LOCUS       sequence-329293-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pDONR221 for Gateway shuttling. Contains the CARD8 
            autoprocessing-deficient FIIND (L162M-P434, no stop codon) that 
            cannot bind DPP9 at site I (E274R).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, 
            Bachovchin DA, Wu H
  TITLE     Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome 
            formation by sequestering its active C-terminal fragment.
  JOURNAL   Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 
            10.1016/j.immuni.2021.04.024. Epub 2021 May 20.
  PUBMED    34019797
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2021.04.024"; journalName: "Immunity"; date: 
            "2021-07-13- 13"; volume: "54"; issue: "7"; pages: "1392-1404.e10"
COMMENT     Sequence Label: pDONR221 CARD8 FIIND E274R + S297A
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggggcctg aaggaaatgt ggatgttgag ttgattgata agagcacaaa cagatacagc
       61 gtttggttcc ccactgctgg ctggtatctg tggtcagcca caggcctcgg cttcctggta
      121 agggatgagg tcacagtgac gattgcgttt ggttcctgga gtcagcacct ggccctggac
      181 ctgcagcacc atgaacagtg gctggtgggc ggccccttgt ttgatgtcac tgcagagcca
      241 gaggaggctg tcgccgaaat ccacctcccc cacttcatct ccctccaagc aggtgaggtg
      301 gacgtctcct ggtttctcgt tgcccatttt aagaatagag ggatggtcct ggagcatcca
      361 gcccgggtgg agcctttcta tgctgtcctg gaaagcccca gcttcgccct gatgggcatc
      421 ctgctgcgga tcgccagtgg gactcgcctc tccatcccca tcacttccaa cacattgatc
      481 tattatcacc cccaccccga agatattaag ttccacttgt accttgtccc cagcgacgcc
      541 ttgctaacaa aggcgataga tgatgaggaa gatcgcttcc atggtgtgcg cctgcagact
      601 tcgcccccaa tggaacccct gaactttggt tccagttata ttgtgtctaa ttctgctaac
      661 ctgaaagtaa tgcccaagga gttgaaattg tcctacagga gccctggaga aattcagcac
      721 ttctcaaaat tctatgctgg gcagatgaag gaacccattc aacttgagat tactgaaaaa
      781 agacatggga ctttggtgtg ggatactgag gtgaagcca
//
