LOCUS       sequence-329235-        9714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression and purification of USP47..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Parsons JL, Dianova II, Khoronenkova SV, Edelmann MJ, Kessler BM, 
            Dianov GL
  TITLE     USP47 is a deubiquitylating enzyme that regulates base excision 
            repair by controlling steady-state levels of DNA polymerase beta.
  JOURNAL   Mol Cell. 2011 Mar 4;41(5):609-15. doi: 
            10.1016/j.molcel.2011.02.016.
  PUBMED    21362556
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2011.02"; journalName: "Mol Cell"; date: 
            "2011-03-4- 4"; volume: "41"; issue: "5"; pages: "609-15"
COMMENT     Sequence Label: pDEST26-USP47
FEATURES             Location/Qualifiers
     source          1..9714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        197..500
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        501..704
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     655..675
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     701..725
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             826..843
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    853..877
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4769..4793)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4825..4844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4826..4844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4855..4872)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4855..4871)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4864..4886)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    5276..5410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5313..5332)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5367..5386
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5536..5991
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5623..5642)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5833..5854
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6108..6465
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6108..6299
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6131..6151)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6316..6451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6516..7310
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6570..6589)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7180..7199
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7374..7422
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7463..7482)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7582..7604
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        7728..7832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7833..7901,7902..8693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8051..8070)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8864..9452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9353..9372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9606..9623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(join(9697..9714,1..87))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
       61 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
      121 agaaaccatt attatcatga cattaaccta taaaaatagg cgtattacga ggccctttca
      181 cgcattagat gcatgtcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      241 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      301 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      361 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      421 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      481 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      541 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      601 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      661 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      721 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      781 ccagcctccg gactctagcc taggccgcgg accatggcgt actaccatca ccatcaccat
      841 cactctagat caacaagttt gtacaaaaaa gcagaagggc cgtcaaggcc caccatggtg
      901 cccggcgagg agaaccaact ggtcccgaaa gaggcaccac tggatcatac cagtgacaag
      961 tcacttctcg acgctaattt tgagccagga aagaagaact ttctgcattt gacagataaa
     1021 gatggtgaac aacctcaaat actgctggag gattccagtg ctggggaaga cagtgttcat
     1081 gacaggttta taggtccgct tccaagagaa ggttctgggg gttctaccag tgattatgtc
     1141 agccaaagct actcctactc atctattttg aataaatcag aaactggata tgtgggacta
     1201 gtaaaccaag caatgacttg ctatttgaat agccttttgc aaacactttt tatgactcct
     1261 gaatttagga atgcattata taagtgggaa tttgaagaat ctgaagaaga tccagtgaca
     1321 agtattccat accaacttca aaggcttttt gttttgttac aaaccagcaa aaagagagca
     1381 attgaaacca cagatgttac aaggagcttt ggatgggata gtagtgaggc ttggcagcag
     1441 catgatgtac aagaactatg cagagtcatg tttgatgctt tggaacagaa atggaagcaa
     1501 acagaacagg ctgatcttat aaatgagcta tatcaaggca agctgaagga ctacgtgaga
     1561 tgtctggaat gtggttatga gggctggcga atcgacacat atcttgatat tccattggtc
     1621 atccgacctt atgggtccag ccaagcattt gctagtgtgg aagaagcatt gcatgcattt
     1681 attcagccag agattctgga tggcccaaat cagtattttt gtgaacgttg taagaagaag
     1741 tgtgatgcac ggaagggcct tcggtttttg cattttcctt atctgctgac cttacagctg
     1801 aaaagattcg attttgatta tacaaccatg cataggatta aactgaatga tcgaatgaca
     1861 tttcccgagg aactagatat gagtactttt attgatgttg aagatgagaa atctcctcag
     1921 actgaaagtt gcactgacag tggagcagaa aatgaaggta gttgtcacag tgatcagatg
     1981 agcaacgatt tctccaatga tgatggtgtt gatgaaggaa tctgtcttga aaccaatagt
     2041 ggaactgaaa agatctcaaa atctggactt gaaaagaatt ccttgatcta tgaacttttc
     2101 tctgttatgg ttcattctgg gagcgctgct ggtggtcatt attatgcatg tataaagtca
     2161 ttcagtgatg agcagtggta cagcttcaat gatcaacatg tcagcaggat aacacaagag
     2221 gacattaaga aaacacatgg tggatcttca ggaagcagag gatattattc tagtgctttc
     2281 gcaagttcca caaatgcata tatgctgatc tatagactga aggatccagc cagaaatgca
     2341 aaatttctag aagtggatga atacccagaa catattaaaa acttggtgca gaaagagaga
     2401 gagttggaag aacaagaaaa gagacaacga gaaattgagc gcaatacatg caagataaaa
     2461 ttattctgtt tgcatcctac aaaacaagta atgatggaaa ataaattgga ggttcataag
     2521 gataagacat taaaggaagc agtagaaatg gcttataaga tgatggattt agaagaggta
     2581 atacccctgg attgctgtcg ccttgttaaa tatgatgagt ttcatgatta tctagaacgg
     2641 tcatatgaag gagaagaaga tacaccaatg gggcttctac taggtggcgt caagtcaaca
     2701 tatatgtttg atctgctgtt ggagacgaga aagcctgatc aggttttcca atcttataaa
     2761 cctggagaag tgatggtgaa agttcatgtt gttgatctaa aggcagaatc tgtagctgct
     2821 cctataactg ttcgtgctta cttaaatcag acagttacag aattcaaaca actgatttca
     2881 aaggccatcc atttacctgc tgaaacaatg agaatagtgc tggaacgctg ctacaatgat
     2941 ttgcgtcttc tcagtgtctc cagtaaaacc ctgaaagctg aaggattttt tagaagtaac
