LOCUS       sequence-329145-       11373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For inducing predictable deletions in protoplasts and wheat plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Wang S, Zong Y, Lin Q, Zhang H, Chai Z, Zhang D, Chen K, Qiu JL, Gao
            C
  TITLE     Precise, predictable multi-nucleotide deletions in rice and wheat 
            using APOBEC-Cas9.
  JOURNAL   Nat Biotechnol. 2020 Dec;38(12):1460-1465. doi: 
            10.1038/s41587-020-0566-4. Epub 2020 Jun 29.
  PUBMED    32601432
REFERENCE   2  (bases 1 to 11373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0566-4"; journalName: "Nat Biotechnol"; date: 
            "2020-12"; volume: "38"; issue: "12"; pages: "1460-1465"
COMMENT     Sequence Label: pJIT163-eAFID-3
FEATURES             Location/Qualifiers
     source          1..11373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1458..5558
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1458..5558
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5559..5606
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6312..6332
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6348..6404
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7191..7211
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7269..7445
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7999..8015)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7999..8015)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8012..8034)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8023..8039
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8047..8053,8054..8071,8072..8077))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8092..8113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8230..8247)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8401..8989)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8481..8500)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9160..9951,9952..10020))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9783..9802
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10021..10125)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt cgttgcatag
       61 ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt gtcgggtcat
      121 cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc ggtcgttcta
      181 gatcggagta gaattaattc tgtttcaaac tacctggtgg atttattaat tttggatctg
      241 tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg aaatatcgat
      301 ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttg
      361 ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc tagatcggag
      421 tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta tgtgtgtgtc
      481 atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg ataggtatac
      541 atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat ctattcatat
      601 gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa ttattttgat
      661 cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt tagccctgcc
      721 ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg
      781 gtgttacttc tgcaaagctt gtcgacggat ccatggagat cctcaggtac ctcatggacc
      841 cagacacctt caccttcaac ttcaacaacg acccactcgt gctcaggagg aggcaaacct
      901 acctctgcta cgaggtggag aggctcgata atggcacctg ggtgctcatg gaccaacaca
      961 tgggcttcct ctgcaacgag gccaagaatc tcctctgcgg cttctatggc aggcatgccg
     1021 aactcaggtt tctcgacctc gtgccatccc ttcaactcga cccagcccaa atctacaggg
     1081 tgacctggtt catctcctgg tccccatgtt tttcctgggg ctgtgccgga gaagtgaggg
     1141 cctttctcca agagaacacc cacgtgaggc tcaggatttt tgccgccagg atctacgatt
     1201 acgacccact ctacaaggag gccctccaaa tgcttaggga tgccggagca caagtgtcca
     1261 tcatgaccta cgacgagttc gagtactgct gggacacctt cgtctacagg caaggctgcc
     1321 cattccaacc atgggacggc ctcgaagaac attcccaagc cctctcagga aggcttaggg
     1381 ctatcctcca aaaccaaggc aactccggct ccgaaacccc aggcacttcc gaatccgcca
