LOCUS       sequence-329228-        5675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5675)
  TITLE     SGC Toronto Q3-Q4 2020
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: WDR55
FEATURES             Location/Qualifiers
     source          1..5675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             181..201
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    1281..1415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1318..1337)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1372..1391
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1444..1609
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1793..2247
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1880..1899)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2089..2110
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2273..2377
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2378..2446,2447..3238)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2596..2615)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3409..3997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3898..3917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4151..4168
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4302..4526
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4593..5126)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5296..5301,5302..5318,5319..5324))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg catcatcatc atcatcacag cagcggcaga
      181 gaaaacttgt atttccaggg ctccatggaa gccccaaccc ggatccggga cactccggaa
      241 gacatcgtgc tggaagctcc ggctagtggg ctggcgttcc atccggcccg tgacctactg
      301 gctgcagggg acgtggacgg ggacgtgttc gtcttttcct actcttgcca agagggagaa
      361 accaaggagc tctggtcatc aggtcaccat ctcaaggcct gccgagctgt ggccttctct
      421 gaagatgggc agaagctcat tactgtctcc aaggacaaag ccatccatgt tctagatgtg
      481 gagcagggcc aactggaaag acgtgtttcc aaggctcatg gtgcccccat caacagtctt
      541 ctgctggtgg atgagaatgt tctggccact ggggatgaca caggtggtat ccgtctctgg
      601 gaccagcgga aggagggccc cttaatggat atgaggcaac atgaagagta catcgcagac
      661 atggctctgg atccagccaa aaagctgctg ctgacagcca gcggggatgg ctgccttggc
      721 atcttcaaca ttaagaggcg tcggtttgag ctgctctcag aacctcagtc tggggacctg
      781 acctctgtca ctctcatgaa atgggggaag aaggtagcct gtggctccag tgaaggtacc
      841 atctacctct tcaattggaa tggctttggg gccacaagtg accgctttgc cctgagagct
      901 gaatctatcg actgcatggt tccagtcacc gagagtctgc tgtgtactgg ctccactgat
      961 ggagtcatca gggctgtgaa catcctaccg aaccgagtgg tgggcagtgt gggccagcac
     1021 actggggagc ctgtggagga gctggccctc tcccactgtg gccgcttcct ggccagtagt
     1081 ggccatgacc agcgcctcaa gttttgggac atggcccagc tgcgagctgt ggtggtggat
     1141 gactgatgac gaagcttgtc gagaagtact agaggatcat aatcagccat accacatttg
     1201 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     1261 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     1321 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1381 ccaaactcat caatgtatct tatcatgtct ggatctgatc actgatatcg cctaggagat
     1441 ccgaaccaga taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta
     1501 gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa
     1561 aaactccatt tccacccctc ccagttccca actattttgt ccgcccacag cggggcattt
     1621 ttcttcctgt tatgttttta atcaaacatc ctgccaactc catgtgacaa accgtcatct
     1681 tcggctactt tttctctgtc acagaatgaa aatttttctg tcatctcttc gttattaatg
     1741 tttgtaattg actgaatatc aacgcttatt tgcagcctga atggcgaatg ggacgcgccc
     1801 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     1861 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     1921 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     1981 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     2041 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tcttaatagt ggactcttgt
     2101 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     2161 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     2221 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     2281 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2341 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     2401 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     2461 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     2521 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     2581 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     2641 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     2701 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     2761 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     2821 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2881 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     2941 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     3001 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     3061 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     3121 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     3181 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     3241 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     3301 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     3361 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3421 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3481 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3541 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3601 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3661 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3721 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3781 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3841 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3901 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3961 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4021 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4081 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     4141 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     4201 ccttacgcat ctgtgcggta tttcacaccg catagaccag ccgcgtaacc tggcaaaatc
     4261 ggttacggtt gagtaataaa tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt
     4321 cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag
     4381 ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc
     4441 taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg
     4501 ccaagggcat ggtaaagact atattcgcgg cgttgtgaca atttaccgaa caactccgcg
     4561 gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca
     4621 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca
     4681 ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagc
     4741 agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca
     4801 tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg
     4861 ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag
     4921 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca
     4981 cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat
     5041 gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc
     5101 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     5161 ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc
     5221 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     5281 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc
     5341 atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt
     5401 ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg
     5461 gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc
     5521 ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc
     5581 atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct
     5641 agtggttggc tacgtatact ccggaatatt aatag
//
