LOCUS       sequence-329419-        9489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutant E141D ATG5, HA-ATG12 ATG16 in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Kim M, Sandford E, Gatica D, Qiu Y, Liu X, Zheng Y, Schulman BA, Xu 
            J, Semple I, Ro SH, Kim B, Mavioglu RN, Tolun A, Jipa A, Takats S, 
            Karpati M, Li JZ, Yapici Z, Juhasz G, Lee JH, Klionsky DJ, 
            Burmeister M
  TITLE     Mutation in ATG5 reduces autophagy and leads to ataxia with 
            developmental delay
  JOURNAL   Elife. 2016 Jan 26;5. pii: e12245. doi: 10.7554/eLife.12245.
  PUBMED    26812546
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-01-26"; volume: "5. pii: e12245. doi"; pages: " 
            10.7554/eLife.12245"
COMMENT     Sequence Label: pRS416 ATG5-E141D-HA-ATG12-ATG16
FEATURES             Location/Qualifiers
     source          1..9489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        319..423
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(424..492,493..1284)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(642..661)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1455..2043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1944..1963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2197..2214
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2331..2352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2367..2372,2373..2390,2391..2397)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2405..2421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2410..2432
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2429..2445
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2429..2445
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2464..2484
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2466..2484
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3811..3827
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3811..3827
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5595..5611)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5596..5612)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             6109..6198
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(7215..7234)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7216..7234)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7244..7261)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7244..7260)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7253..7275)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7401..7856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7488..7507)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7698..7719
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7987..8790)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8791..9006)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9030..9049)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9149..9171
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9209..9227)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(9268..9489,1..282)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 gattaaatag atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt
       61 gtgtggtctt ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag
      121 agcaagataa aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa
      181 acaaaaacta ttttttcttt aatttctttt tttactttct atttttaatt tatatattta
      241 tattaaaaaa tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc
      301 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      361 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      421 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      481 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      541 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      601 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      661 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      721 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      781 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
      841 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
      901 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
