LOCUS       sequence-329262-       13283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  G418 resistant FC31 integrase mediated RMCE cassette for upgrading 
            MiMic insertions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13283)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 13283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31
FEATURES             Location/Qualifiers
     source          1..13283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    49..118
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        1546..1997
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     polyA_signal    5733..5867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5757..5776)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5811..5830
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    6060..6129
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             8410..10227
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     protein_bind    10183..10194
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     repeat_region   11059..11121
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      complement(11250..11838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11330..11349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11953..12055
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             12056..12715
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(12122..12141)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      12828..13256
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12915..12934)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13125..13146
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggatcc ggtctcagga ggaattggtc gacgatgtag gtcactgtct cgaagccgcg
       61 gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccacct cacccatctg
      121 gtccatcatg atgcgctgtc aggagctaga atcccaaaac aaactggtta ttgtggtagg
      181 tcatttgttt ggcagaaaga aaactcgaga aatttctctg gccgttattc gttattctct
      241 cttttctttt tgggtcgctc cctctctgca ctaatgctct ctcactctgt cacacagtaa
      301 acggcatact gctctcgttg gttcgagaga gcgcgcctcg aatgttcgcg aaaagagcgc
      361 cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt
      421 gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa
      481 tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc
      541 aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga gaacactcaa
      601 tactcgaatc catgtgggag tttattcttg acacagatat ttatgatata ataactgagt
      661 aagcttaaca taaggaggaa aacaaaatga ttgagcagga tggactgcac gctggctcgc
      721 cagctgcctg ggtggagcgt ctgttcggct acgattgggc ccagcagacc atcggctgct
      781 ccgatgctgc cgtgttccgt ctgtcggccc agggacgccc cgtgctgttc gtcaagaccg
      841 acctgagtgg agccctgaac gagctccagg atgaggcagc tcgtctgagc tggctggcca
      901 caacaggagt gccgtgtgct gccgtgctgg atgtggtgac cgaagctgga cgcgattggc
      961 tgctgctggg cgaggtgcca ggccaggacc tgctgtccag ccatctggcc ccagccgaga
     1021 aggtgtccat tatggccgat gctatgcgtc gcctgcacac cctggaccca gccacctgcc
     1081 cctttgacca ccaggccaag caccgtattg agcgtgcccg aacccgtatg gaggctggac
     1141 tggtggacca ggatgacctg gatgaggagc accagggcct ggctccagcc gagctgtttg
     1201 cccgtctgaa ggcccgtatg cccgatggcg aggacctggt ggtgacccac ggcgacgcct
     1261 gcctgcccaa cattatggtg gagaacggac gcttcagtgg cttcattgat tgtggacgcc
     1321 tgggcgtggc cgaccgttac caggatatcg ccctggccac ccgagatatc gccgaggagc
     1381 tgggaggcga gtgggccgac cgattcctgg tgctctacgg cattgcagcc ccagatagcc
     1441 agcgtattgc cttctaccgt ctgctggacg agttcttcta agcttggcaa taaaaagaca
     1501 gaataaaacg cacgggtgtt gggtcgtttg ttccgctgtc aggagctaga atcccaaaac
