LOCUS       sequence-329177-        1562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Chu D, Nguyen A, Smith SS, Vavrusova Z, Schneider RA
  TITLE     Stable integration of an optimized inducible promoter system enables
            spatiotemporal control of gene expression throughout avian 
            development.
  JOURNAL   Biol Open. 2020 Oct 6;9(10). pii: bio.055343. doi: 
            10.1242/bio.055343.
  PUBMED    32917762
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2020-10-6"; volume: "9(10). pii: bio.055343. doi"; 
            pages: " 10.1242/bio.055343"
COMMENT     Sequence Label: pNano
FEATURES             Location/Qualifiers
     source          1..1562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..84
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(85..153,154..942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(303..322)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      971..1559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1460..1479
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaattcgata tctcgagaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
       61 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      121 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      181 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      241 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      301 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      361 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      421 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      481 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      541 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      601 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      661 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      721 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      781 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      841 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
      901 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtacccgta gaaaagatca
      961 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1021 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1081 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1141 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1201 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1261 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1321 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1381 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1441 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1501 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1561 ac
//