      961 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1021 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1081 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1141 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1201 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1261 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1321 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1381 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1441 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1501 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1561 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1621 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1681 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1741 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1801 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     1861 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     1921 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1981 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2041 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2101 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2161 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2221 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2281 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     2341 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     2401 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     2461 cgcgcaatta accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc
     2521 tctatatctg gatgttgcaa aagaagcgat tttagaccat atccctttat tgtcattatt
     2581 cgagttcacg ctatcttgaa aatccgttgt cggactactc cttccttgtc cattaggata
     2641 ttctgcaatt cttcccgtgt ttggctcatc gtcggcagtg gaattccacc agggtttcca
     2701 tctgttttgt tgtcgtggac gttggggtgg gacttcctgc tggtcctctc gtatctgctg
     2761 ctgctcttgt gtttgatgct gcaactgttt atcctgtttc tgctgcacaa catccattat
     2821 aatagcgttt tatatgttgt gctaaattgc tactacactt gctcgctttt acttattctg
     2881 tttgtagttg acctttattt aacgttgatt tttgttatta acctgcgcga atctgtatta
     2941 agggcgcata ttccatgggc aatttcatta taacagaaag gaagaaagca aaggaggaaa
     3001 gaagcaatcc acaaacagat agcatggatg atttgttaat tcgtagacta acagatcgaa
     3061 acgacaagga ggcccattta aatgagttgt ttcaagataa tagtggcgcc attggtggca
     3121 acattgtcag ccatgacgat gctttattga atacactggc aattctacaa aaagaactga
     3181 aaagtaagga gcaagaaata cgaagattga aagaagttat tgcgttgaaa aataagaata
     3241 cggaaaggtt gaatgacgaa ttgattagtg gaaccattga aaataatgtt ttacaacaaa
     3301 aactctcgga tctgaaaaaa gaacattccc agctagttgc gagatggcta aaaaagacag
     3361 agaaagagac agaagccatg aacagcgaaa tagatggaac gaaatgatgc atcagttgtc
     3421 aaatcgtcac aaaatgcata caaaagaaaa taaaatcatt gtggtgcata tacatggaca
     3481 gaatatgaaa cgccaaagtg tttgcaataa agaggtgaaa atatgttaaa catgctatag
     3541 agagtacacc tgcaagttaa gaaaatgcca ttcgtgtttt tatctactat aatgtaatgt
     3601 gacagctgtt ttaaaccttt cttaaggtcg tttgtgcgag aacaggcttt ttttttggag
     3661 aatgtatctt gagagaaact caatagtaat aactggatgc ataaagaaaa tttcatatga
     3721 atcactacaa atctttggcg ttagaattct attgggtaaa ttgaactttg tagctttttg
     3781 atggtaaact tggcggtttt gcgcggccgc tctagaacta gtggatccgg acaataatcg
     3841 gcgaagggta tcttcattgc tagtagagga tggggggaat cctacagcgt aggtaccgga
     3901 taacagagta actggtaaaa acggcaaaaa atactgtaag gaagttgtct atatatgtac
     3961 atatggatat tgcggtataa taaaaaagct ttaaaattat aaagaaaccc gacttttatc
     4021 acatataatt atattataaa aaaataatgg catctgtatg aactcgcgat taagctcact
     4081 tttttctaat gatcgaagtt ttgtccggtt atccaactgt ggcaagaaca tcgcgaggcg
     4141 gagctaaaca cagaattttt gtagcgcgat gagaaacata acgcctcttt agataaatta
     4201 tcatcacctt ttttacgcta aataatgaag tagcatgctc agaagtgcga atgaattttt
     4261 gttcttttgg ttctagaaga acggagatag gaaacctatg atgtaagtat gaatgacatt
     4321 aaacaattac tttggaatgg tgagcttaat gtgctggtat cgatcgatcc ttcatttttg
     4381 atgaaaggaa gtccaagaga aattgcggtg ctacgaataa gggtaccaag ggaaacatat
     4441 ttagtcaatt atatgcccct catttggaac aagattaaaa gctttctttc ttttgaccca
     4501 ttgaccgaca gtgagaagta tttctggttt gagcataata agacgcctat tccgtggaat
     4561 tacccagtag gtgttttgtt tgactgccta gccggaaaaa gcgctacttt taccacctct
     4621 tttgagaacc aggtaaagga tgttctcact tttttgagaa ttcacttggt tatgggcgat
     4681 tcgttgccac caactatcat tcccatcgca tctagcaaaa cgcaagcgga taagttttgg
     4741 tttcaccaat ggaaacaagt ttgcttcata ctgaatggtt cctcaaaagc tattatgtct
     4801 ttatcggtca acgaagctcg aaaattttgg ggcagtgtta ttacgagaaa tttccaagat
     4861 ttcattgaaa tatctaacaa gataagttca tcaagaccgc gacatatacc gctcatcatt
     4921 caaacctcaa ggacatcagg aacttttcga atatcacaac caactatcag tatgactgga
     4981 gtcaatccta cgttgaagga tattgaaggc gatattctgg acgtaaagga aggaatcaat
     5041 ggtaatgatg tcatggttat ttgtcaagga attgaaattc cttggcatat gctcctgtat
     5101 gatttgtatt ctaaattgcg aagttttgat ggctttttgt acataactct tgttcctata
     5161 aaaggcggcg ataaagcttc ctctgagctc taagagctgt tatatacgca tttaaaagtg
     5221 tcattcaaat atcgcagaaa ataacttgga cgttgaaaat agggccacat ttatacggct
     5281 gcaagaaaac ttttactctc tttctaccaa gtttgttact gcaattaaat aatctttatc
     5341 atgggatagc gaaactaaaa actcatgagt ggaaaagaga gaatcgtagt ttttcgagag
     5401 ctgttgatac tttggatact ttattccaaa catcttatca aactctaact gaggtacacc
     5461 tgtatcggtt tgtgttttat atagcattcc agcgtacagt acttgggcag gaggtagatc
