta
       61 tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc cctatcagtg
      121 atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc tacagtttac
      181 tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat agagaacgta
      241 taagctttag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca
      301 gatcgcctgg agcaattcca caacactttt gtcttatacc aactttccgt accacttcct
      361 accctcgtaa aggtctagag ctagccgcct ggagaaggat ccatggaaca aaagttgatt
      421 tctgaagaag atttggaggc acggctggaa gaggcagtca atcgctgggt gctcaagttc
      481 tacttccacg aggcgctgcg ggcctttcgg ggtagccggt acggggactt cagacagatc
      541 cgggacatca tgcaggcttt gcttgtcagg cccttgggga aggagcacac cgtgtcccga
      601 ttgctgcggg ttatgcagtg tctgtcgcgg attgaagaag gggaaaattt agactgttcc
      661 tttgatatgg aggctgagct cacaccactg gaatcagcta tcaatgtgct ggagatgatt
      721 aaaacggaat ttacactgac agaagcagtg gtcgaatcca gtagaaaact ggtcaaggaa
      781 gctgctgtca ttatttgtat caaaaacaaa gaatttgaaa aggcttcaaa aattttgaaa
      841 aaacatatgt ccaaggaccc cacaactcag aagctgagaa atgatctcct gaatattatt
      901 cgagaaaaga acttggccca tcctgttatc cagaactttt catatgagac cttccagcag
      961 aagatgctgc gcttcctgga gagccacctg gatgacgccg agccctacct cctcacgatg
     1021 gccaaaaagg ctttgaaatc tgagtccgct gcctcaagta cagggaagga agataaacag
     1081 ccagcaccag ggcctgtgga aaagccaccc agagaacccg caaggcagct acggaatcct
     1141 ccaaccacca ttggaatgat gactctgaaa gcagctttca agactctgtc tggtgcacag
     1201 gattctgagg cagcctttgc aaaactggac cagaaggatc tggttcttcc tactcaagct
     1261 ctcccagcat caccagccct caaaaacaag agacccagaa aagatgaaaa cgaaagttca
     1321 gccccggctg acggtgaggg tggctcggaa ctgcagccca agaacaagcg catgacaata
     1381 agcagattgg tcttggagga ggacagccag agtactgagc ccagcgcagg cctcaactcc
     1441 tcccaggagg ccgcttcagc gccaccatcc aagcccaccg ttctcaacca acccctccct
     1501 ggagagaaga atcccaaagt acccaaaggc aagtggaaca gctctaatgg ggttgaagaa
     1561 aaggagactt gggtggaaga ggatgaactg tttcaagttc aggcaccaga tgaagacagt
     1621 acaaccaata taacaaaaaa gcagaagtgg actgtagaat gagaattccg accggttagt
     1681 aatgagttta aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     1741 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
     1801 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     1861 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     1921 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgaaatcg
     1981 gatccagctg aatctaagtg gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     2041 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     2101 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     2161 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     2221 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     2281 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     2341 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     2401 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     2461 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     2521 tctaagctgt gaccggcgcc tactctagag ccgccatgtc tagactggac aagagcaaag
     2581 tcataaactc tgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga
     2641 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
     2701 gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cactcctgcc
     2761 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg
     2821 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
     2881 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
     2941 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac
     3001 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
     3061 ttctgaaaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg
     3121 gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga
     3181 ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg
     3241 accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggg
     3301 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg
     3361 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt catgggatcg gccattgaac
     3421 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     3481 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     3541 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     3601 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     3661 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     3721 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     3781 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     3841 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     3901 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgatgatc
     3961 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4021 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4081 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4141 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     4201 tctgagacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     4261 cctgaaatca acctctggat tacaaaattt gtgggtaaac ttgtttattg cagcttataa
     4321 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4381 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa
     4441 atgatgtcaa ttagtctatc agaagctcat ctggtctccc ttccggggga caagacatcc
     4501 ctgtttaata tttaaacagc agtgttccca aactgggttc ttatatccct tgctctggtc
     4561 aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag
     4621 ccaggtttag ccccggaatt gactggattc cttttttagg gcccattggt atggcttttt
     4681 ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag
     4741 cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg
     4801 cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg
     4861 gagggacgta attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca
     4921 agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat
     4981 cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc
     5041 ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc
     5101 tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga
     5161 gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa
     5221 tctagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct
     5281 ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg
     5341 catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt
     5401 ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc
     5461 aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc
     5521 cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc
     5581 agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct
     5641 ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta
     5701 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5761 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5821 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5881 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5941 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6001 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6061 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6121 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6181 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6241 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6301 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6361 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6421 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6481 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6541 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6601 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6661 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6721 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6781 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6841 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6901 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6961 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7021 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7081 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7141 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7201 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7261 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7321 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7381 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7441 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7501 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7561 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7621 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7681 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7741 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7801 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     7861 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     7921 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     7981 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     8041 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     8101 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     8161 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     8221 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     8281 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     8341 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     8401 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     8461 acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga ggacgggcag
     8521 actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct cgacacgctg
     8581 cagaacacgc agctagatta accctagaaa gataatcata ttgtgacgta cgttaaagat
     8641 aatcatgtgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt ttatttatta
     8701 atttgaatag atattaagtt ttattatatt tacacttaca tactaataat aaattcaaca
     8761 aacaatttat ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa tttcttctat
     8821 aaagtaacaa aacttttatg agggacagcc cccccccaaa gcccccaggg atgtaattac
     8881 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct
     8941 ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac
     9001 gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga
     9061 tacggggaaa aggcctccac ggccactagt ctttcgtctt caagaattcc tcgagtttac
//
