LOCUS       sequence-32922-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   French ME, Kretzmann BR, Hicke L
  TITLE     Regulation of the RSP5 ubiquitin ligase by an intrinsic 
            ubiquitin-binding site.
  JOURNAL   J Biol Chem. 2009 May 1. 284(18):12071-9.
  PUBMED    19252184
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 May 1. 284(18):12071-9."
COMMENT     Sequence Label: pET-30 Ubwt (2)
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             103..147
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             178..189
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             190..417
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             190..417
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             499..516
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(569..587)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      583..630
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggaattccc tctgaatatt ttgtttactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtct ggtgccacgc ggttctggta tgaaagaaac cgctgctgct
      121 aaattcgaac gccagcacat ggacagccca gatctgggta ccggtggtgg ctccggtatt
      181 gagggtcgca tgcagatctt cgtcaagacg ttaaccggta aaaccataac tctagaagtt
      241 gaatcttccg ataccatcga caacgttaag tcgaaaattc aagacaagga aggcattcca
      301 cctgatcaac aaagattgat ctttgccggt aagcagctcg aggacggtag aacgctgtct
      361 gattacaaca ttcagaagga gtcgacctta catcttgtct taagactaag aggtggttga
      421 ggctctaact ctcctctggc catggcgata tcggatccga attcgagctc cgtcgacaag
      481 cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
      541 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      601 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
      661 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      721 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc cccttccttt
      781 ctcgccacgt tcgccgg
//
