LOCUS       sequence-329219-       13160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a 
            photoswitchable tethered ligand .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Durand-de Cuttoli R, Mondoloni S, Marti F, Lemoine D, Nguyen C, 
            Naude J, d'Izarny-Gargas T, Pons S, Maskos U, Trauner D, Kramer RH, 
            Faure P, Mourot A
  TITLE     Manipulating midbrain dopamine neurons and reward-related behaviors 
            with light-controllable nicotinic acetylcholine receptors.
  JOURNAL   Elife. 2018 Sep 4;7. pii: 37487. doi: 10.7554/eLife.37487.
  PUBMED    30176987
REFERENCE   2  (bases 1 to 13160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-09-4"; volume: "7. pii: 37487. doi"; pages: " 
            10.7554/eLife.37487"
COMMENT     Sequence Label: pLenti-pGK-nAChRb2(E61C)-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..13160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     131..150
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      227..236
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1748..1757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(1811..2385,2386..2388,2389..2397)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1978..1995)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2205..2224
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2398..2400,2401..2403,2404..3117)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2398..2400,2401..2403,2404..3114)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2443..2464)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2704..2723)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3051..3072
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3152..3740
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3205..3225)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3623..3634)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4045..4225
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5753..5772)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5986..6005)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6105..6127
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6165..6183)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6251..6355
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6356..6424,6425..7216)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6574..6593)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7387..7975
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7876..7895
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8129..8146
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8216..8236)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8221..8550
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8401..8536
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9684..9749
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9879..9899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10324..10458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10361..10380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10415..10434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10626..11259
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11306..11431
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11924..12157
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12342..12386
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12679..12796
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(12859..13160,1..198)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(12894..12917)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
       61 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
      121 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
      181 tcatctccgg gcctttcgac cggatcgaaa cccgggaaac tcgagcgcca ccatggcccg
      241 gtgctccaac tctatggcgc tgctgttcag ctttggcctc ctttggctgt gttcaggggt
      301 tttgggtact gacacagagg agcggctggt ggagcatctc ttggatcctt cccgctataa
      361 caagctgatc cgtccagcta ctaatggctc tgagctggtg actgtacagc tcatggtatc
      421 attggcacag ctcatcagtg tgcacgagcg ggagcagatc atgaccacca acgtctggct
      481 gacccagtgc tgggaagatt atcgcctcac atggaagcct gaggatttcg acaatatgaa
      541 gaaagtccga cttccttcta agcacatctg gctcccagat gtggttctat acaacaatgc
      601 tgacggcatg tacgaagtct ccttctattc caatgctgtg gtctcctatg atggcagcat
      661 cttttggcta ccgcctgcca tctacaagag cgcatgcaag attgaggtga agcacttccc
      721 atttgaccag cagaactgca ccatgaagtt ccgctcctgg acctacgacc gcactgagat
      781 tgacctggtg ctcaaaagcg atgtggccag cctggacgac ttcacaccta gtggggagtg
      841 ggacatcatc gcactgccag gccgacgcaa cgagaaccca gacgactcca cctacgtgga
      901 catcacctac gacttcatca ttcgtcgcaa accgctcttc tacaccatca acctcatcat
      961 cccctgcgta ctcatcacct cgctggccat cctggtcttc tacctgccct cagactgtgg
     1021 tgaaaagatg acactttgta tttctgtgct gctggcgctc acggtgttcc tgctgctcat
     1081 ctccaagatt gtgcctccca cctccctcga cgtaccgctg gtgggaaagt acctcatgtt
     1141 caccatggtg ctagtcacct tctccatcgt cactagcgtg tgtgtgctca atgtgcacca
     1201 ccgttcgcct accacgcaca ccatggcgcc ctgggtcaag gtggtcttcc tggagaagct
     1261 gcccaccctc ctcttcctgc agcagccacg ccaccgctgt gcacgtcaac gcctgcgctt
     1321 gcgaaggcgc cagcgggaac gtgagggggc aggcacacta ttcttccgcg aaggtcctgc
     1381 agctgaccca tgtacctgct ttgtcaatcc tgcatcaatg cagggcttgg ctggggcttt
     1441 ccaggctgag cccgctgcag ccggtctggg gcgctcgatg ggcccatgca gctgtggcct
     1501 ccgggaagct gtggacggtg tacgcttcat tgcggaccat atgcgaagtg aagatgatga
     1561 ccagagtgtg agggaggatt ggaaatacgt tgccatggtg atcgaccgcc tgttcctctg
     1621 gatctttgtc tttgtctgtg tctttgggac cattggcatg ttcctgcagc ctctcttcca
     1681 gaactacact gccacgacct tccttcactc tgaccactca gctcccagct ccaagtgaat
     1741 cccgcggcca tggcggccgg gagcatgcga cgtcgggccc ggtaccgaat tcatagctag
     1801 catcgatccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     1861 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     1921 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     1981 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     2041 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     2101 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     2161 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     2221 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     2281 ggcctcggta cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     2341 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     2401 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2461 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2521 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2581 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     2641 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2701 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2761 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2821 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     2881 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2941 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     3001 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     3061 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     3121 ggccgcgact gtcgactgca gaattcccga taatcaacct ctggattaca aaatttgtga
     3181 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3241 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3301 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3361 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3421 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     3481 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     3541 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     3601 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     3661 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     3721 cctttgggcc gcctccccgc atcgggaatt cgaagcttga gctcgatcga cgcgtcgtcg
     3781 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     3841 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     3901 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     3961 gggactggaa gggctaattc actcccaacg aagacaaaat cgtcgagaga tgctgcatat
     4021 aagcagctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     4081 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4141 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4201 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     4261 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattataa tagatttagc
     4321 aggaattgaa ctaggagtgg agcacacagg caaagctgca gaagtacttg gaagaagcca
     4381 ccagagatac tcacgattct gcacatacct ggctaatccc agatcctaag gattacatta
     4441 agtttactaa catttatata atgatttata gtttaaagta taaacttatc taatttacta
     4501 ttctgacaga tattaattaa tcctcaaata tcataagaga tgattactat tatccccatt
     4561 taacacaaga ggaaactgag agggaaagat gttgaagtaa ttttcccaca attacagcat
     4621 ccgttagtta cgactctatg atcttctgac acaaattcca tttactcctc accctatgac
     4681 tcagtcgaat atatcaaagt tatggacatt atgctaagta acaaattacc cttttatata
