LOCUS       sequence-329226-        5960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5960)
  TITLE     SGC Toronto Q3-Q4 2020
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CORO6
FEATURES             Location/Qualifiers
     source          1..5960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             181..201
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    1566..1700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1603..1622)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1657..1676
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1729..1894
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2078..2532
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2165..2184)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2374..2395
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2558..2662
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2663..2731,2732..3523)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2881..2900)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3694..4282
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4183..4202
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4436..4453
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4587..4811
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4878..5411)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5581..5586,5587..5603,5604..5609))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg catcatcatc atcatcacag cagcggcaga
      181 gaaaacttgt atttccaggg catgtcgaga cgtgtggttc gtcagagtaa attccgtcac
      241 gttttcggcc aagctgcgaa agcagaccaa gcctatgaag acattcgcgt gtctaaagtc
      301 acatgggaca gtagcttctg cgcggtgaac cctaagttcc tggcaattat agtagaggct
      361 ggtggaggtg gtgcctttat cgtcctcccg ttggccaaaa caggcagggt cgataaaaac
      421 tatcctttgg taacgggtca cacggcaccg gtcctcgaca ttgactggtg tcctcataat
      481 gataatgtta ttgcgtcagc ctctgatgat actactatca tggtttggca gatccccgac
      541 tacaccccga tgcgcaacat cactgagccc ataatcacgc ttgagggcca cagcaaacgt
      601 gtaggtatct tgtcatggca tcccacggct agaaatgttc tcctcagtgc tggtggtgat
      661 aatgtgataa tcatctggaa cgtcggaacg ggcgaggtgc tgctgagcct ggacgatatg
      721 cacccagacg tgatacactc agtttgctgg aattctaacg gctcacttct ggcgacaaca
      781 tgtaaagata aaacactcag aattatcgac ccacgtaagg gacaggtggt tgcggagcaa
      841 gccaggccgc atgagggtgc gaggccactc cgtgctgtct tcaccgcaga cggcaaactc
      901 ttgtccactg gtttttcacg tatgtcggaa cgccagttgg cgctgtggga tccgaataac
      961 ttcgaagaac cggtggcctt gcaggaaatg gatacgagca atggtgtcct cttgccgttt
     1021 tacgatccgg acagctcgat agtttacctg tgcggtaagg gtgatagtag cattcgttac
     1081 tttgagatta ctgatgagcc tccatttgtt cattatctga atactttttc gagtaaagag
     1141 ccacagaggg gcatgggatt tatgccgaaa cgtggtttgg atgtatcgaa gtgtgaaata
     1201 gcgcgttttt acaagctgca cgagaggaag tgtgaaccga taattatgac tgtaccaagg
     1261 aagtccgatc tgtttcaaga cgacctttac ccagacaccc cgggcccaga gcccgctttg
     1321 gaagcagacg aatggttgag cggacaggac gcagagccgg ttttgatatc tcttagggat
     1381 ggatatgttc ccccaaagca ccgtgagctt agagtaacga agaggaattg atgacgaagc
     1441 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
     1501 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     1561 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1621 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1681 tatcttatca tgtctggatc tgatcactga tatcgcctag gagatccgaa ccagataagt
     1741 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
     1801 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
     1861 ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt
     1921 ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct
     1981 ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga
     2041 atatcaacgc ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     2101 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2161 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2221 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2281 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2341 cgccctttga cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa
     2401 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2461 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2521 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2581 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2641 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2701 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2761 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     2821 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2881 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     2941 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3001 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3061 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3121 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3181 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3241 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3301 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3361 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3421 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3481 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3541 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3601 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3661 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3721 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3781 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3841 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3901 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3961 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4021 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4081 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4141 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4201 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4261 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4321 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4381 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4441 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     4501 cggtatttca caccgcatag accagccgcg taacctggca aaatcggtta cggttgagta
     4561 ataaatggat gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa
     4621 atagatctaa actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc
     4681 agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc
     4741 attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatggtaa
     4801 agactatatt cgcggcgttg tgacaattta ccgaacaact ccgcggccgg gaagccgatc
     4861 tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg catcacttct
     4921 tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta atctgcttgc
     4981 acgtagatca cataagcacc aagcgcgttg gcctcatgct tgagcagatt gatgagcgcg
     5041 gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatagat atagatctca
     5101 ctacgcggct gctcaaactt gggcagaacg taagccgcga gagcgccaac aaccgcttct
     5161 tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga gcaagttccc gaggtaatcg
     5221 gagtccggct gatgttggga gtaggtggct acgtctccga actcacgacc gaaaagatca
     5281 agagcagccc gcatggattt gacttggtca gggccgagcc tacatgtgcg aatgatgccc
     5341 atacttgagc cacctaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     5401 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     5461 atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     5521 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     5581 agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt
     5641 gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg
     5701 aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc
     5761 tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg
     5821 tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg
     5881 gaaggcgagc atcgtttgtt cgcccaggac tctagctata gttctagtgg ttggctacgt
     5941 atactccgga atattaatag
//