     3001 aaggtgtttg ttgaaagctc cgagactttg gattaccaga tggcctttgc agactctcat
     3061 ttatggaaac tcctggatcg gcatgcaaat acaatcagat tatttgtttt gctacctgaa
     3121 caatccccag tatcttattc caaaaggaca gcataccaga aagctggagg cgattctggt
     3181 aatgtggatg atgactgtga aagagtcaaa ggacctgtag gaagcctaaa gtctgtggaa
     3241 gctattctag aagaaagcac tgaaaaactc aaaagcttgt cactgcagca acagcaggat
     3301 ggagataatg gggacagcag caaaagtact gagacaagtg actttgaaaa catcgaatca
     3361 cctctcaatg agagggactc ttcagcatca gtggataata gagaacttga acagcatatt
     3421 cagacttctg atccagaaaa ttttcagtct gaagaacgat cagactcaga tgtgaataat
     3481 gacaggagta caagttcagt ggacagtgat attcttagct ccagtcatag cagtgatact
     3541 ttgtgcaatg cagacaatgc tcagatccct ttggctaatg gacttgactc tcacagtatc
     3601 acaagtagta gaagaacgaa agcaaatgaa gggaaaaaag aaacatggga tacagcagaa
     3661 gaagactctg gaactgatag tgaatatgat gagagtggca agagtagggg agaaatgcag
     3721 tacatgtatt tcaaagctga accttatgct gcagatgaag gttctgggga aggacataaa
     3781 tggttgatgg tgcatgttga taaaagaatt actctggcag ctttcaaaca acatttagag
     3841 ccctttgttg gagttttgtc ctctcacttc aaggtctttc gagtgtatgc cagcaatcaa
     3901 gagtttgaga gcgtccggct gaatgagaca ctttcatcat tttctgatga caataagatt
     3961 acaattagac tggggagagc acttaaaaaa ggagaataca gagttaaagt ataccagctt
     4021 ttggtcaatg aacaagagcc atgcaagttt ctgctagatg ctgtgtttgc taaaggaatg
     4081 actgtacggc aatcaaaaga ggaattaatt cctcagctca gggagcaatg tggtttagag
     4141 ctcagtattg acaggtttcg tctaaggaaa aaaacatgga agaatcctgg cactgtcttt
     4201 ttggattatc atatttatga agaagatatt aatatttcca gcaactggga ggttttcctt
     4261 gaagttcttg atggggtaga gaagatgaag tccatgtcac agcttgcagt tttgtcaaga
     4321 cggtggaagc cttcagagat gaagttggat cccttccagg aggttgtatt ggaaagcagt
     4381 agtgtggacg aattgcgaga gaagcttagt gaaatcagtg ggattccttt ggatgatatt
     4441 gaatttgcta agggtagagg aacatttccc tgtgatattt ctgtccttga tattcatcag
     4501 gatttagact ggaatcctaa agtttctacc ctgaatgtct ggcctcttta tatctgtgat
     4561 gatggtgcgg tcatatttta tagggataaa acagaagaat taatggaatt gacagatgag
     4621 caaagaaatg aactgatgaa aaaagaaagc agtcgactcc agaagactgg acatcgtgta
     4681 acatactcac ctcgtaaaga gaaagcacta aaaatatatc tggatggagc accaaataaa
     4741 gatctgactc aagactcagg cctcatgggc ccagctttct tgtacaaagt ggttgatcgc
     4801 gtgcatgcga cgtcatagct ctctccctat agtgagtcgt attataagct aggcactggc
     4861 cgtcgtttta caacgtcgtg actgggaaaa ctgctagctt gggatctttg tgaaggaacc
     4921 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     4981 aaatataaaa tttttaagtg tataatgtgt taaactagct gcatatgctt gctgcttgag
     5041 agttttgctt actgagtatg atttatgaaa atattataca caggagctag tgattctaat
     5101 tgtttgtgta ttttagattc acagtcccaa ggctcatttc aggcccctca gtcctcacag
     5161 tctgttcatg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     5221 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5281 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5341 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5401 tgtctggatc gatcctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5461 ttggctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     5521 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5581 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5641 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5701 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5761 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5821 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5881 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5941 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
     6001 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca
     6061 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
     6121 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     6181 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6241 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6301 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6361 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6421 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tgattcttct
     6481 gacacaacag tctcgaactt aaggctagag ccaccatgat tgaacaagat ggattgcacg
     6541 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     6601 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6661 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     6721 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     6781 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6841 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     6901 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     6961 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7021 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     7081 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     7141 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     7201 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     7261 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     7321 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgatggc cgcaataaaa
     7381 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gcgataagga
     7441 tccgcgtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     7501 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     7561 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     7621 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     7681 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7741 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7801 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7861 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7921 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7981 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8041 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8101 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8161 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8221 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8281 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8341 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8401 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8461 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8521 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8581 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8641 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8701 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8761 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9001 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9541 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9601 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9661 ccgcgcgttg gccgattcat taatgcagag cttgcaattc gcgctttttc aata
//
