LOCUS       sequence-329221-        8002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Cre-dependent Cas9 under control of the TRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Sabatini PV, Wang J, Rupp AC, Affinati AH, Flak JN, Li C, Olson DP, 
            Myers MG Jr
  TITLE     tTARGIT AAVs mediate the sensitive and flexible manipulation of 
            intersectional neuronal populations in mice.
  JOURNAL   eLife. 2021 Mar 11;10. pii: 66835. doi: 10.7554/eLife.66835.
  PUBMED    33704065
REFERENCE   2  (bases 1 to 8002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-03-11"; volume: "10. pii: 66835. doi"; pages: " 
            10.7554/eLife.66835"
COMMENT     Sequence Label: pAAV-TRE-DIO-Cas9
FEATURES             Location/Qualifiers
     source          1..8002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     protein_bind    join(287..305,306..328,329..347,348..370,371..389,390..412,
                     413..431,432..454,455..473,474..496,497..515,516..538,
                     539..557)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        590..628
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    complement(763..796)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(807..840)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(909..989)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(996..1043)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(1044..4199)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Staphylococcus aureus 
                     Type II CRISPR/Cas system"
                     /label=SaCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG
                     RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF
                     SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV
                     RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW
                     KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ
                     IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI
                     IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI
                     NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK
                     VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK
                     YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ
                     EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS
                     VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN
                     KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF
                     ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN
                     DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD
                     NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL
                     DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN
                     RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
                     IKKG"
     CDS             complement(4224..4244)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4247..4256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4305..4338
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4349..4382
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    4406..4994
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4459..4479)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4877..4888)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5029..5046)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5035..5242
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5265..5405
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5265..5394
                     /label=AAV2 ITR
     rep_origin      5480..5935
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5567..5586)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5777..5798
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5952..5971)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6071..6093
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6131..6149)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6217..6321
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6322..6390,6391..7182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6540..6559)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7353..7941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7842..7861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgaaacaat tctgcaggaa ttcggggccg cggaggctgg
      181 atcggtcccg gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct
      241 gacggttcac taaacgagct ctgcttatat aggtcgagtt taccactccc tatcagtgat
      301 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      361 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      421 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
      481 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
      541 cctatcagtg atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt
      601 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      661 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc
      721 cgaattcgag ctcggtaccc ggggatcctc tagtcgacgt cgataacttc gtatagcata
      781 cattatacga agttatccga atcgcaataa cttcgtatag gatactttat acgaagttat
      841 ttggcgcgcc aaatttaaat aacgcaacgt accttcgagg gggccccagc gagctctagg
      901 aattcttaag cgtaatctgg aacatcgtat gggtaagcgt aatctggaac atcgtatggg
      961 taagcgtaat ctggaacatc gtatgggtag gatccctttt tcttttttgc ctggccggcc
     1021 tttttcgtgg ccgccggcct tttgcccttt ttgatgatct gagggtgctt cttagatttc
     1081 acttcataca ggttgcccag aatgtctgtg ctgtacttct taatgctctg ggtcttggag
     1141 gcgattgtct taatgatcct ggggggcctc ttgtcgttca tgttttccag gtactcgcgg
     1201 taggtgatgt cgatcatgtt cacttcgatc cggttcagca ggtcgttgtt cacgccgatc
     1261 actctataca gctcgccgtt gatcttgatc agatcgttgt tgtagaagga ggcgataaac
     1321 tcggcctggt tgctgatctt cttcagcttc ttagcttcct catagcactt gctattcact
     1381 tcgtagtagt tttctttttt gatcacatcc agattcttca cggtcacgaa cttgtacacg
     1441 ccattgtcca ggtacacgtc gaatctgtag ggcttcaggg acagcttcac gaccttgttt
     1501 ctgctgttgg ggtagtcgtc ggtgatgtcc agatgggcgt tcagtttgtt gccgtaatac
     1561 ttaatcttct tgatcacggg gccgttgtcc tttttggagt acttggtcag gtagttcccg
     1621 gtttcctcgt agtacttgta caggggattc ttctcgtcgc cgtactgttc cataatcagc
     1681 ttcagtttct ggtaggtctg ggggtcgtgg tggtacatca gcagcttttc ggggctcttg
     1741 ttgatcagct ttttcagctt gtcattgtcc ttgtcgtaca ggccgttcag attgttcacg
     1801 atcagggtgt tgcccttgtc gtccttccgg gtggagtaca gggtgtcgtt aatcagctct
     1861 ctattaggct tcttgtccac ccggtggctg tacttgtagt ccttgaagtc cttaatgtgc
     1921 ttgatctggt ggggggtgat gaagatctct ttgtactcct gctcggtttc gatctcgggc
