LOCUS       sequence-329227-        5684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5684)
  TITLE     SGC Toronto Q3-Q4 2020
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KIF21A
FEATURES             Location/Qualifiers
     source          1..5684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             181..201
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    1290..1424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1327..1346)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1381..1400
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1453..1618
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1802..2256
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1889..1908)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2098..2119
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2282..2386
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2387..2455,2456..3247)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2605..2624)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3418..4006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3907..3926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4160..4177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4311..4535
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4602..5135)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5305..5310,5311..5327,5328..5333))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg catcatcatc atcatcacag cagcggcaga
      181 gaaaacttgt atttccaggg ccttcagtgt attcacatag ctgaagggca tacaaaagct
      241 gtgctctgtg tggattctac tgatgatctc ctcttcactg gatcaaaaga tcgtacttgt
      301 aaagtatgga atctggtgac tgggcaggaa ataatgtcac tggggggtca tcccaacaat
      361 gtcgtgtctg taaaatactg taattatacc agtttggtct tcactgtatc aacatcttat
      421 attaaggtgt gggatatcag agattcagca aagtgcattc gaacactaac gtcttcaggt
      481 caagttactc ttggagatgc ttgttctgca agtaccagtc gaacagtagc tattccttct
      541 ggagagaacc agatcaatca aattgcccta aacccaactg gcaccttcct ctatgctgct
      601 tctggaaatg ctgtcaggat gtgggatctt aaaaggtttc agtctacagg aaagttaaca
      661 ggacacctag gccctgttat gtgccttact gtggatcaga tttccagtgg acaagatcta
      721 atcatcactg gctccaagga tcattacatc aaaatgtttg atgttacaga aggagctctt
      781 gggactgtga gtcccaccca caattttgaa ccccctcatt atgatggcat agaagcacta
      841 accattcaag gggataacct atttagtggg tctagagata atggaatcaa gaaatgggac
      901 ttaactcaaa aagaccttct tcagcaagtt ccaaatgcac ataaggattg ggtctgtgcc
      961 ctgggagtgg tgccagacca cccagttttg ctcagtggct gcagaggggg cattttgaaa
     1021 gtctggaaca tggatacttt tatgccagtg ggagagatga agggtcatga tagtcctatc
     1081 aatgccatat gtgttaattc cacccacatt tttactgcag ctgatgatcg aactgtgaga
     1141 atttggaagg cttgatgacg aagcttgtcg agaagtacta gaggatcata atcagccata
     1201 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     1261 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     1321 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     1381 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca ctgatatcgc
     1441 ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt
     1501 ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat
     1561 aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc
     1621 ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa
     1681 ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt catctcttcg
     1741 ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa tggcgaatgg
     1801 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     1861 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     1921 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     1981 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2041 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg
     2101 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     2161 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     2221 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     2281 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2341 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2401 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2461 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     2521 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2581 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     2641 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2701 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2761 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2821 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2881 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2941 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3001 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3061 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3121 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3181 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3241 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3301 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3361 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4081 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4141 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     4201 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct
     4261 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     4321 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     4381 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     4441 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     4501 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     4561 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     4621 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     4681 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     4741 tgcttgagca gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     4801 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     4861 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     4921 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     4981 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     5041 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     5101 ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
     5161 gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg
     5221 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     5281 gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt
     5341 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc
     5401 gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag
     5461 gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt
     5521 caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa
     5581 gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca ggactctagc
     5641 tatagttcta gtggttggct acgtatactc cggaatatta atag
//
