LOCUS       sequence-329405-       13088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Puromycin resistant FC31 integrase mediated RMCE cassette for 
            upgrading MiMic insertions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13088)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 13088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31
FEATURES             Location/Qualifiers
     source          1..13088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    49..118
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        146..597
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     promoter        1351..1802
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     polyA_signal    5538..5672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5562..5581)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5616..5635
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    5865..5934
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             8215..10032
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     protein_bind    9988..9999
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     repeat_region   10864..10926
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      complement(11055..11643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11135..11154)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11758..11860
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             11861..12520
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(11927..11946)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      12633..13061
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12720..12739)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12930..12951
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggatcc ggtctcagga ggaattggtc gacgatgtag gtcactgtct cgaagccgcg
       61 gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccacct cacccatctg
      121 gtccatcatg atgcgctgtc aggagctaga atcccaaaac aaactggtta ttgtggtagg
      181 tcatttgttt ggcagaaaga aaactcgaga aatttctctg gccgttattc gttattctct
      241 cttttctttt tgggtcgctc cctctctgca ctaatgctct ctcactctgt cacacagtaa
      301 acggcatact gctctcgttg gttcgagaga gcgcgcctcg aatgttcgcg aaaagagcgc
      361 cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt
      421 gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa
      481 tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc
      541 aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctcaa
      601 tactcgaatc catgtgggag tttattcttg acacagatat ttatgatata ataactgagt
      661 aagcttaaca taaggaggaa aacaaaatga ccgagtataa gcccacggtg cgtctggcca
      721 cccgtgatga tgtgccccgt gccgtgcgaa ccctggctgc tgccttcgcc gattacccag
      781 ccacccgtca caccgtggac ccagaccgtc acattgagcg tgtgaccgag ctccaggagc
      841 tgttcctgac acgcgtgggc ctggatatcg gcaaggtctg ggtggccgat gatggagctg
      901 ccgtggccgt gtggaccacc cccgagagcg tggaggctgg agccgtgttc gccgagattg
      961 gaccccgtat ggccgagctg agtggctccc gtctggctgc ccagcagcag atggagggcc
     1021 tgctggcccc acaccgtcca aaggagcccg tctggttcct ggccaccgtg ggcgtgagcc
     1081 ccgaccacca gggcaagggc ctgggcagtg ccgtggtgct gccaggagtg gaagctgccg
     1141 agcgtgctgg agtgccagcc ttcctggaaa ccagtgcccc acgcaatctg cccttctacg
     1201 agcgtctggg cttcaccgtg acggccgatg tggaggtgcc cgagggccca cgcacctggt
     1261 gtatgacccg taagccagga gcctaagctt ggcaataaaa agacagaata aaacgcacgg
     1321 gtgttgggtc gtttgttccg ctgtcaggag ctagaatccc aaaacaaact ggttattgtg
     1381 gtaggtcatt tgtttggcag aaagaaaact cgagaaattt ctctggccgt tattcgttat
