LOCUS       sequence-329143-       17206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ubiquitous promoter followed by a lacZ to GFP lineage tracing line. 
            Cryst:venusGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17206)
  AUTHORS   Di Donato V, De Santis F, Auer TO, Testa N, Sanchez-Iranzo H, 
            Mercader N, Concordet JP, Del Bene F
  TITLE     2C-Cas9: a versatile tool for clonal analysis of gene function.
  JOURNAL   Genome Res. 2016 Mar 8.
  PUBMED    26957310
REFERENCE   2  (bases 1 to 17206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2016 Mar 8."
COMMENT     Sequence Label: Hulk-ubi-lacZ-to-Green
FEATURES             Location/Qualifiers
     source          1..17206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     4687..4706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(4751..4784)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4863..7904
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQ"
     primer_bind     complement(4865..4887)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7929..8732
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7992..8011)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8602..8621
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          8832..8897
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    9057..9191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    9294..9428
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    9531..9665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(9698..9731)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    9775..9799
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(9805..9807,9808..9810,9811..10521)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9805..9807,9808..9810,9811..10521)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(10583..10607)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    10609..10743
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(10806..10826)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10834..10850)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    10866..11000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    11193..11327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(11378..12091,12092..12094,12095..12097))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(12841..12852)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        complement(14348..14366)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(14736..14923,14924..14935)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(14969..14985)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(15022..15040)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(15061..15077)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15074..15096)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15085..15101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15109..15115,15116..15133,15134..15139))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15154..15175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15292..15309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15463..16051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15543..15562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16222..17013,17014..17082))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16845..16864
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17083..17187)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttaaaact
     1141 cgagaccagc aaagttctag aatttgtcga aacatttatg ttatatattt cctgaaaaaa
     1201 attctgagta agttcttaag tgttattgcc agcaacataa acaacagacg gcaaaatgaa
     1261 taaatgataa caaagcagta ggcttaaata aacctaattt ttataggctg ttctctacaa
     1321 ccctcaaaca gtgattagtt ttgtacttat aaacttgccc tttcattcat atttcaagaa
     1381 aattggttca gaagatctgg atattctagc agttgttcaa gctcatggag ggatcagtga
     1441 cctgattcca caatgactag gcctaatcca gaaattagat gactgtcaac ataaaaaggc
     1501 acagcactca ctagctgccc tatatatttt attatatttt acatatatta ttttatttat
     1561 ttagctctga gtgctgtact ttctggttaa agaaaactgc ttacaacagc taacctgtac
     1621 tacctcaggc tcagggaatt tggaacaggt ttgtctggtt tgtttcttta accatgcatg
     1681 cttgttttca actatggcaa cacagtcaca tgggacatta cagaaatgat ttgtcgatga
     1741 catgcgactt ttctttaata aagcgcaaag atcccaaaaa gcaaactttt aacaaaaatc
     1801 atataattat attttcaatc cagctttgta gcaactttgt gctgctgttc actcagcaac
     1861 agatagtcag tataaggtca gtgtgtctca aagcagtgcc atctgtttca cacattgcgt
     1921 tctatatata agtgtgctgg ttgacacgac actgtataag gcctaggcta aaacacaaac
     1981 aatgtagaat gacactgtgt tttttttgta aacaaatgtt gtttttggtt aaacatcttt
     2041 gtgaaaacat cctcctgtca tgtatttgct atattcaaat gttaaacccg tgcagaatag
     2101 aacatataca aaaaaaaaca acacaacaca tttttaaaca ttattaaata tcaagtattg
     2161 ctggcagttc tgtttctgtt ttacagtacc ctttgccaca gttctccgct tttcctggtc
     2221 cagattccac aagtctgatt caccaatagc aaagcgaata aacaaccaaa gcagccaatc
     2281 actgcttgta gactgtcctg cgagaccggc ccattccagc acattctgga aacttccttt
     2341 atatgataat tataaataca tttaaattat tgatacaaaa catgtaattc ctagaacata
     2401 accatagcaa tcattagttt tcagggtaat tatgtatttt taggatttga ctgcggaaag
     2461 atctggtcat gtgacgtctc atgaacgtca cggccctggg tttctataaa tacagtagga
     2521 ctctcgacca tcggcagatt tttcgaagaa gaagatcagt ttcaggagcc gtactgttcc
     2581 gtttcaacgc aaatattaac ggtaagagcg aatttcctag tttgttttca tgccattctt
     2641 taaaaccata gcgtattact ttaattatag taaactttcg ctttctttat tacaagagac
     2701 gttttgtgtt gattctccgc ggacattttc ggtcagacaa tcagaaaatg accgcggagg
