LOCUS       sequence-329406-       10208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. 
            Enhancer grammar GB20 entry point for custom enhancers. 
            Cut-and-paste cloning can be used. Purple-white bacteria colony 
            screening..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pPuroR-MCS-GAL4-MiniWhite
FEATURES             Location/Qualifiers
     source          1..10208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    123..192
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        220..671
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     619..640
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     promoter        1425..1443
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1425..1442
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1454..1471)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      2279..2373
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    2380..2436
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(2498..2516)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2679..3119
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    3520..3654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3544..3563)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3598..3617
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3742..3760)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4212..4283,4685..4958,5033..5687,5749..6064,6268..6399,
                     6470..7084)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     rep_origin      complement(7851..8439)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7931..7950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8907..9698
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9753..10181
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9840..9859)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10050..10071
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggatca gcgcagctgc ccgggtctag agtcgacgca tgcctcctga cagcgagatg
       61 tcctaatatg gacatcctct ccggatccca gggaggaatt ggtcgacgat gtaggtcact
      121 gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc
      181 acctcaccca tctggtccat catgatgcgc tgtcaggagc tagaatccca aaacaaactg
      241 gttattgtgg taggtcattt gtttggcaga aagaaaactc gagaaatttc tctggccgtt
      301 attcgttatt ctctcttttc tttttgggtc gctccctctc tgcactaatg ctctctcact
      361 ctgtcacaca gtaaacggca tactgctctc gttggttcga gagagcgcgc ctcgaatgtt
      421 cgcgaaaaga gcgccggagt ataaatagag gcgcttcgtc tacggagcga caattcaatt
      481 caaacaagca aagtgaacac gtcgctaagc gaaagctaag caaataaaca agcgcagctg
      541 aacaagctaa acaatctgca gtaaagtgca agttaaagtg aatcaattaa aagtaaccag
      601 caaccaagta aatcaactgc aactactgaa atctgccaag aagtaattat tgaatacaag
      661 aagagaactc tcaatactcg aatccatgtg ggagtttatt cttgacacag atatttatga
      721 tataataact gagtaagctt aacataagga ggaaaacaaa atgaccgagt ataagcccac
      781 ggtgcgtctg gccacccgtg atgatgtgcc ccgtgccgtg cgaaccctgg ctgctgcctt
      841 cgccgattac ccagccaccc gtcacaccgt ggacccagac cgtcacattg agcgtgtgac
      901 cgagctccag gagctgttcc tgacacgcgt gggcctggat atcggcaagg tctgggtggc
      961 cgatgatgga gctgccgtgg ccgtgtggac cacccccgag agcgtggagg ctggagccgt
     1021 gttcgccgag attggacccc gtatggccga gctgagtggc tcccgtctgg ctgcccagca
     1081 gcagatggag ggcctgctgg ccccacaccg tccaaaggag cccgtctggt tcctggccac
     1141 cgtgggcgtg agccccgacc accagggcaa gggcctgggc agtgccgtgg tgctgccagg
     1201 agtggaagct gccgagcgtg ctggagtgcc agccttcctg gaaaccagtg ccccacgcaa
     1261 tctgcccttc tacgagcgtc tgggcttcac cgtgacggcc gatgtggagg tgcccgaggg
     1321 cccacgcacc tggtgtatga cccgtaagcc aggagcctaa gcttggcaat aaaaagacag
     1381 aataaaacgc acgggtgttg ggtcgtttgt tccgctgtca ggagatttag gtgacactat
     1441 agacttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg tcgggagaga
     1501 gaccaatcca tgtgggagtt tattcttgac acagatattt atgatataat aactgagtaa
     1561 gcttaacata aggaggaaaa caaaatggcg agcttggtta agaaagatat gtgtgttaag
     1621 atgacgatgg aaggtactgt gaacggttat cactttaagt gcgttggcga gggtgaaggc
     1681 aagccgttcg agggcacgca gaacatgcgc attcgtgtca ccgagggcgg tccgctgcct
     1741 tttgcattcg acatcctggc cccgtgctgt atgtacggct ctaagacctt cattaaacac
     1801 gtgagcggta tcccggatta ctttaaagag tcctttccag agggcttcac ttgggaacgt
     1861 acccagattt ttgaggacgg tggtgttctg accgcgcacc aagacaccag cctggaaggt
     1921 aattgcctga tctataaagt gaaggttctg ggtaccaatt tcccggcgaa tggtccggtg
     1981 atgcaaaaga aaaccgcggg ttgggagccg tgcgtcgaga tgctgtatcc gcgtgacggc
     2041 gtcttgtgtg gtcagagctt gatggcgctg aagtgcaccg atggcaatca tctgaccagc
     2101 cacctgcgca cgacgtatcg tagccgtaaa ccgagcaacg ccgttaacat gccggagttc
     2161 cattttggtg accatcgcat cgaaatcctg aaagctgagc agggcaaatt ctacgaacaa
