LOCUS       sequence-32940-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP112
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(23..56)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcgactctag aggatccccg ggataacttc gtatagcata cattatacga agttatgggc
       61 ccattatgaa tacgcacctc tatgtatttc cgagatacga ttactccagt tcctcttaca
      121 agaaatgcat aaaaatagtt acaattaatt agaacaagaa cttatttaga acacgttcac
      181 actgagtaag aactcttgtc ccttattagc cttgatagtg ctgaaaaaaa gaaaaaaaac
      241 aaaaaaaaga aataaaataa cggcaaacag caaaggccac agaaccgtat tcatgttact
      301 tctgcaatat caatcactta ctggcaagtg cgtataaatt aaacctattt ctttatcatc
      361 atatttactt atatctttaa cagattccaa accctaaagt gtccgaattt tcaatagggc
      421 gaacttgaag aataaccaag gtcaataata tatcttttag tataaccctg aaatttgccc
      481 tatagaaatc tagggtttct gtgtggtttc cgggtgagtc atacggcttt tttgaatttc
      541 tttttttgca gttgtctcta tcaatgaaaa tttcgaggaa gacgataagg ttaagataag
      601 tagataagag aatgatacga gataaagcac aaattagcag aaagaagagt ggttgcgaac
      661 agagtaaacc gaatcaggga atcccttttt gcaaaaacat caattatcct tttctttttt
      721 ttacgtatat atctggaaca gaaatatata agttactatt atacttatag ttggatccag
      781 tttttaatct gtcgtcaatc gaaagtttat ttcagagttc ttcagacttc ttaactcctg
      841 taaaa
//