     1441 ctccagaatc ccttaaggac aagaagtact cgatcggcct cgacatcggg acgaactcag
     1501 ttggctgggc cgtgatcacc gacgagtaca aggtgccctc taagaagttc aaggtcctgg
     1561 ggaacaccga ccgccattcc atcaagaaga acctcatcgg cgctctcctg ttcgacagcg
     1621 gggagaccgc tgaggctacg aggctcaaga gaaccgctag gcgccggtac acgagaagga
     1681 agaacaggat ctgctacctc caagagattt tctccaacga gatggccaag gttgacgatt
     1741 cattcttcca ccgcctggag gagtctttcc tcgtggagga ggataagaag cacgagcggc
     1801 atcccatctt cggcaacatc gtggacgagg ttgcctacca cgagaagtac cctacgatct
     1861 accatctgcg gaagaagctc gtggactcca ccgataaggc ggacctcaga ctgatctacc
     1921 tcgctctggc ccacatgatc aagttccgcg gccatttcct gatcgagggg gatctcaacc
     1981 cagacaacag cgatgttgac aagctgttca tccaactcgt gcagacctac aaccaactct
     2041 tcgaggagaa cccgatcaac gcctctggcg tggacgcgaa ggctatcctg tccgcgaggc
     2101 tctcgaagtc caggaggctg gagaacctga tcgctcagct cccaggcgag aagaagaacg
     2161 gcctgttcgg gaacctcatc gctctcagcc tggggctcac cccgaacttc aagtcgaact
     2221 tcgatctcgc tgaggacgcc aagctgcaac tctccaagga cacctacgac gatgacctcg
     2281 ataacctcct ggcccagatc ggcgatcaat acgcggacct gttcctcgct gccaagaacc
     2341 tgtcggacgc catcctcctg tcagatatcc tccgcgtgaa caccgagatc acgaaggctc
     2401 cactctctgc ctccatgatc aagcgctacg acgagcacca tcaggatctg accctcctga
     2461 aggcgctggt ccgccaacag ctcccggaga agtacaagga gattttcttc gatcagtcga
     2521 agaacggcta cgctgggtac atcgacggcg gggcctcaca agaggagttc tacaagttca
     2581 tcaagccaat cctggagaag atggacggca cggaggagct cctggtgaag ctcaacaggg
     2641 aggacctcct gcggaagcag agaaccttcg ataacggcag catcccccac caaatccatc
     2701 tcggggagct gcacgccatc ctgagaaggc aagaggactt ctaccctttc ctcaaggata
     2761 accgggagaa gatcgagaag atcctgacct tcagaatccc atactacgtc ggccctctcg
     2821 cgcgggggaa ctcaagattc gcttggatga cccgcaagtc tgaggagacc atcacgccgt
     2881 ggaacttcga ggaggtggtg gacaagggcg ctagcgctca gtcgttcatc gagaggatga
     2941 ccaacttcga caagaacctg cccaacgaga aggtgctccc taagcactcg ctcctgtacg
     3001 agtacttcac cgtctacaac gagctcacga aggtgaagta cgtcaccgag ggcatgcgca
     3061 agccagcgtt cctgtccggg gagcagaaga aggctatcgt ggacctcctg ttcaagacca
     3121 accggaaggt cacggttaag caactcaagg aggactactt caagaagatc gagtgcttcg
     3181 attcggtcga gatcagcggc gttgaggacc gcttcaacgc cagcctcggg acctaccacg
     3241 atctcctgaa gatcatcaag gataaggact tcctggacaa cgaggagaac gaggatatcc
     3301 tggaggacat cgtgctgacc ctcacgctgt tcgaggacag ggagatgatc gaggagcgcc
     3361 tgaagacgta cgcccatctc ttcgatgaca aggtcatgaa gcaactcaag cgccggagat
     3421 acaccggctg ggggaggctg tcccgcaagc tcatcaacgg catccgggac aagcagtccg
     3481 ggaagaccat cctcgacttc ctcaagagcg atggcttcgc caacaggaac ttcatgcaac
     3541 tgatccacga tgacagcctc accttcaagg aggatatcca aaaggctcaa gtgagcggcc
     3601 agggggactc gctgcacgag catatcgcga acctcgctgg ctcccccgcg atcaagaagg
     3661 gcatcctcca gaccgtgaag gttgtggacg agctcgtgaa ggtcatgggc cggcacaagc
     3721 ctgagaacat cgtcatcgag atggccagag agaaccaaac cacgcagaag gggcaaaaga
     3781 actctaggga gcgcatgaag cgcatcgagg agggcatcaa ggagctgggg tcccaaatcc
     3841 tcaaggagca cccagtggag aacacccaac tgcagaacga gaagctctac ctgtactacc
     3901 tccagaacgg cagggatatg tacgtggacc aagagctgga tatcaaccgc ctcagcgatt
     3961 acgacgtcga tcatatcgtt ccccagtctt tcctgaagga tgactccatc gacaacaagg
     4021 tcctcaccag gtcggacaag aaccgcggca agtcagataa cgttccatct gaggaggtcg
     4081 ttaagaagat gaagaactac tggaggcagc tcctgaacgc caagctgatc acgcaaagga
     4141 agttcgacaa cctcaccaag gctgagagag gcgggctctc agagctggac aaggccggct
     4201 tcatcaagcg gcagctggtc gagaccagac aaatcacgaa gcacgttgcg caaatcctcg
     4261 actctcggat gaacacgaag tacgatgaga acgacaagct gatcagggag gttaaggtga
     4321 tcaccctgaa gtctaagctc gtctccgact tcaggaagga tttccagttc tacaaggttc
     4381 gcgagatcaa caactaccac catgcccatg acgcttacct caacgctgtg gtcggcaccg
     4441 ctctgatcaa gaagtaccca aagctggagt ccgagttcgt gtacggggac tacaaggttt
     4501 acgatgtgcg caagatgatc gccaagtcgg agcaagagat cggcaaggct accgccaagt