     2761 accagtaact tgcattacac gtaagttaaa tcttcgtgta ttaaaatggt taggttgtta
     2821 acgtcaaata ggttaccgtg tttgcgtgtg atcaggttgg ttttgttaga tttttgtcag
     2881 tatttttaat ttatttgttt tagtttattt attttttttg ctgaatcata gtttgtgaac
     2941 aaagaacccg gatgttacat acagtacagc cgccatgtta cagagagtta taacttaatc
     3001 attttaaaaa taattttgcc ttacttttag tttgtcatgt tgagaaatga ggaaatgtta
     3061 aaatgaggaa atatccaatt aatttaatat atcaaaataa tccatgatta caatgcactg
     3121 aactggagaa aattaagatg ttttctagtg tcatgaaaca aatgtaagag atgtacattg
     3181 tagatgtttt atgtcaagaa ttggctagtt gatgcagcat actggcgata ctcagttgta
     3241 ataacagtaa cgttacatgt taatagacta ctgagtatgc tgttctgtct atgtatgctc
     3301 tgtaagctac gagaaggact tttttaaaca gtaaagggtg caatattttt acaaattgaa
     3361 ttaaataaag gctgtctatt aagtaatatg cttgatattt ttcttacttg atcggaaata
     3421 agaaaaaata taaacgttgt tgctctaaaa atcctagttc agtttagcca accacaaata
     3481 cctttttgtt cctccaacag tttttttttc ttctctataa tatttggcag tctatagtac
     3541 tccaaatgtt tccccacagt ctaactaatt ggtacagcca aaatcatgac acttattgca
     3601 ataataattt tggttcattg gcattgttga tagcctgtgc cactaatatg gtcgattgat
     3661 catgcttcag gaagaaaact atattgtttg atgtaagatt attaaatctt cacctgcttc
     3721 cattacaaac tattcccatc ttattgaatt ctggtatgtc ttaaaggatt agttcacttc
     3781 ccaaatcaaa atttacttag ccttttttca tccatgatcc ctttttttca tcattaatga
     3841 agaaattgtt tttgaaaaag tttcaagatt tttttctcta tattgtggag cttgttagtt
     3901 taaaattcca aaatgcaata tgtggcttca aatggttcta aatgatccca gtcaaggaat
     3961 aacagtctta tctaatgaaa ccattagacc tttaaaaata aaaaaaataa aagtatttat
     4021 ttttaaatga ctgagtgatt aagttgaatt tcagcgtttc cttactgtgt agaagtcctt
     4081 ccttactggc cccacccttt ggttctctgc caatctgcta cctaatgtaa tgttgtggaa
     4141 cattattatt ctttatttct taatttttta ttttttattt taaaacaatg taaactgcac
     4201 agatgtgcag tttgtttaaa atggccaatg ctttggaaat gcatgacata attagatttc
     4261 atgatgcaca aagccaaatc tcagagcttg tgcaaaatga gctatcattt cactaggtaa
     4321 gaccctaaat tttcatatag gatcatttgg acaattttgc tgcaggtaaa atgcattcta
     4381 tagtccactg tcagccattg ttttggatag tatttatttt tctctacaag tatagtcaat
     4441 agttttctat tattttaaag gtttgtaaca tttaagggtg accaaatgca aagtaaaatt
     4501 tcattttcgg gtgaactatc tcgtttaaca tgggagaagt gcaaaacata cattattggc
     4561 tagaacattg tagtattttt taaatggaaa tgtgtgattg ctaatcttac tttgaatttg
     4621 tttacaggga tcctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     4681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcctcgatc
     4741 gagggaccta ataacttcgt atagcataca ttatacgaag ttatattaag ggttccgcaa
     4801 gcttcctaga ctagtcgacg gtatcgataa gcagcttgat gatctgtgac atggcggatc
     4861 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4921 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4981 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
     5041 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
     5101 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta
     5161 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
     5221 atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
     5281 cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc
     5341 cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
     5401 tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
     5461 ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
     5521 ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
     5581 gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
     5641 ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc
     5701 gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
     5761 tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
     5821 gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
     5881 cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
     5941 atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
     6001 gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
     6061 atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
     6121 atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
     6181 gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc
     6241 acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
     6301 gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg
     6361 aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg
     6421 gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg
     6481 gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct
     6541 gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt
     6601 acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct
     6661 ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc
     6721 agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
     6781 ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag
     6841 tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc
     6901 cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat
     6961 ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg
     7021 tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt
     7081 gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga
     7141 tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
     7201 cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg
     7261 aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag
     7321 atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa
     7381 ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
     7441 ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc
     7501 tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