     1441 tctctctttt ctttttgggt cgctccctct ctgcactaat gctctctcac tctgtcacac
     1501 agtaaacggc atactgctct cgttggttcg agagagcgcg cctcgaatgt tcgcgaaaag
     1561 agcgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc
     1621 aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta
     1681 aacaatctgc agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt
     1741 aaatcaactg caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact
     1801 ctcaatactc gaatccatgt gggagtttat tcttgacaca gatatttatg atataataac
     1861 tgagtaagct taacataagg aggaaaacaa aatggtgacc ggcggcatgg ccagcaagtg
     1921 ggatcaaaag ggcatggata ttgcctatga ggaggccgcc ttgggctaca aggagggcgg
     1981 cgtccccatt ggcggctgct tgatcaacaa caaggatggc agcgtgctgg gacgcggcca
     2041 caacatgcgc ttccagaagg gcagcgccac cttgcacggc gagatcagca ccctggagaa
     2101 ctgcggacgc ttggagggca aggtgtacaa ggataccacc ctgtacacca ccctgagccc
     2161 ctgcgatatg tgcaccggcg ccatcatcat gtacggcatc ccccgctgcg tggtgggcga
     2221 gaacgtgaac ttcaagagca agggcgagaa gtatctgcaa acccgcggac acgaggtggt
     2281 ggtggtggat gatgagcgat gcaaaaagat catgaagcag ttcatcgatg agcgccccca
     2341 ggattggttc gaggatattg gcgaggccag cgagcccttc aagaacgtgt atctgctgcc
     2401 ccagaccaac caactgctgg gcctgtacac catcatccgc aacaagaaca ccacccgccc
     2461 agatttcatc ttctattgtg accgcatcat ccgcctgctg gtcgaggagg gcctgaacca
     2521 cctgcccgtg cagaagcaga tcgtcgaaac cgataccaac gagaacttcg agggcgttag
     2581 cttcatgggc aagatctgcg gcgtgagcat cgtgcgtgcc ggcgagagca tggagcaggg
     2641 cctgcgcgat tgctgccgca gcgtgcgcat cggcaagatc ctgatccagc gcgacgagga
     2701 aaccgccctg cccaagctgt tttacgagaa gctgcccgag gatatttccg agcgctacgt
     2761 gttcctgctc gatcccatgc tggccaccgg cggcagcgcc atcatggcca ccgaggtgct
     2821 gatcaagcgc ggcgtgaagc ccgagcgcat ctactttctg aacctgattt gcagcaagga
     2881 gggcattgag aagtaccacg ccgccttccc ggaggtccgc atcgtgaccg gagccttgga
     2941 tcgcggattg gatgagaaca aatatctggt gcccggcctg ggcgatttcg gcgatcgcta
     3001 ctactgcgtg taagcttggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
     3061 tgttccgctg tcaggagcgt acgactagca cctgactgtc gagcccagtg gaaacccttg
     3121 aaatgccttt aagtcgagcc cagtggaaac ccttgaaatg cctttaagtc gagcccagtg
     3181 gaaacccttg aaatgccttt aagtcgagcc cagtggaaac ccttgaaatg cctttaagtc
     3241 gagcccagtg gaaacccttg aaatgccttt aagtcgagta gcagtcgagg taggtacgtc
     3301 atgcccctcc cgagctcgcc cggggatcga gcgcagcggt ataaaagggc gcggggtggc
     3361 tgagagcatc agttgtgaat gaatgttcga gccgagcaga cgtgccgctg ccttcgttaa
     3421 tatcctttga ataagccaac tttgaatcac aagacgcata ccaaaccaaa tgggacagga
     3481 ggaccaggag ctgctgatcc gcggaggctc caagcaccca agcgccgagc acctgaacaa
     3541 cggcgatagc ggagccgcca gccagtcgtg catcaatcag ggatttggcc aggccaagaa
     3601 ttacggaacc ctgctgcccc ccagcccccc agaggatagc ggaagcggat ccggacagct
     3661 ggccgagaat ctgacgtacg cctggcataa catggatatc ttcggcgccg tgaaccagcc
     3721 aggcagtggc tggcgtcagc tggtgaaccg cacccgcgga ctgttctgca atgagcgcca
     3781 tatcccagcc ccacgcaagc acctgctgaa gaatgtgtgc ggagtggcct accccggcga
     3841 gctgctggcc gtgatgggaa gtagtggcgc cggaaagacc acgctgctga acgccctggc
     3901 cttccgcagc ccccagggca ttcaggtgtc cccaagcggc atgcgcctgc tgaacggcca
     3961 gcccgtggat gccaaggaga tgcaggcccg ctgcgcctac gtgcagcagg acgatctgtt
     4021 catcggcagc ctgacagccc gcgagcatct gatctttcag gccatggtcc gcatgccccg
     4081 ccacctgaca taccgtcagc gcgtggcccg cgtggaccag gtcatccagg agctgagcct
     4141 gagcaagtgc cagcacacca ttattggcgt gccaggccgc gtgaagggcc tgagtggcgg
     4201 agagcgcaag cgcctggcct ttgccagcga ggccctgacc gatccccccc tgctgatttg