     1561 aaactggtta ttgtggtagg tcatttgttt ggcagaaaga aaactcgaga aatttctctg
     1621 gccgttattc gttattctct cttttctttt tgggtcgctc cctctctgca ctaatgctct
     1681 ctcactctgt cacacagtaa acggcatact gctctcgttg gttcgagaga gcgcgcctcg
     1741 aatgttcgcg aaaagagcgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat
     1801 tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg
     1861 cagctgaaca agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt
     1921 aaccagcaac caagtaaatc aactgcaact actgaaatct gccaagaagt aattattgaa
     1981 tacaagaaga gaactctcaa tactcgaatc catgtgggag tttattcttg acacagatat
     2041 ttatgatata ataactgagt aagcttaaca taaggaggaa aacaaaatgg tgaccggcgg
     2101 catggccagc aagtgggatc aaaagggcat ggatattgcc tatgaggagg ccgccttggg
     2161 ctacaaggag ggcggcgtcc ccattggcgg ctgcttgatc aacaacaagg atggcagcgt
     2221 gctgggacgc ggccacaaca tgcgcttcca gaagggcagc gccaccttgc acggcgagat
     2281 cagcaccctg gagaactgcg gacgcttgga gggcaaggtg tacaaggata ccaccctgta
     2341 caccaccctg agcccctgcg atatgtgcac cggcgccatc atcatgtacg gcatcccccg
     2401 ctgcgtggtg ggcgagaacg tgaacttcaa gagcaagggc gagaagtatc tgcaaacccg
     2461 cggacacgag gtggtggtgg tggatgatga gcgatgcaaa aagatcatga agcagttcat
     2521 cgatgagcgc ccccaggatt ggttcgagga tattggcgag gccagcgagc ccttcaagaa
     2581 cgtgtatctg ctgccccaga ccaaccaact gctgggcctg tacaccatca tccgcaacaa
     2641 gaacaccacc cgcccagatt tcatcttcta ttgtgaccgc atcatccgcc tgctggtcga
     2701 ggagggcctg aaccacctgc ccgtgcagaa gcagatcgtc gaaaccgata ccaacgagaa
     2761 cttcgagggc gttagcttca tgggcaagat ctgcggcgtg agcatcgtgc gtgccggcga
     2821 gagcatggag cagggcctgc gcgattgctg ccgcagcgtg cgcatcggca agatcctgat
     2881 ccagcgcgac gaggaaaccg ccctgcccaa gctgttttac gagaagctgc ccgaggatat
     2941 ttccgagcgc tacgtgttcc tgctcgatcc catgctggcc accggcggca gcgccatcat
     3001 ggccaccgag gtgctgatca agcgcggcgt gaagcccgag cgcatctact ttctgaacct
     3061 gatttgcagc aaggagggca ttgagaagta ccacgccgcc ttcccggagg tccgcatcgt
     3121 gaccggagcc ttggatcgcg gattggatga gaacaaatat ctggtgcccg gcctgggcga
     3181 tttcggcgat cgctactact gcgtgtaagc ttggcaataa aaagacagaa taaaacgcac
     3241 gggtgttggg tcgtttgttc cgctgtcagg agcgtacgac tagcacctga ctgtcgagcc
     3301 cagtggaaac ccttgaaatg cctttaagtc gagcccagtg gaaacccttg aaatgccttt
     3361 aagtcgagcc cagtggaaac ccttgaaatg cctttaagtc gagcccagtg gaaacccttg
     3421 aaatgccttt aagtcgagcc cagtggaaac ccttgaaatg cctttaagtc gagtagcagt
     3481 cgaggtaggt acgtcatgcc cctcccgagc tcgcccgggg atcgagcgca gcggtataaa
     3541 agggcgcggg gtggctgaga gcatcagttg tgaatgaatg ttcgagccga gcagacgtgc
     3601 cgctgccttc gttaatatcc tttgaataag ccaactttga atcacaagac gcataccaaa
     3661 ccaaatggga caggaggacc aggagctgct gatccgcgga ggctccaagc acccaagcgc
     3721 cgagcacctg aacaacggcg atagcggagc cgccagccag tcgtgcatca atcagggatt
     3781 tggccaggcc aagaattacg gaaccctgct gccccccagc cccccagagg atagcggaag
     3841 cggatccgga cagctggccg agaatctgac gtacgcctgg cataacatgg atatcttcgg
     3901 cgccgtgaac cagccaggca gtggctggcg tcagctggtg aaccgcaccc gcggactgtt
     3961 ctgcaatgag cgccatatcc cagccccacg caagcacctg ctgaagaatg tgtgcggagt
     4021 ggcctacccc ggcgagctgc tggccgtgat gggaagtagt ggcgccggaa agaccacgct
     4081 gctgaacgcc ctggccttcc gcagccccca gggcattcag gtgtccccaa gcggcatgcg
     4141 cctgctgaac ggccagcccg tggatgccaa ggagatgcag gcccgctgcg cctacgtgca
     4201 gcaggacgat ctgttcatcg gcagcctgac agcccgcgag catctgatct ttcaggccat
     4261 ggtccgcatg ccccgccacc tgacataccg tcagcgcgtg gcccgcgtgg accaggtcat
     4321 ccaggagctg agcctgagca agtgccagca caccattatt ggcgtgccag gccgcgtgaa