     4561 acttcttcta ctcaaacatc atgaacttct tcaagaccga gatcacgctg gccaacggcg
     4621 agatccggaa gagaccgctc atcgagacca acggcgagac gggggagatc gtgtgggaca
     4681 agggcaggga tttcgcgacc gtccgcaagg ttctctccat gccccaggtg aacatcgtca
     4741 agaagaccga ggtccaaacg ggcgggttct caaaggagtc tatcctgcct aagcggaaca
     4801 gcgacaagct catcgccaga aagaaggact gggacccaaa gaagtacggc gggttcgaca
     4861 gccctaccgt ggcctactcg gtcctggttg tggcgaaggt tgagaagggc aagtccaaga
     4921 agctcaagag cgtgaaggag ctcctgggga tcaccatcat ggagaggtcc agcttcgaga
     4981 agaacccaat cgacttcctg gaggccaagg gctacaagga ggtgaagaag gacctgatca
     5041 tcaagctccc gaagtactct ctcttcgagc tggagaacgg caggaagaga atgctggctt
     5101 ccgctggcga gctccagaag gggaacgagc tcgcgctgcc aagcaagtac gtgaacttcc
     5161 tctacctggc ttcccactac gagaagctca agggcagccc ggaggacaac gagcaaaagc
     5221 agctgttcgt cgagcagcac aagcattacc tcgacgagat catcgagcaa atctccgagt
     5281 tcagcaagcg cgtgatcctc gccgacgcga acctggataa ggtcctctcc gcctacaaca
     5341 agcaccggga caagcccatc agagagcaag cggagaacat catccatctc ttcaccctga
     5401 cgaacctcgg cgctcctgct gctttcaagt acttcgacac cacgatcgat cggaagagat
     5461 acacctccac gaaggaggtc ctggacgcga ccctcatcca ccagtcgatc accggcctgt
     5521 acgagacgag gatcgacctc tcacaactcg gcggggataa gagacccgca gcaaccaaga
     5581 aggcagggca agcaaagaag aagaagacgc gtgactccgg cggcagcgcc aacgagctga
     5641 cctggcacga cgtgctcgcc gaggagaagc aacagccata cttcctgaac accctccaaa
     5701 ccgtggcttc cgagaggcag agcggcgtca ccatctaccc accacaaaag gacgtgttca
     5761 acgccttccg cttcaccgag ctgggcgacg tcaaggtggt catcctcggc caagacccat
     5821 accacggccc aggccaggct cacggcctcg ccttctccgt caggccaggc atcgccatcc
     5881 caccaagcct cctgaacatg tacaaggagc tggagaacac catcccaggc ttcaccaggc
     5941 caaaccacgg ctacctcgag tcctgggcta ggcaaggcgt gctcctgctc aacaccgtgc
     6001 tgaccgtcag ggctggccag gctcactccc acgccagcct gggctgggag acgttcaccg
     6061 acaaggtcat ctccctcatc aaccagcaca gggagggcgt ggtgttcctc ctctggggca
     6121 gccacgccca aaagaagggc gccatcatcg acaagcagag gcaccacgtg ctgaaggccc
     6181 cacacccgtc cccactctcc gctcacaggg gcttcttcgg ctgcaaccac ttcgtgctgg
     6241 ccaaccaatg gctcgagcag aggggcgaga cgccaatcga ctggatgcca gtcctcccag
     6301 ccgagagcga gccaaagaag aagcggaagg tgtcggccgg ctccggcgct acgaacttct
     6361 ccctcctgaa gcaggctggc gacgtggagg agaacccagg cccgccggag ggcccagaga
     6421 tcaggcgcgc tgctgacaac ctcgaggctg ccatcaaggg caagccactg accgacgtgt
     6481 ggttcgcctt cccacaactc aagccatacc aatcccagct gatcggccag cacgtgaccc
     6541 acgtggagac gaggggcaag gccctcctga cccacttctc caacgacctg accctctaca
     6601 gccacaacca actctacggc gtgtggcgcg tggtggacac cggcgaggag ccacaaacca
     6661 cccgcgtgct gagggtcaag ctccagaccg ccgacaagac catcctcctg tactccgcca
     6721 gcgacatcga gatgctgacc ccagagcaac tcaccaccca cccattcctc cagcgcgtgg
     6781 gcccagacgt cctggaccca aacctcaccc cagaggtggt caaggagagg ctcctgagcc
     6841 caaggttccg caacaggcaa ttcgccggcc tcctgctgga ccaggccttc ctggctggcc
     6901 tcggcaacta cctgcgcgtg gagatcctct ggcaagtcgg cctgaccggc aaccacaagg
     6961 ccaaggacct caacgctgct cagctggacg ccctcgctca cgccctgctc gagatcccac
     7021 gcttctccta cgccaccagg ggccaagtgg acgagaacaa gcaccacggc gccctcttcc
     7081 gcttcaaggt gttccaccgc gacggcgagc catgcgagag gtgcggcagc atcatcgaaa
     7141 agaccaccct gtccagcagg ccattctact ggtgcccagg ctgccagcac ccaaagaaga
     7201 agcggaaggt gtaggaattc ggtacgctga aatcaccagt ctctctctac aaatctatct
     7261 ctctctattt tctccataaa taatgtgtga gtagtttccc gataagggaa attagggttc
     7321 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     7381 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc
     7441 agatctccta aagtccctat agatctttgt cgtgaatata aaccagacac gagacgacta
     7501 aacctggagc ccagacgccg ttcgaagcta gaagtaccgc ttaggcagga ggccgttagg
     7561 gaaaagatgc taaggcaggg ttggttacgt tgactccccc gtaggtttgg tttaaatatg
     7621 atgaagtgga cggaaggaag gaggaagaca aggaaggata aggttgcagg ccctgtgcaa