     2221 tacgaatcgg ctgtcgcacg ttacagcgat gtgccggaaa aagcgacgta atgagcttga
     2281 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     2341 ggttgccgcc gggcgttttt tattggtgag aattacttca agcttgcatg cctgcaggtc
     2401 gactctagag gatccccggg taccgagctc gaattcggtc tcttcccgct tgtgtgaaat
     2461 accgcacaga tgcgtaagga gaaaataccg catcaggcct atagtgagtc gtattatccc
     2521 gagctcgccc ggggatcgag cgcagcggta taaaagggcg cggggtggct gagagcatca
     2581 gttgtgaatg aatgttcgag ccgagcagac gtgccgctgc cttcgttaat atcctttgaa
     2641 taagccaact ttgaatcaca agacgcatac caaaccaaat gaagctgctg agtagtattg
     2701 aacaagcgtg tgacatatgc cgacttaaga agctgaagtg cagcaaggaa aagccgaagt
     2761 gcgccaaatg tcttaagaac aattgggagt gccggtactc gccaaagacg aagcgaagcc
     2821 ctttgacacg cgctcatttg actgaagtgg agagtcgcct ggagcgcctt gagcagttgt
     2881 tcctcctcat ttttccccgt gaggacctcg acatgatact gaagatggat agtctccaag
     2941 atattaaggc cctgctcact ggcctgtttg ttcaggataa cgtcaacaag gatgccgtga
     3001 cggaccgcct tgcctcggtg gagacagata tgccacttac cttgcggcag catcgcatct
     3061 cggctaccag cagtagtgaa gaaagttcca ataagggtca acggcaactg acggtatcga
     3121 gtggcggagg ttccggtggc ggcagcggcg ggggttcggg aggtggctcg gccaacttca
     3181 accagagtgg taacattgcg gacagctcct tgagcttcac gtttaccaac agctccaacg
     3241 gtccaaactt gatcacgact cagacaaact cccaggcatt gtcccagccc attgccagct
     3301 cgaatgtgca tgataacttt atgaataatg aaattactgc ctccaagatt gacgacggca
     3361 acaacagcaa gcccctttcc ccaggctgga ctgaccagac ggcctacaat gctttcggta
     3421 tcaccactgg catgttcaac accacaacga tggacgacgt atataactat ctttttgatg
     3481 atgaggatac tccaccgaac ccaaagaagg agtaggcttg atccagacat gataagatac
     3541 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     3601 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     3661 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttcgctg
     3721 tcaggaggaa ttctagtatg tatgtaagtt aataaaaccc atttttgcgg aaagtagata
     3781 aaaaaaacat tttttttttt tactgcactg gatatcattg aacttatctg atcagtttta
     3841 aatttacttc gatccaaggg tatttgatgt accaggttct ttcgattacc tctcactcaa
     3901 aatgacattc cactcaaagt cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc
     3961 cgtcactttc gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg ttacgtagcg
     4021 tcaatgtccg ccttcagttg cattttgtca gcggtttcgt gacgaagctc caagcggttt
     4081 acgccatcaa ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt atttttgttt
     4141 gttttttgag tgattggggt ggtgattggt tttgggtggg taagcagggg aaagtgtgaa
     4201 aaatcccggc aatgggccaa gaggatcagg agctattaat tcgcggaggc agcaaacacc
     4261 catctgccga gcatctgaac aatgtgagta gtacatgtgc atacatctta agttcacttg
     4321 atctatagga actgcgattg caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca
     4381 aaaatcccaa accgcaattg cacaaacaaa tagtgacacg aaacagatta ttctggtagc
     4441 tgttctcgct atataagaca atttttgaga tcatatcatg atcaagacat ctaaaggcat
     4501 tcattttcga ctatattctt ttttacaaaa aatataacaa ccagatattt taagctgatc
     4561 ctagatgcac aaaaaataaa taaaagtata aacctacttc gtaggatact tcggggtact
     4621 ttttgttcgg ggttagatga gcataacgct tgtagttgat atttgagatc ccctatcatt
     4681 gcagggtgac agcggagcgg cttcgcagag ctgcattaac cagggcttcg ggcaggccaa
     4741 aaactacggc acgctccggc cacccagtcc gccggaggac tccggttcag ggagcggcca
     4801 actagccgag aacctcacct atgcctggca caatatggac atctttgggg cggtcaatca
     4861 gccgggctcc ggatggcggc agctggtcaa ccggacacgc ggactattct gcaacgagcg
     4921 acacataccg gcgcccagga aacatttgct caagaacggt gagtttctat tcgcagtcgg
     4981 ctgatctgtg tgaaatctta ataaagggtc caattaccaa tttgaaactc agtttgcggc
     5041 gtggcctatc cgggcgaact tttggccgtg atgggcagtt ccggtgccgg aaagacgacc
     5101 ctgctgaatg cccttgcctt tcgatcgccg cagggcatcc aagtatcgcc atccgggatg
     5161 cgactgctca atggccaacc tgtggacgcc aaggagatgc aggccaggtg cgcctatgtc
     5221 cagcaggatg acctctttat cggctcccta acggccaggg aacacctgat tttccaagcc
     5281 atggtgcgga tgccacgaca tctgacctat cggcagcgag tggcccgcgt ggatcaggtg
     5341 atccaggagc tttcgctcag caaatgtcag cacacgatca tcggtgtgcc cggcagggtg
     5401 aaaggtctgt ccggcggaga aaggaagcgt ctggcattcg cctccgaggc tctaaccgat
     5461 ccgccgcttc tgatctgcga tgagcccacc tccggactgg actcctttac cgcccacagc
     5521 gtcgtccagg tgctgaagaa gctgtcgcag aagggcaaga ccgtcatcct gaccattcat
     5581 cagccgtctt ccgagctgtt tgagctcttt gacaagatcc ttctgatggc cgagggcagg
     5641 gtagctttct tgggcactcc cagcgaagcc gtcgacttct tttcctagtg agttcgatgt
     5701 gtttattaag ggtatctagt attacataac atctcaactc ctatccagcg tgggtgccca
     5761 gtgtcctacc aactacaatc cggcggactt ttacgtacag gtgttggccg ttgtgcccgg
     5821 acgggagatc gagtcccgtg atcggatcgc caagatatgc gacaattttg ccattagcaa
     5881 agtagcccgg gatatggagc agttgttggc caccaaaaat ctggagaagc cactggagca
     5941 gccggagaat gggtacacct acaaggccac ctggttcatg cagttccggg cggtcctgtg