     7561 accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
     7621 cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
     7681 cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga
     7741 tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
     7801 gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc
     7861 agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaggggatc ccccgggctg
     7921 cagccaatat gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt
     7981 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     8041 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     8101 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     8161 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     8221 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     8281 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     8341 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     8401 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     8461 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     8521 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     8581 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     8641 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     8701 ccttctatcg ccttcttgac gagttcttct gaggggatca attctctagg cttgggatct
     8761 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
     8821 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
     8881 ttgtttgtgt attttagatt cacagtccca aggctcattt caggcccctc agtcctcaca
     8941 gtctgttcat gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     9001 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     9061 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9121 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9181 atgtctggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     9241 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     9301 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9361 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9421 tctggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     9481 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     9541 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9601 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9661 ggatccacta gttctagcta gtctaggtcg atgcaggata acttcgtata gcatacatta
     9721 tacgaagtta tagatcttgg gtacccgctc gaatccttta agggcgaatt cgacccaagt
     9781 ttgtacaaaa aagcaggctg gaccatggtg agcaagggcg aggagctgtt caccggggtg
     9841 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     9901 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     9961 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
    10021 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
    10081 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
    10141 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
    10201 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
    10261 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
    10321 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
    10381 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
    10441 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
    10501 ggcatggacg agctgtacaa gggcggtgga agatctggga attcaaggcc tctcgagcct
    10561 ctagattctg cagccctata gcacccagct ttcttgtaca aagtggggga tccagacatg
    10621 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
    10681 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
    10741 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
    10801 ttttccaact ttattataca tagttgataa ttcactggcc gtcgttttac ggtaccatcg
    10861 atgatgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
    10921 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
    10981 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
    11041 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
    11101 atgatcctct agatcagatc ggccgcgaat taaaaaacct cccacacctc cccctgaacc
    11161 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
    11221 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    11281 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctac gtaatacgac
    11341 tcactatagt tctagaggct cgagaggcct tgaattctta cttgtacagc tcgtccatgc
    11401 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
    11461 tggggtcttt gctcagggcg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg
    11521 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ccgccgtcct
    11581 cgatgttgtg gcggatcttg aagttggcct tgatgccgtt cttctgcttg tcggcggtga
    11641 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
    11701 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
    11761 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
    11821 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg
    11881 cgaagcactg caggccgtag cccagggtgg tcacgagggt gggccagggc acgggcagct
    11941 tgccggtggt gcagatcagc ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
    12001 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
    12061 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggatcccccg caagtctgac
    12121 tgagtggaag tttcagttca atggaaggga gaaaagagcc agcctttata tatgtcatgt
    12181 attgctgctg tctgcatgcg atgaacctag agcttacttt ttctgtcaag taaggcaaaa
    12241 gcagagaacc tttgtcttgc attgtgtttg atgttgtgct gtgacagtca tttcttagtt
    12301 catgctggta cttttgttcc atagctttac ccaaagagtt atccagcatt cccagttatt
    12361 ccaaaaactc aatgtcacaa attttccagt ccattgctta gcagcgttga ccccggagac
    12421 cctgatgtgg taatttacag tgcaattcag ttgctggtcc aaacactttg taacagttta