     4381 gggcctgagt ggcggagagc gcaagcgcct ggcctttgcc agcgaggccc tgaccgatcc
     4441 ccccctgctg atttgcgacg agccgaccag cggcctggat agcttcaccg cccactccgt
     4501 ggtgcaggtg ctgaagaagc tgagccagaa gggaaagacg gtcattctga cgatccacca
     4561 gcccagctcg gagctgttcg agctgtttga taagattctg ctgatggccg agggacgcgt
     4621 ggccttcctg ggaaccccaa gcgaggccgt ggatttcttc agctatgtgg gcgcccagtg
     4681 ccccacgaat tacaaccccg ccgacttcta tgtgcaagtg ctggccgtgg tgccaggacg
     4741 cgagattgag agccgcgatc gcattgccaa gatctgcgat aacttcgcca tctccaaggt
     4801 ggcccgcgac atggagcagc tgctggccac gaagaacctg gagaagcccc tggagcagcc
     4861 cgagaacggc tatacgtata aggctacctg gtttatgcag tttcgcgccg tgctgtggcg
     4921 cagctggctg agtgtgctga aggagcccct gctggtcaag gtccgcctga tccagaccac
     4981 gatggtggcc attctgatcg gcctgatctt cctgggacag cagctgacgc aggtcggagt
     5041 gatgaacatc aatggagcca tttttctgtt tctgacgaac atgacgttcc agaacgtgtt
     5101 cgccaccatc aacgtgttca ccagcgagct gcccgtgttc atgcgcgagg cccgcagccg
     5161 cctgtaccgc tgcgatacct acttcctggg aaagaccatt gccgagctgc cgctgttcct
     5221 gaccgtgccc ctggtgttta ccgccattgc ctaccccatg attggcctgc gtgccggagt
     5281 gctgcatttc tttaattgcc tggccctggt gaccctggtg gccaacgtgt cgaccagctt
     5341 cggctacctg atcagctgcg cctccagcag caccagcatg gccctgagcg tgggaccccc
     5401 cgtgattatc cccttcctgc tgttcggcgg attctttctg aactccggca gcgtgcccgt
     5461 gtacctgaag tggctgagct acctgagctg gtttcgctac gccaatgagg gcctgctgat
     5521 caatcagtgg gccgatgtgg agcccggcga gatctcctgc accagcagca acacgacctg
     5581 ccccagcagc ggaaaggtca tcctggagac actgaatttc agtgccgccg acctgccact
     5641 ggattatgtg ggcctggcca tcctgattgt gtccttccgc gtgctggcct atctggctct
     5701 gcgcctgcgc gctcgtcgca aggagtgagc ttgatccaga catgataaga tacattgatg
     5761 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     5821 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     5881 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttca gtatggcaag
     5941 cgctcgacga attaacaagc ttcgctctat ctcgctctct atctctctcg atcgctcgtg
     6001 ctaagcttaa gaccgtggag aacaaggaga accgctgtca ggagcatcat gatggaccag
     6061 atgggtgagg tggagtacgc gcccggggag cccaagggca cgccctggca cccgcaccgc
     6121 ggcttcgaga cagtgaccta catcgtcgac caattccgct gtcaggagct gcagctggtt
     6181 gtaggtgcag ttgcgcttct tgcacttggc gcacttgagg agatcggtct tggtgccctg
     6241 cacggtggcc agctgggcat cgttgatggc ctccttgacg aacttctcgc gcagcttctt
     6301 catctcatcg ctggccatct cctccggcgt cattttggcc agctgcttgg cagtgacggc
     6361 gccgcacata aagttgcctc gcaatccagg atttttgggg tccttcagat tggccacgcg
     6421 tgacctaatg cgattcttgt acttcatatc cgtgttattg aactcggagt aaatggcatc
     6481 ctccagttcg gcggccattt cctccggctc accgcatcct tcgggcactt caccaatctt
     6541 cagtgcggtg gccagcattt cgcggcattt gatgcggacc gcatctgtca tgccgccgga
     6601 gggaaacgag gtctgtgacg aggatgtcga gcctttcttc tctttatcct tggatgagct
     6661 ggatgaactg gacttatcct tgccggagat tgacgacgac gacttggcgg cactggtgga
     6721 cttcgaggca ctgctgttgt tggaggagcc ctccttagcg gagctgttgt tcggtgtggt
     6781 tggcgccggg ctggcgagga atcgtttcca gttcttgatc agcgtcttgg ccagagcgat
     6841 cacctcgtcg tccttgctac tcttgcgcag ctcgtttacg gtcatgccga tgcgcgtttt
     6901 ggtcagaatg tcgagattga tgttaagcgt ttgcagggcc ttcagcaggt ccagagcctg
     6961 atcctgtccc tgtacggtgg atatatgtat atttgtgaca tgatgcgcgg cacgttagct
     7021 aatcccgata cttactgtgc cgtcgctggc catcttgctc atcttctttt ggattcgaaa
     7081 cacttcctcc tccacgctca ttttggccca cttaatttga tcagaggtta ggttaactgg
     7141 cgtcggctgt cggctttaat cggtgctgcg ggtctgcgac agttgtttat gcggaagccg
     7201 ggctatttct ttttttccct ccaataaatg ttgtttacta tttttttcgt ttctgtattc
