LOCUS       sequence-329109-       10222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal inducible expression of dCas9-FokI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10222)
  TITLE     Burgess Lab Plasmids
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRR2
FEATURES             Location/Qualifiers
     source          1..10222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        78..182
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(183..251,252..1043)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(401..420)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1214..1802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1703..1722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1956..1973
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2090..2111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2126..2131,2132..2149,2150..2156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2164..2180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2169..2191
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2188..2204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2188..2204
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2223..2243
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2225..2243
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2652..2675
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             2713..2727
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             2734..2754
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2791..3378
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             join(3427..3450,3451..3453,3454..5940,5941..5943,
                     5944..7530)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     terminator      7537..7784
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7553..7571)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(7802..7821)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7803..7821)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7831..7848)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7831..7847)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7840..7862)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7988..8443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8075..8094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8285..8306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8546..9220)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(9221..9501)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(9522..9541)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9641..9663
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9701..9719)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(9760..10222,1..41)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 attaaaaaat ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca
       61 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      121 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      181 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      241 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      301 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      361 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      421 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      481 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
      541 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
      601 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
      661 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
      721 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
      781 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
      841 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
      901 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
      961 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1021 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1081 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1141 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1201 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1261 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1321 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1381 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1441 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1501 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1561 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1621 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1681 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1741 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1801 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1861 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1921 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1981 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2041 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2101 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     2161 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     2221 gcgcaattaa ccctcactaa agggaacaaa agctggagct cgtgacagcc ctccgaagga
