LOCUS       sequence-329290-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused 
            to ubiquitin (co-translationally processed).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, 
            Bachovchin DA, Wu H
  TITLE     Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome 
            formation by sequestering its active C-terminal fragment.
  JOURNAL   Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 
            10.1016/j.immuni.2021.04.024. Epub 2021 May 20.
  PUBMED    34019797
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2021.04.024"; journalName: "Immunity"; date: 
            "2021-07-13- 13"; volume: "54"; issue: "7"; pages: "1392-1404.e10"
COMMENT     Sequence Label: pDONR221 CARD8 Ub-S297
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..228
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 atgcagatct tcgtcaagac gttaaccggt aaaaccataa ctctggaagt tgaaccatcc
       61 gataccatcg aaaacgttaa ggctaaaatt caagacaagg aaggaattcc acctgatcaa
      121 caaagattga tctttgccgg taagcagctc gaggacggta gaacgctgtc tgattacaac
      181 attcagaagg agtcgacctt acatcttgtc ttaagactaa gaggtggttc tctgatgggc
      241 atcctgctgc ggatcgccag tgggactcgc ctctccatcc ccatcacttc caacacattg
      301 atctattatc acccccaccc cgaagatatt aagttccact tgtaccttgt ccccagcgac
      361 gccttgctaa caaaggcgat agatgatgag gaagatcgct tccatggtgt gcgcctgcag
      421 acttcgcccc caatggaacc cctgaacttt ggttccagtt atattgtgtc taattctgct
      481 aacctgaaag taatgcccaa ggagttgaaa ttgtcctaca ggagccctgg agaaattcag
      541 cacttctcaa aattctatgc tgggcagatg aaggaaccca ttcaacttga gattactgaa
      601 aaaagacatg ggactttggt gtgggatact gaggtgaagc cagtggatct ccagcttgta
      661 gctgcatcag cccctcctcc tttctcaggt gcagcctttg tgaaggagaa ccaccggcaa
      721 ctccaagcca ggatggggga cctgaaaggg gtgctcgatg atctccaaga caatgaggtt
      781 cttactgaga atgagaagga gctggtggag caggaaaaga cacggcagag caagaatgag
      841 gccttgctga gcatggtgga gaagaaaggg gacctggccc tggacgtgct cttcagaagc
      901 attagtgaaa gggaccctta cctcgtgtcc tatcttagac agcagaattt g
//