     7681 ggtaagaaga tggaaatttg atagaggtac gctactatac ttatactata cgctaaggga
     7741 atgcttgtat ttatacccta taccccctaa taacccctta tcaatttaag aaataatccg
     7801 cataagcccc cgcttaaaaa ttggtatcag agccatgaat aggtctatga ccaaaactca
     7861 agaggataaa acctcaccaa aatacgaaag agttcttaac tctaaagata aaagatcttt
     7921 caagatcaaa actagttccc tcacaccggt gacggggatc gcatgcgata tctcgagatc
     7981 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8041 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8101 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8161 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8221 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8281 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8341 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8401 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8461 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8521 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8581 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8641 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8701 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8761 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8821 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8881 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8941 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9001 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9061 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9121 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9181 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9241 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9301 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9361 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9421 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9481 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9541 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9601 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9661 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9721 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9781 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9841 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9901 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9961 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10021 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10081 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10141 agtgccacct gccagtgcca agctaattcg agctcggtac ctgacccggt cgtgcccctc
    10201 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
    10261 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
    10321 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
    10381 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
    10441 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
    10501 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
    10561 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
    10621 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
    10681 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
    10741 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
    10801 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
    10861 cctctcgatc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc atccagaaat
    10921 tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct cctctcacgg
    10981 caccggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc
    11041 cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc
    11101 acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg
    11161 ccgctcgtcc tccccccccc ccctctctac cttctctaga tcggcgttcc ggtccatggt
    11221 tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt gtgttagatc
    11281 cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc agacacgttc tgattgctaa
    11341 cttgccagtg tttctctttg gggaatcctg gga
//