     2281 agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct
     2341 cgcgccgcac tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga
     2401 ggaaaaattg gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac
     2461 cataggatga taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag
     2521 cgatgatttt tgatctatta acggatatat aaatgcaaaa actgcataac cactttaact
     2581 aatactttca acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac
     2641 aaaaaattgt taatatacct ctatacttta acgtcaagga gaaaaaaccc cggattctag
     2701 aactattaca aggatgacga tgacaagatg gcccccaaga agaagaggaa ggtgggcatt
     2761 caccgcgggg tacctggagg ttctggatcc caactagtca aaagtgaact ggaggagaag
     2821 aaatctgaac ttcgtcataa attgaaatat gtgcctcatg aatatattga attaattgaa
     2881 attgccagaa attccactca ggatagaatt cttgaaatga aggtaatgga attttttatg
     2941 aaagtttatg gatatagagg taaacatttg ggtggatcaa ggaaaccgga cggagcaatt
     3001 tatactgtcg gatctcctat tgattacggt gtgatcgtgg atactaaagc ttatagcgga
     3061 ggttataatc tgccaattgg ccaagcagat gaaatgcaac gatatgtcga agaaaatcaa
     3121 acacgaaaca aacatatcaa ccctaatgaa tggtggaaag tctatccatc ttctgtaacg
     3181 gaatttaagt ttttatttgt gagtggtcac tttaaaggaa actacaaagc tcagcttaca
     3241 cgattaaatc atatcactaa ttgtaatgga gctgttctta gtgtagaaga gcttttaatt
     3301 ggtggagaaa tgattaaagc cggcacatta accttagagg aagtcagacg gaaatttaat
     3361 aacggcgaga taaactttag cggcagcgag actcccggga cctcagagtc cgccacaccc
     3421 gaaagtgata agaaatactc tattggtttg gctatcggta caaactctgt tggttgggct
     3481 gttattactg atgaatacaa ggttccatcc aagaagttca aggttttggg taacactgat
     3541 agacactcca tcaaaaagaa cttgattggt gccttgttgt tcgattctgg tgaaactgct
     3601 gaagctacta gattgaaaag aaccgctaga agaagataca ccagaagaaa gaacagaatc
     3661 tgctacttgc aagaaatctt ctccaacgaa atggccaagg ttgatgattc attcttccac
     3721 agattggaag aatccttctt ggtcgaagaa gataagaagc acgaaagaca tccaatcttc
     3781 ggtaacatcg ttgatgaagt tgcttaccac gaaaagtacc caactatcta ccatttgaga
     3841 aagaagttgg ttgactctac cgataaggct gatttgagat tgatctattt ggctttggcc
     3901 cacatgatta agttcagagg tcatttcttg atcgaaggtg atttgaaccc agataactcc
     3961 gatgttgata agttgttcat ccaattagtc caaacctaca atcaattatt cgaagaaaac
     4021 ccaatcaacg cctctggtgt tgatgctaaa gctattttgt ctgccagatt gtccaagtcc
     4081 agaagattag aaaatttgat cgcccaatta ccaggtgaaa agaagaatgg tttgttcggt
     4141 aatttgattg ccttgtcttt gggtttgact ccaaacttca agtccaattt cgatttggct
     4201 gaagatgcca agttgcaatt atctaaggat acctacgatg acgatttgga taacttgttg
     4261 gctcaaatcg gtgatcaata cgctgatttg tttttggctg ctaagaactt gtccgatgcc
     4321 attttgttgt ccgatatttt gagagtcaac accgaaatta ctaaggctcc attgtctgcc
     4381 tctatgatca aaagatacga tgaacaccac caagacttga ctttgttgaa ggctttggtc
     4441 agacaacaat tacctgaaaa gtacaaagaa attttcttcg atcaatccaa gaacggttac
     4501 gccggttata ttgatggtgg tgcttctcaa gaagaatttt acaagttcat caagccaatc
     4561 ttggaaaaga tggacggtac tgaagaatta ttggtcaagt tgaacagaga agatttgttg
     4621 agaaagcaaa gaaccttcga caacggttct attccacatc aaattcactt gggtgaattg
     4681 cacgcaattt tgagaagaca agaagatttt tatccattct tgaaggacaa cagagaaaag
     4741 atcgaaaaga ttctgacctt cagaatccct tactacgttg gtccattggc tagaggtaat
     4801 tcaagatttg cctggatgac tagaaagtcc gaagaaacta ttactccttg gaacttcgaa
     4861 gaagttgtag ataagggtgc ttctgcccaa tcctttattg aaagaatgac caacttcgac
     4921 aagaacttgc caaacgaaaa ggttttgcca aagcactctt tgttgtacga atacttcacc
     4981 gtctacaacg aattgactaa ggttaagtac gtcaccgaag gtatgagaaa accagctttt
     5041 ttatccggtg aacaaaagaa ggctatcgtc gatttgttgt tcaagaccaa cagaaaggtt
     5101 actgtcaagc aattaaaaga agattacttc aagaaaatcg aatgcttcga ctccgttgaa
     5161 atttctggtg tcgaagatag attcaatgcc tctttaggta cttaccatga cttgttgaaa
     5221 atcatcaagg acaaggattt cttggacaac gaagaaaacg aagatatttt ggaagatatt
     5281 gtcttgacat tgaccttgtt tgaagataga gaaatgattg aagaaagatt gaaaacctac
     5341 gcccacttgt tcgatgataa ggttatgaag caattaaaga gaagaagata cactggttgg
     5401 ggtagattgt ccagaaaatt gattaacggt atcagagaca agcaatccgg taagaccatt
     5461 ttggactttt tgaagtctga tggtttcgct aacagaaact tcatgcaatt aatccacgac
     5521 gattccttga ctttcaaaga agatatacaa aaggcccaag tctctggtca aggtgattct
     5581 ttacatgaac atatcgctaa cttggctggt tctccagcta ttaagaaggg tattttacaa
     5641 accgttaagg tcgttgacga attggtcaaa gttatgggta gacataagcc agaaaacatc
     5701 gttatcgaaa tggctagaga aaatcaaacc acccaaaagg gtcaaaagaa ctccagagaa
     5761 agaatgaaga gaatcgaaga aggtatcaaa gaattgggtt cccaaatttt gaaagaacac
     5821 ccagttgaaa acacccaatt acaaaacgaa aagttgtact tgtactactt gcaaaacggt
     5881 agagatatgt acgttgacca agaattggac atcaacagat tgtctgatta cgatgttgac
     5941 gctatcgttc cacaatcttt tttgaaggat gactccattg acaacaaggt cttgactaga
     6001 tccgataaga atagaggtaa gtccgataac gttccatctg aagaagtcgt taagaaaatg