     4261 cgacgagccg accagcggcc tggatagctt caccgcccac tccgtggtgc aggtgctgaa
     4321 gaagctgagc cagaagggaa agacggtcat tctgacgatc caccagccca gctcggagct
     4381 gttcgagctg tttgataaga ttctgctgat ggccgaggga cgcgtggcct tcctgggaac
     4441 cccaagcgag gccgtggatt tcttcagcta tgtgggcgcc cagtgcccca cgaattacaa
     4501 ccccgccgac ttctatgtgc aagtgctggc cgtggtgcca ggacgcgaga ttgagagccg
     4561 cgatcgcatt gccaagatct gcgataactt cgccatctcc aaggtggccc gcgacatgga
     4621 gcagctgctg gccacgaaga acctggagaa gcccctggag cagcccgaga acggctatac
     4681 gtataaggct acctggttta tgcagtttcg cgccgtgctg tggcgcagct ggctgagtgt
     4741 gctgaaggag cccctgctgg tcaaggtccg cctgatccag accacgatgg tggccattct
     4801 gatcggcctg atcttcctgg gacagcagct gacgcaggtc ggagtgatga acatcaatgg
     4861 agccattttt ctgtttctga cgaacatgac gttccagaac gtgttcgcca ccatcaacgt
     4921 gttcaccagc gagctgcccg tgttcatgcg cgaggcccgc agccgcctgt accgctgcga
     4981 tacctacttc ctgggaaaga ccattgccga gctgccgctg ttcctgaccg tgcccctggt
     5041 gtttaccgcc attgcctacc ccatgattgg cctgcgtgcc ggagtgctgc atttctttaa
     5101 ttgcctggcc ctggtgaccc tggtggccaa cgtgtcgacc agcttcggct acctgatcag
     5161 ctgcgcctcc agcagcacca gcatggccct gagcgtggga ccccccgtga ttatcccctt
     5221 cctgctgttc ggcggattct ttctgaactc cggcagcgtg cccgtgtacc tgaagtggct
     5281 gagctacctg agctggtttc gctacgccaa tgagggcctg ctgatcaatc agtgggccga
     5341 tgtggagccc ggcgagatct cctgcaccag cagcaacacg acctgcccca gcagcggaaa
     5401 ggtcatcctg gagacactga atttcagtgc cgccgacctg ccactggatt atgtgggcct
     5461 ggccatcctg attgtgtcct tccgcgtgct ggcctatctg gctctgcgcc tgcgcgctcg
     5521 tcgcaaggag tgagcttgat ccagacatga taagatacat tgatgagttt ggacaaacca
     5581 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     5641 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     5701 ttcaggttca gggggaggtg tgggaggttt tttcagtatg gcaagcgctc gacgaattaa
     5761 caagcttcgc tctatctcgc tctctatctc tctcgatcgc tcgtgctaag cttaagaccg
     5821 tggagaacaa ggagaaccgc tgtcaggagc atcatgatgg accagatggg tgaggtggag
     5881 tacgcgcccg gggagcccaa gggcacgccc tggcacccgc accgcggctt cgagacagtg
     5941 acctacatcg tcgaccaatt ccgctgtcag gagctgcagc tggttgtagg tgcagttgcg
     6001 cttcttgcac ttggcgcact tgaggagatc ggtcttggtg ccctgcacgg tggccagctg
     6061 ggcatcgttg atggcctcct tgacgaactt ctcgcgcagc ttcttcatct catcgctggc
     6121 catctcctcc ggcgtcattt tggccagctg cttggcagtg acggcgccgc acataaagtt
     6181 gcctcgcaat ccaggatttt tggggtcctt cagattggcc acgcgtgacc taatgcgatt
     6241 cttgtacttc atatccgtgt tattgaactc ggagtaaatg gcatcctcca gttcggcggc
     6301 catttcctcc ggctcaccgc atccttcggg cacttcacca atcttcagtg cggtggccag
     6361 catttcgcgg catttgatgc ggaccgcatc tgtcatgccg ccggagggaa acgaggtctg
     6421 tgacgaggat gtcgagcctt tcttctcttt atccttggat gagctggatg aactggactt
     6481 atccttgccg gagattgacg acgacgactt ggcggcactg gtggacttcg aggcactgct
     6541 gttgttggag gagccctcct tagcggagct gttgttcggt gtggttggcg ccgggctggc
     6601 gaggaatcgt ttccagttct tgatcagcgt cttggccaga gcgatcacct cgtcgtcctt
     6661 gctactcttg cgcagctcgt ttacggtcat gccgatgcgc gttttggtca gaatgtcgag
     6721 attgatgtta agcgtttgca gggccttcag caggtccaga gcctgatcct gtccctgtac
     6781 ggtggatata tgtatatttg tgacatgatg cgcggcacgt tagctaatcc cgatacttac
     6841 tgtgccgtcg ctggccatct tgctcatctt cttttggatt cgaaacactt cctcctccac
     6901 gctcattttg gcccacttaa tttgatcaga ggttaggtta actggcgtcg gctgtcggct
     6961 ttaatcggtg ctgcgggtct gcgacagttg tttatgcgga agccgggcta tttctttttt
     7021 tccctccaat aaatgttgtt tactattttt ttcgtttctg tattcgcaga acgagttgac
     7081 agaacaccct aacgcgtttt agtgttaccg ggcggtggct tgtcaagaaa tgccttccca