     1981 atgctctcgg cctgcttttc ctcgaacatc tggttttcca tcactttttt ggccttgtcc
     2041 agtttcttcc actctttgaa gatgaaatcg gcgttggcaa tgatcagggc gtcctcggcg
     2101 tggtgcttgt accccttgtt ccgctctttc ttaaacttcc acttccgccg cagaaagctg
     2161 gtgaagccgc cattgatgga cttcactttc acgtccaggt tgttcactct gaagtagctc
     2221 cgcagcaggt tcatcaggcc tctggtggcg tatctggtat ccaccaggtt ccggttgatg
     2281 aagtctttct gcacggagaa cctgttgatg tcccgttctt ccagcagata ctctttcttg
     2341 gtcttgctga ttctgccctt gcccttggcc agattcagga tgtgcttctt gaaggtttcg
     2401 tagctgatct tgctgtcgct gctgctcagg tactggaatg gggtccggtt gcccttcttg
     2461 ctgttttctt cctgcttcac gagcaccttg ttgttgaagc tgttgtcgaa ggacacgctt
     2521 ctggggatga tgtggtccac ctcatagttg aaggggttgt tcagcagatc ttccagaggg
     2581 atggcttcca ggctgtacag gcacttgcct tcctgcatgt cgtgcagctt gatcttctcg
     2641 atcaggtact tggcgttctc tttgccggtg gtccggatga tttcctcgat ccgctcgttg
     2701 gtctgccggt tccgcttctg catctcgttg atcattttct gggcgtcctt ggagttcttc
     2761 tcgcgggcca gctcgataat gatgtcgttg ggcaggccgt acttcttgat gatggcgttg
     2821 atcactttga tgctctggat gaagcttctc ttcacgacgg ggctcaggat gaagtcgtcc
     2881 accagggtgg tggggatctc tttctgctgg gacaggtcca ccttcttggg caccagcttc
     2941 agccggttga agatagcgat ctggttgtcg ttggtgtgcc acagctcgtc caggatcagg
     3001 ttgatggcct tcaggctcag gttgtgggtg ccggtatagc ccttcagatt agagatctgc
     3061 tcgatctctt cctgggtcag ctcggagttc agattggtca gttcttcctg gatgtcctcg
     3121 ctgctctggt agatggtcag gatcttggca atctgatcca gcagctcggc gttctcaata
     3181 atctctttcc gggcggtaat gtccttgatg tcgtggtaca ccttcaggtt ggtgaactcg
     3241 ggcttgccgg tgctggtcac tctgtagccc ttaatatcct cttcgttcac gaggatttct
     3301 ttggcgatct gcttcagggt gggcttcttc ttctgcttga acacgttctc gatgatctgg
     3361 aacttctcgt aatattccag cttctcgttc tcgtccctgg tgatcacgag attgttcagg
     3421 tcgttcaggg cgttgtacag gtcggcgttg taggcgtact tcacgctccg cagttcctcg
     3481 gggaagtagg tgcagtggcc catcagcatc tcgtaccatt ctttgatgtc cttccagccg
     3541 aaggggctgc cctcgccagg tccctcatag taggtccgcc gggtttccag caggtcgatg
     3601 taggtgtcga tgaagctctg gtccagctgg tggtaggcct tctgcacctt cagcagctgt
     3661 ttggcttctt tcacgtagtc gctggtcttg aatctgttga tgctgccccg cacttcgccg
     3721 tctttcttca gccgttccag ctgcagttcg gccacgtatt tctcttccag ggccttgctg
     3781 ttccggctga tctgctcttt ggtggacagc tcgttgccgg tgtcctcttc cacctcgttc
     3841 acgttgtgca cgcctcttct cttggccagg tgcagcaggg cggcagagaa ctcttcctcg
     3901 ctcagcttct ggctcaggcc cttcactctg gcctcgtagg ggttgatgcc gctcagctcg
     3961 ctgtggtcgg tcagcaggtt gtagtcgaac agcagcttct tcactctctg gattctatgc
     4021 cgcctccgcc gcttcagcct tctggcgcct ctcttgctcc gcctgccctc gttgttttcc
     4081 acgttggcct ctttgaacag ccgcacgccg gcatcgatca cgtcccgtgt ctcgtagtcg
     4141 atgatgccgt agcccacgct ggtgatgccg atgtccaggc ccaggatgta gttccgcttg
     4201 gctgctggga ctccgtggat accgaccttc cgcttcttct ttggggccat ggtggcaccg
     4261 gtagttagcg ctagctagtt ggcgcgccaa cctggccggc cttgataact tcgtataatg
     4321 tatgctatac gaagttatcc gaatcgcaat aacttcgtat aaagtatcct atacgaagtt
     4381 atcgaagatc atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     4441 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4501 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4561 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4621 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4681 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4741 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4801 atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc
     4861 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4921 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4981 ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tcgatctgcc tcgactgtgc
     5041 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5101 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5161 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5221 acaatagcag gcatgctggg gacacgtgcg gaccgagcgg ccgcaggaac ccctagtgat
     5281 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     5341 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     5401 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     5461 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5521 gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc
     5581 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5641 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     5701 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5761 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg
     5821 ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag
     5881 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     5941 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     6001 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     6061 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     6121 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6181 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6241 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6301 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6361 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6421 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6481 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6541 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6601 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6661 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6721 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6781 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6841 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6901 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6961 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7021 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7081 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7141 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7201 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7261 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7321 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7381 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7441 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7501 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7561 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7621 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7681 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7741 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7801 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7861 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7921 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7981 tgctggcctt ttgctcacat gt
//