     6061 aagaactatt ggagacaatt attgaacgcc aagttgatca cccaaagaaa gtttgacaat
     6121 ttgaccaagg ctgaaagagg tggtttgtct gaattggata aggcaggttt catcaaaaga
     6181 caattagtag aaaccagaca aatcaccaag cacgttgctc aaattttgga tagtagaatg
     6241 aacactaagt acgacgaaaa cgacaaattg atcagagaag ttaaggtcat taccttgaag
     6301 tccaagttgg tttccgattt cagaaaggac ttccaattct acaaggtcag agaaatcaac
     6361 aactaccatc atgcacatga tgcttacttg aatgctgttg ttggtactgc cttgattaag
     6421 aagtatccaa agttggaatc cgaatttgtc tacggtgatt acaaggttta cgacgttaga
     6481 aagatgatcg ccaagtccga acaagaaatt ggtaaagcta ctgccaaata cttcttctac
     6541 tccaatatta tgaatttctt taagaccgaa atcactttgg ccaacggtga aattagaaaa
     6601 agaccattga ttgaaactaa tggtgaaaca ggtgaaatcg tttgggataa gggtagagat
     6661 tttgccactg ttagaaaggt attgtccatg ccacaagtaa acatcgtcaa aaagaccgaa
     6721 gttcaaactg gtggtttctc caaagaatcc attttgccta agagaaactc cgataagttg
     6781 atcgctagaa aaaaagactg ggacccaaaa aagtacggtg gttttgattc tccaactgtt
     6841 gcttactctg ttttggttgt tgctaaggtc gaaaagggta agagtaagaa gttgaagtcc
     6901 gtcaaagaat tattaggtat cactatcatg gaaagatcct cattcgaaaa gaatcctatc
     6961 gactttttgg aagccaaggg ttacaaagaa gtcaagaagg acttgatcat taagttgcca
     7021 aagtacagtt tgttcgaatt ggaaaatggt agaaagagaa tgttggcttc tgccggtgaa
     7081 ttacaaaagg gtaatgaatt ggctttgcca tccaagtacg ttaatttctt atacttggcc
     7141 tcccactacg aaaaattgaa aggttctcct gaagataacg aacaaaagca attatttgtc
     7201 gaacaacaca agcactactt ggacgaaatc attgaacaaa tttccgaatt ttccaaaaga
     7261 gtcattttgg ctgacgccaa tttggacaaa gttttgtcag cttacaacaa gcacagagat
     7321 aagccaatta gagaacaagc tgaaaacatc attcacttgt tcactttgac taacttgggt
     7381 gctccagctg cttttaagta tttcgatacc actatcgaca gaaagagata cacctctacc
     7441 aaagaagttt tggacgctac tttgatccac caatctatta ctggtttgta cgaaactaga
     7501 atcgacttgt ctcaattagg tggtgattaa ctcgagtcat gtaattagtt atgtcacgct
     7561 tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg
     7621 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat
     7681 ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa
     7741 ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcggccgg tacccaattc
     7801 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     7861 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     7921 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     7981 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8041 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8101 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8161 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8221 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8281 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     8341 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8401 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc
     8461 tccttacgca tctgtgcggt atttcacacc gcatagatcg gcaagtgcac aaacaatact
     8521 taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt
     8581 tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc
     8641 catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat
     8701 gtaagctttc ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa
     8761 gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg
     8821 aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca
     8881 aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatatcaa
     8941 tgccgtaatc attgaccaga gccaaaacat cctccttagg ttgattacga aacacgccaa
     9001 ccaagtattt cggagtgcct gaactatttt tatatgcttt tacaagactt gaaattttcc
     9061 ttgcaataac cgggtcaatt gttctctttc tattgggcac acatataata cccagcaagt
     9121 cagcatcgga atctagagca cattctgcgg cctctgtgct ctgcaagccg caaactttca
     9181 ccaatggacc agaactacct gtgaaattaa taacagacat actccaagct gcctttgtgt
     9241 gcttaatcac gtatactcac gtgctcaata gtcaccaatg ccctccctct tggccctctc
     9301 cttttctttt ttcgaccgaa ttaattctta atcggcaaaa aaagaaaagc tccggatcaa
     9361 gattgtacgt aaggtgacaa gctatttttc aataaagaat atcttccact actgccatct
     9421 ggcgtcataa ctgcaaagta cacatatatt acgatgctgt tctattaaat gcttcctata
     9481 ttatatatat agtaatgtcg tgatctatgg tgcactctca gtacaatctg ctctgatgcc
     9541 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     9601 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     9661 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     9721 ttataggtta atgtcatgat aataatggtt tcttagacgg atcgcttgcc tgtaacttac
     9781 acgcgcctcg tatcttttaa tgatggaata atttgggaat ttactctgtg tttatttatt
     9841 tttatgtttt gtatttggat tttagaaagt aaataaagaa ggtagaagag ttacggaatg
     9901 aagaaaaaaa aataaacaaa ggtttaaaaa atttcaacaa aaagcgtact ttacatatat
     9961 atttattaga caagaaaagc agattaaata gatatacatt cgattaacga taagtaaaat
    10021 gtaaaatcac aggattttcg tgtgtggtct tctacacaga caagatgaaa caattcggca
    10081 ttaatacctg agagcaggaa gagcaagata aaaggtagta tttgttggcg atccccctag
    10141 agtcttttac atcttcggaa aacaaaaact attttttctt taatttcttt ttttactttc
    10201 tatttttaat ttatatattt at
//