     5521 atgctttgaa acacagcaac acaacgcttt caatgaggat cccccgggct gcaggaattc
     5581 gatatcaagc ttatcgatac cgtcgacctc gagattatcc tttgacaaga acttcaataa
     5641 tcccttaggc agatcccaac cgatttcacc agtagtactc ggattatcct ctaacaatgt
     5701 attcaaagtt tcaatgaatc tcgtcttttc ttcatagctc ccctcttggt taacttgctc
     5761 gccatatata tcaatcaatg ttaccaactc aagaagatcc tccttctgag ttttgaacgc
     5821 atcagtaacc tcaaaaagta tgtcatcaat tggttccatt agtgtctgag tatcgtattg
     5881 aatgtctagg gtgattttct ttttcagtgt ttatttgtta ataatgttca ccggcaatta
     5941 gtctttattt ttttccttta cattgtcaaa tttaaagaat cgtacttatg aaggatgccc
     6001 gcttagtgtt tgacagaaag ctacttgagc tgaagaaata aacgtacatc cctaactgta
     6061 tattctacag tagagtgaac caatgacagt atgtctagat ctatctttta cccatacgat
     6121 gttcctgact atgcgggcta tccctatgac gtcccggact atgcaggatc ctatccatat
     6181 gacgttccag attacgctgc tcagtgcaga tctagaagta ggatcctaga gagcgaaaat
     6241 gaaacagaaa gtgacgaaag ctccatcata tccacaaata atggaacggc aatggaaaga
     6301 tccagaaata atcaagaatt aagatcatct cctcataccg ttcaaaatag attggaactt
     6361 tttagcagga gattgtctca gcttggtttg gcgagtgaca tttctgtcga ccagcaagtt
     6421 gaagattcct ctagtggcac ttatgaacag gaagagacaa tcaaaacgaa tgcacaaaca
     6481 agcaaacaaa aaagccataa agacgaaaaa aacatacaaa agatacagat aaaatttcag
     6541 cccattggtt ctattgggca gttaaaacca tctgtttgta aaatatcaat gtcacagtct
     6601 tttgcaatgg ttattttatt tcttaagaga cggctgaaaa tggaccatgt ttattgttat
     6661 ataaataatt cgtttgcgcc aagtccgcag caaaatattg gtgaactttg gatgcaattc
     6721 aagactaatg atgagcttat tgtaagttat tgtgcatccg tagcgtttgg ttaaataccc
     6781 gggcgctgga atggtctaag aagaaaacct acagtcgatc aaaaatcccg tacgatatta
     6841 aatagagtag ttaacgtact tgagcgttat aaactcacat tttcactcaa gaagaaaatg
     6901 gagaatggag acatggtaag tgaaagcatt aaggaagaaa cacagttagc acgtatttca
     6961 tcgaaagtgt ttgattactt tctatttggc atttaaatat acatgggaac ataactagat
     7021 aaaatatacg ccatatatag tacagctagt attttcagat gtcataaaat ataattaacg
     7081 agtaaaaatt actttttttg ggatgggggt agggtttctt cttctaggac aaccaacaaa
     7141 tctgatgcgt caacactttc accatcctta atgaaaacgt ctttaactcg agggggggcc
     7201 cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     7261 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     7321 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     7381 gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7441 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7501 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7561 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7621 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7681 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7741 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7801 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg
     7861 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg gtaataactg
     7921 atataattaa attgaagctc taatttgtga gtttagtata catgcattta cttataatac
     7981 agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta
     8041 acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat
     8101 aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc
     8161 tcccttgtca tctaaaccca caccgggtgt cataatcaac caatcgtaac cttcatctct
     8221 tccacccatg tctctttgag caataaagcc gataacaaaa tctttgtcgc tcttcgcaat
     8281 gtcaacagta cccttagtat attctccagt agatagggag cccttgcatg acaattctgc
     8341 taacatcaaa aggcctctag gttcctttgt tacttcttct gccgcctgct tcaaaccgct
     8401 aacaatacct gggcccacca caccgtgtgc attcgtaatg tctgcccatt ctgctattct
     8461 gtatacaccc gcagagtact gcaatttgac tgtattacca atgtcagcaa attttctgtc
     8521 ttcgaagagt aaaaaattgt acttggcgga taatgccttt agcggcttaa ctgtgccctc
     8581 catggaaaaa tcagtcaaga tatccacatg tgtttttagt aaacaaattt tgggacctaa
     8641 tgcttcaact aactccagta attccttggt ggtacgaaca tccaatgaag cacacaagtt
     8701 tgtttgcttt tcgtgcatga tattaaatag cttggcagca acaggactag gatgagtagc
     8761 agcacgttcc ttatatgtag ctttcgacat gatttatctt cgtttcctgc aggtttttgt
     8821 tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac tacatatgcg
     8881 tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac
     8941 cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga
     9001 attgaaaagg tggtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     9061 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     9121 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     9181 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9241 gtcatgataa taatggtttc ttagacggat cgcttgcctg taacttacac gcgcctcgta
     9301 tcttttaatg atggaataat ttgggaattt actctgtgtt tatttatttt tatgttttgt
     9361 atttggattt tagaaagtaa ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa
     9421 taaacaaagg tttaaaaaat ttcaacaaaa agcgtacttt acatatatat ttattagaca
     9481 agaaaagca
//
