LOCUS       sequence-329302-        1614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9
            due to a mutation at site I (E274R) with sgRNA binding site 
            mutations .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, 
            Bachovchin DA, Wu H
  TITLE     Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome 
            formation by sequestering its active C-terminal fragment.
  JOURNAL   Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 
            10.1016/j.immuni.2021.04.024. Epub 2021 May 20.
  PUBMED    34019797
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2021.04.024"; journalName: "Immunity"; date: 
            "2021-07-13- 13"; volume: "54"; issue: "7"; pages: "1392-1404.e10"
COMMENT     Sequence Label: pDONR221 CARD8 PAM E274R
FEATURES             Location/Qualifiers
     source          1..1614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaaaaaa aggagtgtcc agaaaagagt agcagcagtg aggaagagct gccgagacgg
       61 gacagtggat ccagtaggaa catagatgca tccaaactca ttagactaca aggatcacgg
      121 aaactgttgg ttgacaatag catacgggaa ctgcaataca caaaaactgg aatttttttt
      181 caggctgagg cctgtgtgac aaatgatacg gtatacaggg agctaccctg tgtttctgag
      241 accctttgtg acatctcaca ttttttccaa gaagatgatg agacagaggc agagccatta
      301 ttgttccgtg ctgttcctga gtgtcaacta tctggggggg acattcccag tgtatcagaa
      361 gagcaggaat cttcagaggg acaagattca ggagacattt gctcagaaga gaatcaaata
      421 gtttcctctt atgcttctaa agtctgtttt gagatcgaag aagattataa aaatcgtcag
      481 tttctggggc ctgaaggaaa tgtggatgtt gagttgattg ataagagcac aaacagatac
      541 agcgtttggt tccccactgc tggctggtat ctgtggtcag ccacaggcct cggcttcctg
      601 gtaagggatg aggtcacagt taccatcgcc ttcggcagct ggagtcagca cctggccctg
      661 gacctgcagc accatgaaca gtggctggtg ggcggcccct tgtttgatgt cactgcagag
      721 ccagaggagg ctgtcgccga aatccacctc ccccacttca tctccctcca agcaggtgag
      781 gtggacgtct cctggtttct cgttgcccat tttaagaata gagggatggt cctggagcat
      841 ccagcccggg tggagccttt ctatgctgtc ctggaaagcc ccagcttctc tctgatgggc
      901 atcctgctgc ggatcgccag tgggactcgc ctctccatcc ccatcacttc caacacattg
      961 atctattatc acccccaccc cgaagatatt aagttccact tgtaccttgt ccccagcgac
     1021 gccttgctaa caaaggcgat agatgatgag gaagatcgct tccatggtgt gcgcctgcag
     1081 acttcgcccc caatggaacc cctgaacttt ggttccagtt atattgtgtc taattctgct
     1141 aacctgaaag taatgcccaa ggagttgaaa ttgtcctaca ggagccctgg agaaattcag
     1201 cacttctcaa aattctatgc tgggcagatg aaggaaccca ttcaacttga gattactgaa
     1261 aaaagacatg ggactttggt gtgggatact gaggtgaagc cagtggatct ccagcttgta
     1321 gctgcatcag cccctcctcc tttctcaggt gcagcctttg tgaaggagaa ccaccggcaa
     1381 ctccaagcca ggatggggga cctgaaaggg gtgctcgatg atctccaaga caatgaggtt
     1441 cttactgaga atgagaagga gctggtggag caggaaaaga cacggcagag caagaatgag
     1501 gccttgctga gcatggtgga gaagaaaggg gacctggccc tggacgtgct cttcagaagc
     1561 attagtgaaa gggaccctta cctcgtgtcc tatcttagac agcagaattt gtaa
//