     6001 gcgatcctgg ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac ttattcagac
     6061 aacggtgagt ggttccagtg gaaacaaatg atataacgct tacaattctt ggaaacaaat
     6121 tcgctagatt ttagatagaa ttgcctgatt ccacaccctt cttagttttt ttcaatgaga
     6181 tgtatagttt atagttttgc agaagataaa taaatttcat ttaactcgcg aatattaatg
     6241 agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct catctttttg
     6301 ggccaacaac tcacgcaagt gggtgtgatg aatatcaacg gagccatctt cctcttcctg
     6361 accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcatgt ttagaataca
     6421 tttgcatttc aataatttac taactttcta atgaatcgat tcgatttagg tgttcacctc
     6481 agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg acacatactt
     6541 tctgggcaaa acgattgccg aattgccgct ttttctcaca gtgccactgg tcttcacggc
     6601 gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca actgcctggc
     6661 gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat cctgcgccag
     6721 ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat tcctgctctt
     6781 tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct
     6841 ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc
     6901 gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct
     6961 ggagacactt aacttctccg ccgccgatct gccgctggac tacgtgggtc tggccattct
     7021 catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga
     7081 gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta ttaccatcgt
     7141 gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca
     7201 agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg
     7261 atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat
     7321 cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc
     7381 ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg
     7441 ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc
     7501 gcgattcggt tcgctcaaat ggttccgagt ggttcatttc ggctcaatag aaattagtaa
     7561 taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt ccctcacagc
     7621 tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga cctgttcgga
     7681 gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct tgtgtagatg
     7741 catcgcttga cacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7801 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7861 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7921 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7981 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8041 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8101 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8161 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8221 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8281 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8341 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8401 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8461 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8521 tcaaaaagga tcttcaccta gatccttttg tgaccagcca ggacagaaat gcctcgactt
     8581 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     8641 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     8701 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     8761 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     8821 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     8881 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     8941 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     9001 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     9061 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     9121 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     9181 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     9241 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     9301 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     9361 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     9421 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     9481 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtagct gccgactggg
     9541 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     9601 ttggacaaga agaagatcgt ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     9661 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatc actagaccaa tgttacacat
     9721 atatacttta gattgattta aaacttcatg ttacgcgccc tgtagcggcg cattaagcgc
     9781 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9841 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9901 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9961 acttgattag ggtgatggtt cacgtagtgg gccagcgccc tgatagacgg tttttcgccc
    10021 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    10081 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    10141 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
    10201 tacaattt
//