    12481 aagaattgtg gttactagta aatccttaat ttggcatcat ctaggtgtca ttctttgcca
    12541 cttttgaatc acacaaatga accaaacaat ggttagctag catcaaacat ttaccaaata
    12601 atctcatcag tgtcacccca atgtttaaga agccacattt tatgtcccca gatatattaa
    12661 tattatttta tatataattt aaaatagtaa tgtggtgtta ctgtatttgt catataacgc
    12721 aatatataca gtatgtcttt atctgtatca attttgtggt cacaccatca ataaactcca
    12781 gtttaattat ttaaaattgg gtagtgataa ccatataaag gaacagtggc tagttccatg
    12841 ttgtagctcc caacctgctt tcaggtgatc catgcagtgt cgtataaaaa ggtaacaatt
    12901 tttaaccttg gaaagcaagt acacttaagg tccccataga cgcgacgatt cttcttgccg
    12961 aacgaccgat tttagggaag cccgaccaat ccttcgaaat tatcgtgcgg ttagtggtat
    13021 tcgaacgatc gtacatctta cgatttttcg gccgacatct gtcgggaaat tgatcggcca
    13081 ggtcaaaaaa tctttgtcgg ccccagtgca atctctctat gtttgcaggg ccaagcaggc
    13141 agctcccctt tgttttcctg gtaaattggt ctttttagtt gatggtagat tcgtacgatc
    13201 gtacgatcgt tctgagaaga tcgtggtctc acgatcagga tctgatcttt taaaaatctc
    13261 aacatctatg gccagcttta ctttctgcta gaatgtttgt gttttattgg ccagaccagg
    13321 ggtgactttg agacagttgg tcagcttgga tatattgcaa tatatggaca aataatccca
    13381 gacccactgg tacagtgttc cctatattaa agaatggggg ggtatgataa tttcaatctg
    13441 gaaatactaa aggtgggaat gttactaaaa aaatcacaag tgatgttaaa aatagaggta
    13501 tactcatttg ctggcgaata ttacatgact aagttaagca tctgctctgg agtttttaaa
    13561 tattttgttg caaaatttta ttacatacac taggaatgtt cacatatgcc atttggtcac
    13621 aaaaggacgc aaatatggtc gctaacattc acattggtca ttgtcaaatg tttaggtgct
    13681 aatgaagcat atctcattcc cagtgcttga attggagtaa aaaaagtgga gggatgctct
    13741 gtgtggtgca tccctcccat aaccatattt acatatgtga cctataggga ccccaaaata
    13801 aaaattgtgc ttatgacttt tcttggctac caattcagct actgcaaata aagcaccttt
    13861 tctgtgtatt gtgtcataac atagcacatc cccatcatat tctatattac ccaagattat
    13921 ttcatataaa gcattttctc actattcctt aatctgctgc tgtttaatac cttcattttt
    13981 tcttctctcc ttttctctat aaccctctct gatgcctcca ccttctttat ctgtttaaca
    14041 cttctctatc ttttcttctt ctctcccagc aattttttct ctcctttaat cccctatata
    14101 tttaactata ttatatttta cctctgcatt gtatttgcat gcagaagtga agaggagcaa
    14161 aagacagttc cgggattcaa aaaacttgca tccacactga ggggatgcta tttcacagaa
    14221 ataaaaaagt gaggggatgg catccctccc caattccagc actgctagtt gacaattctg
    14281 cattctctcc agcttattta tatctttctt atggactcga gggggggccc ggtaccagct
    14341 tttgttccct ttagtgaggg ttaattgcgc gccccaagct tggatctcga ctctagttct
    14401 agagcggccg ccacctctgc gaagatacgg ccacgggtgc tcttgatcct gtggctgatt
    14461 ttggactgtg ctgctcgcag ctgctgatga atcacatact tcctccattt tcttccactg
    14521 attgactgtt ataatttccc taatttccag gtcaaggtgc tgtgcattgt ggtaatagat
    14581 gtgacatgac gtcacttcca aaggaccaat gaacatgtct gaccaatttc atataatgtg
    14641 aaaacgattt tcataggcag aataaataac atttaaatta aactgggcat cagcgcaatt
    14701 caattggttt ggtaatagca agggaaaata gaatgaagtg atctccaaaa aataagtact
    14761 ttttgactgt aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa
    14821 ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa
    14881 aataatactt aagtacagta atcaagtaaa attactcaag tactttacac ctctggttct
    14941 tgacccccta ccttcagcaa gcccagcaga tccactagtt ctagagcggc cgccaccgcg
    15001 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
    15061 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    15121 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    15181 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    15241 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    15301 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    15361 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    15421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    15481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    15541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    15601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    15661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    15721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    15781 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    15841 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    15901 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    15961 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    16021 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    16081 tgacgctcag tggaacgaaa actcacgtta aggcattttg gtcatgagat tatcaaaaag
    16141 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    16201 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    16261 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    16321 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    16381 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    16441 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    16501 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    16561 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    16621 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    16681 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    16741 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    16801 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    16861 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    16921 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    16981 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    17041 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    17101 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    17161 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtg
//