     7261 gcagaacgag ttgacagaac accctaacgc gttttagtgt taccgggcgg tggcttgtca
     7321 agaaatgcct tcccatggct aatacaattc ccaactggaa gtagtttggg tttcagaagt
     7381 caacagtgtt accgtatttt aatgtacatc tggcttttaa gcgagtacac acacatagca
     7441 ttgcgatgtt ttttaaactt gcactgcttg caagcagggc tccactgtat ttcaatgaaa
     7501 gaacagcgtg ttgggcgttt ttgaattata ctaattacct tcaataacaa ttccctatat
     7561 atcacttagt tttaataaat aaatcgtttg agtgtggtct agagatgatg tattatgatg
     7621 aagtgcggtt gttggggaca agcgggatac gtctgcgggt caaatcggcg gtgggacata
     7681 gtgagagggc aacggctcca aaaccaagcg ttgcgaacaa tcaccaacgc gccctggtac
     7741 gcgagaaatg ttgatatcgc acatgacctc agcatccacc ttcagtttga gaacaaatta
     7801 aatcaggcac ataccagaca accaggagaa cactgaggag gcgtcatcaa agatatcttt
     7861 ggacacgtgg cataaacaag ccaaactatt atctaatcaa tatttttata caaattttgt
     7921 atgtttccat gttatcttcg ttgctcctgt tagttactac gtttcatagc ccttaaattg
     7981 gttgcttaac gtaaaataaa attatattgt ataaaaataa agaggaattc acctataaca
     8041 aacgaaaaag taaataaata agaacttccc catgttaaaa atgccaccac catctttaca
     8101 tataagtatg tacatatgaa tgaatcactt aggttgcttg aatattacaa tttcattaat
     8161 agctaaatca catttgatgt gttagtggaa aacggctata tatataaatt atcgaaattg
     8221 tgaatatcga attgcgatag cacaatggga aattccacca ctagattttt ggtactttta
     8281 acagatcctt ttcggttttg cgttgcgcga agtgatctga acttatcaaa gtttgtaagg
     8341 taatacataa agtgaaaaag aattaatttg ctcttgaaag gcaggccaaa ttaaaaaaaa
     8401 aatatcaaaa tggacaccta tgccggtgcc tacgacagac agtcccggga gcgggaaaac
     8461 tccagtgcag ccagccccgc cacgcaacgg tcagctaacg aggataaggc ggccgacctg
     8521 cagagggagg tggagaggga cggcggcaga ttcagattcg taggccactt tagcgaggca
     8581 ccagggacta gtgcctttgg gacagcagag aggccagagt tcgagagaat cctgaatgag
     8641 tgccgagcgg gccgcctgaa catgatcata gtttacgatg tttcccggtt ctcacggctg
     8701 aaggttatgg acgccatccc tatcgtaagt gagctgctgg cactcggggt gactattgtt
     8761 agcactcagg agggagtgtt ccggcaggga aatgtcatgg acctgattca tttgattatg
     8821 cgcctggacg ccagccacaa agaaagcagc cttaagagcg cgaaaatctt ggacacaaag
     8881 aatcttcagc gagagcttgg cggctacgtg ggagggaagg ccccctacgg gttcgaactc
     8941 gttagcgaga caaaagaaat caccagaaac ggccgaatgg tcaacgtcgt cattaataaa
     9001 ctggcacatt ccacaacccc actgactggg ccatttgaat tcgaacccga cgtgatccgt
     9061 tggtggtggc gtgaaattaa gacgcacaag catctgccct ttaaacctgg atctcaggca
     9121 gccattcacc cgggctccat tactgggctt tgtaagcgga tggacgccga cgcagtccca
     9181 acgagggggg agacaatagg caaaaagact gcctcaagcg cgtgggaccc tgccacagtc
     9241 atgcgcattc tccgggatcc ccgcattgcc gggttcgccg cggaggtgat atataaaaag
     9301 aagcccgacg gtacacccac tacaaagatt gagggctacc ggatccagcg cgaccccatt
     9361 accctgcggc cagtcgaact ggattgcggg cctattatcg aaccggcaga atggtacgaa
     9421 cttcaggcct ggctggatgg acgggggaga ggcaaagggc tatctagagg ccaggctatc
     9481 ttgtccgcga tggacaaact ttattgcgaa tgcggcgccg tcatgacatc caagagaggc
     9541 gaagagagca tcaaagatag ttatagatgc agacgtcgca aagtggtcga tccctcagct
     9601 cccggacagc acgagggcac ctgtaatgtc agtatggccg cattagacaa gttcgtcgca
     9661 gaacgtatct ttaacaagat ccgtcacgct gaaggagatg aagaaaccct ggctctgtta
     9721 tgggaggccg ccagacgatt tgggaagctg acagaggctc cagaaaaatc tggcgaacgt
     9781 gccaacctgg tagctgagag agcggatgcg ctcaatgcac tggaggaact ctacgaggat
     9841 agggcagcgg gcgcctacga tgggccagtg ggtcgcaagc acttccggaa acagcaggca
     9901 gcactgacac ttagacaaca gggcgccgag gagaggctcg ccgagctaga ggccgctgag
     9961 gccccgaagc tccccctgga tcagtggttc cctgaggatg ccgacgctga tcccaccgga
    10021 cctaaaagct ggtgggggcg agcttccgtg gacgataaac gggttttcgt cggcctcttc
    10081 gtggacaaga tcgtggtgac taagagcacc actggcagag ggcaaggcac accaatcgag