     4741 gtaaatactg agtagattga gagaagaaat tgtttgcaaa cctgaatagc ttcaagaaga
     4801 agagaagtga ggataagaat aacagttgtc atttaacaag ttttaacaag taacttggtt
     4861 agaaagggat tcaaatgcat aaagcaaggg ataaattttt ctggcaacaa gactatacaa
     4921 tataacctta aatatgactt caaataattg ttggaacttg ataaaactaa ttaaatatta
     4981 ttgaagatta tcaatattat aaatgtaatt tacttttaaa aagggaacat agaaatgtgt
     5041 atcattagag tagaaaacaa tccttattat cacaatttgt caaaacaagt ttgttattaa
     5101 cacaagtaga atactgcatt caattaagtt gactgcagat tttgtgtttt gttaaaatta
     5161 gaaagagata acaacaattt gaattattga aagtaacatg taaatagttc tacatacgtt
     5221 cttttgacat cttgttcaat cattgatcga agttctttat cttggaagaa tttgttccaa
     5281 agactctgaa ataaggaaaa caatctatta tatagtctca cacctttgtt ttacttttag
     5341 tgatttcaat ttaataatgt aaatggttaa aatttattct tctctgagat catttcacat
     5401 tgcagataga aaacctgaga ctggggtaat ttttattaaa atctaattta atctcagaaa
     5461 cacatcttta ttctaacatc aatttttcca gtttgatatt atcatataaa gtcagccttc
     5521 ctcatctgca ggttccacaa caaaaatcca accaactgtg gatcaaaaat attgggaaaa
     5581 aattaaaaat agcaatacaa caataaaaaa atacaaatca gaaaaacagc acagtataac
     5641 aactttattt agcatttaca atctattagg tattataagt aatctagcca gatcctctac
     5701 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     5761 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     5821 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     5881 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     5941 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga
     6001 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     6061 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     6121 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     6181 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     6241 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6301 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6361 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6421 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6481 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6541 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6601 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6661 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6721 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6781 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6841 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6901 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6961 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7021 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7081 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7141 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7201 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7261 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7321 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7381 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7441 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7501 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7561 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7621 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7681 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7741 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7801 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7861 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7921 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7981 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8041 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8101 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8161 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat
     8221 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8281 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8341 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8401 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8461 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8521 ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat
     8581 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
     8641 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
     8701 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
     8761 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
     8821 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg
     8881 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
     8941 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
     9001 atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat
     9061 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac
     9121 tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta
     9181 ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt
     9241 ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat
     9301 gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc
     9361 gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg
     9421 taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat
     9481 tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac
     9541 cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg
     9601 ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     9661 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     9721 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     9781 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     9841 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     9901 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     9961 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
    10021 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
    10081 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
    10141 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
    10201 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
    10261 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
    10321 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    10381 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    10441 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
    10501 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
    10561 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    10621 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
    10681 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
    10741 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
    10801 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
    10861 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
    10921 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
    10981 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
    11041 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
    11101 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    11161 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    11221 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
    11281 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
    11341 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
    11401 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
    11461 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
    11521 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
    11581 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
    11641 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
    11701 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
    11761 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
    11821 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    11881 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    11941 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
    12001 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
    12061 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
    12121 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    12181 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    12241 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
    12301 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    12361 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
    12421 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
    12481 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
    12541 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
    12601 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccga attcacaaat
    12661 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    12721 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    12781 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggctgatacg
    12841 cggatcgaga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
    12901 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
    12961 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
    13021 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
    13081 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
    13141 ggaagcgggt aggcctttgg
//