     7141 tggctaatac aattcccaac tggaagtagt ttgggtttca gaagtcaaca gtgttaccgt
     7201 attttaatgt acatctggct tttaagcgag tacacacaca tagcattgcg atgtttttta
     7261 aacttgcact gcttgcaagc agggctccac tgtatttcaa tgaaagaaca gcgtgttggg
     7321 cgtttttgaa ttatactaat taccttcaat aacaattccc tatatatcac ttagttttaa
     7381 taaataaatc gtttgagtgt ggtctagaga tgatgtatta tgatgaagtg cggttgttgg
     7441 ggacaagcgg gatacgtctg cgggtcaaat cggcggtggg acatagtgag agggcaacgg
     7501 ctccaaaacc aagcgttgcg aacaatcacc aacgcgccct ggtacgcgag aaatgttgat
     7561 atcgcacatg acctcagcat ccaccttcag tttgagaaca aattaaatca ggcacatacc
     7621 agacaaccag gagaacactg aggaggcgtc atcaaagata tctttggaca cgtggcataa
     7681 acaagccaaa ctattatcta atcaatattt ttatacaaat tttgtatgtt tccatgttat
     7741 cttcgttgct cctgttagtt actacgtttc atagccctta aattggttgc ttaacgtaaa
     7801 ataaaattat attgtataaa aataaagagg aattcaccta taacaaacga aaaagtaaat
     7861 aaataagaac ttccccatgt taaaaatgcc accaccatct ttacatataa gtatgtacat
     7921 atgaatgaat cacttaggtt gcttgaatat tacaatttca ttaatagcta aatcacattt
     7981 gatgtgttag tggaaaacgg ctatatatat aaattatcga aattgtgaat atcgaattgc
     8041 gatagcacaa tgggaaattc caccactaga tttttggtac ttttaacaga tccttttcgg
     8101 ttttgcgttg cgcgaagtga tctgaactta tcaaagtttg taaggtaata cataaagtga
     8161 aaaagaatta atttgctctt gaaaggcagg ccaaattaaa aaaaaaatat caaaatggac
     8221 acctatgccg gtgcctacga cagacagtcc cgggagcggg aaaactccag tgcagccagc
     8281 cccgccacgc aacggtcagc taacgaggat aaggcggccg acctgcagag ggaggtggag
     8341 agggacggcg gcagattcag attcgtaggc cactttagcg aggcaccagg gactagtgcc
     8401 tttgggacag cagagaggcc agagttcgag agaatcctga atgagtgccg agcgggccgc
     8461 ctgaacatga tcatagttta cgatgtttcc cggttctcac ggctgaaggt tatggacgcc
     8521 atccctatcg taagtgagct gctggcactc ggggtgacta ttgttagcac tcaggaggga
     8581 gtgttccggc agggaaatgt catggacctg attcatttga ttatgcgcct ggacgccagc
     8641 cacaaagaaa gcagccttaa gagcgcgaaa atcttggaca caaagaatct tcagcgagag
     8701 cttggcggct acgtgggagg gaaggccccc tacgggttcg aactcgttag cgagacaaaa
     8761 gaaatcacca gaaacggccg aatggtcaac gtcgtcatta ataaactggc acattccaca
     8821 accccactga ctgggccatt tgaattcgaa cccgacgtga tccgttggtg gtggcgtgaa
     8881 attaagacgc acaagcatct gccctttaaa cctggatctc aggcagccat tcacccgggc
     8941 tccattactg ggctttgtaa gcggatggac gccgacgcag tcccaacgag gggggagaca
     9001 ataggcaaaa agactgcctc aagcgcgtgg gaccctgcca cagtcatgcg cattctccgg
     9061 gatccccgca ttgccgggtt cgccgcggag gtgatatata aaaagaagcc cgacggtaca
     9121 cccactacaa agattgaggg ctaccggatc cagcgcgacc ccattaccct gcggccagtc
     9181 gaactggatt gcgggcctat tatcgaaccg gcagaatggt acgaacttca ggcctggctg
     9241 gatggacggg ggagaggcaa agggctatct agaggccagg ctatcttgtc cgcgatggac
     9301 aaactttatt gcgaatgcgg cgccgtcatg acatccaaga gaggcgaaga gagcatcaaa
     9361 gatagttata gatgcagacg tcgcaaagtg gtcgatccct cagctcccgg acagcacgag
     9421 ggcacctgta atgtcagtat ggccgcatta gacaagttcg tcgcagaacg tatctttaac
     9481 aagatccgtc acgctgaagg agatgaagaa accctggctc tgttatggga ggccgccaga
     9541 cgatttggga agctgacaga ggctccagaa aaatctggcg aacgtgccaa cctggtagct
     9601 gagagagcgg atgcgctcaa tgcactggag gaactctacg aggatagggc agcgggcgcc
     9661 tacgatgggc cagtgggtcg caagcacttc cggaaacagc aggcagcact gacacttaga
     9721 caacagggcg ccgaggagag gctcgccgag ctagaggccg ctgaggcccc gaagctcccc
     9781 ctggatcagt ggttccctga ggatgccgac gctgatccca ccggacctaa aagctggtgg
     9841 gggcgagctt ccgtggacga taaacgggtt ttcgtcggcc tcttcgtgga caagatcgtg
     9901 gtgactaaga gcaccactgg cagagggcaa ggcacaccaa tcgagaagcg cgcatctatc