    10141 aagcgcgcat ctatcacttg ggctaagcct cctaccgacg acgatgagga tgacgcacag
    10201 gacgggacag aggatgtagc agcctaagct taatgtatgg acatagattt caaataatta
    10261 aatgtaatgc agtaattgat gtaattagtt aaataagtta gatattaata acatattaat
    10321 tatatgtatt ataacgcata taataataaa atgcatattt aggatatgca agccattcga
    10381 attttctatt ttaatttcct tttacaaaga aatgtataac aaaatataat ttgaaaaaat
    10441 gttctggctc taattcgatt tcttttaagt attttgtgaa ctgcttttaa taacgagcgg
    10501 ttgcaaaact taacagaaac gtgcactttg atcccaacta ataccgtgta ctatcatgtg
    10561 tttggtttta agtactcatc cttcgtgttt cgttgtttgt tgtttgcctt gctgtacact
    10621 tggctcttgc gctctctcgc tctccgttgg agccggcttt ttgaatgcat gcctcgccct
    10681 gctcgcgccc agtcctcctc ccccgaaaag catgccgacc agcaaatgtt gcccttttgc
    10741 gtttgctgtc tttgcagaag caaaatcaat aactgagaaa tccaccacac tgctgctctt
    10801 cgtgttgaat tctcaacttc cgcagcaaag tgaaagacat ttcagtctct gacggcattt
    10861 cgtagcgcgc aaacactttc agttaaaagt tttgttactt tatagaagaa attttgagtt
    10921 tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag
    10981 tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat
    11041 atacagaccg ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac
    11101 aatatgatta tctttctagg gttaacgctg tcatgagacc ggatcctgac acggttatcc
    11161 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    11221 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    11281 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    11341 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    11401 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    11461 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    11521 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    11581 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    11641 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    11701 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    11761 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    11821 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    11881 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    11941 atccttttgt gatgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc
    12001 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
    12061 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
    12121 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
    12181 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
    12241 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
    12301 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
    12361 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
    12421 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
    12481 gtttttcgtg tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
    12541 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
    12601 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
    12661 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt
    12721 ttaaggcagt tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa
    12781 gcggatgaat ggcagaaatt cgatgataag ctgtcaaaca tgatgttacg cgccctgtag
    12841 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
    12901 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
    12961 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
    13021 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccag cgccctgata
    13081 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    13141 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
    13201 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
    13261 caaaatatta acgcttacaa ttt
//
