LOCUS       sequence-32925-e         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Bryksin AV, Matsumura I
  TITLE     Rational design of a plasmid origin that replicates efficiently in 
            both gram-positive and gram-negative bacteria.
  JOURNAL   PLoS One. 2010  . 5(10):e13244.
  PUBMED    20949038
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(10):e13244."
COMMENT     Sequence Label: pBAV1K-T5-gfp
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..31
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    41..57
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(65..71,72..88,89..94,95..109))
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    73..89
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    114..135
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ctcctttacg catctagtat ttctcctctt tctctagtaa ttgttatccg ctcacaattg
       61 aatctattat aattgttatc cgctcacaaa gcaaataaat tttttatgat ttcctctaga
      121 agcggccgcg aattcgacgt caaattctat cataattgtg gtttcaaaat cggctccgtc
      181 gatactatgt tatacgccaa ctttgaaaac aactttgaaa aggctgtttt ctgtatttaa
      241 ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg
      301 gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc
      361 accggaattg aaaaaactga tcgaagaata ccgctgcgta aaagatacgg aaggaatgtc
      421 tcctgctaag gtatataagc tggtgggaga aaatgaaatc ctatatttaa aaatgacgga
      481 cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct
      541 ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa
      601 tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gggtatgaag atgaacaaag
      661 ccctgaaaag actatcgagc tgtatgcgga gtgcatca
//