     9961 acttgggcta agcctcctac cgacgacgat gaggatgacg cacaggacgg gacagaggat
    10021 gtagcagcct aagcttaatg tatggacata gatttcaaat aattaaatgt aatgcagtaa
    10081 ttgatgtaat tagttaaata agttagatat taataacata ttaattatat gtattataac
    10141 gcatataata ataaaatgca tatttaggat atgcaagcca ttcgaatttt ctattttaat
    10201 ttccttttac aaagaaatgt ataacaaaat ataatttgaa aaaatgttct ggctctaatt
    10261 cgatttcttt taagtatttt gtgaactgct tttaataacg agcggttgca aaacttaaca
    10321 gaaacgtgca ctttgatccc aactaatacc gtgtactatc atgtgtttgg ttttaagtac
    10381 tcatccttcg tgtttcgttg tttgttgttt gccttgctgt acacttggct cttgcgctct
    10441 ctcgctctcc gttggagccg gctttttgaa tgcatgcctc gccctgctcg cgcccagtcc
    10501 tcctcccccg aaaagcatgc cgaccagcaa atgttgccct tttgcgtttg ctgtctttgc
    10561 agaagcaaaa tcaataactg agaaatccac cacactgctg ctcttcgtgt tgaattctca
    10621 acttccgcag caaagtgaaa gacatttcag tctctgacgg catttcgtag cgcgcaaaca
    10681 ctttcagtta aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa
    10741 taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat
    10801 ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa
    10861 acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt
    10921 ctagggttaa cgctgtcatg agaccggatc ctgacacggt tatccacaga atcaggggat
    10981 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    11041 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    11101 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    11161 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    11221 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    11281 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    11341 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    11401 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    11461 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    11521 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    11581 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    11641 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    11701 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttgtgatga
    11761 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
    11821 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
    11881 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
    11941 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
    12001 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
    12061 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
    12121 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
    12181 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
    12241 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtgtcagc
    12301 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
    12361 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
    12421 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
    12481 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
    12541 gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag
    12601 aaattcgatg ataagctgtc aaacatgatg ttacgcgccc tgtagcggcg cattaagcgc
    12661 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    12721 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    12781 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    12841 acttgattag ggtgatggtt cacgtagtgg gccagcgccc tgatagacgg tttttcgccc
    12901 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    12961 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    13021 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
    13081 tacaattt
//
