LOCUS       sequence-32943-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral shRNA expression vector containing scrambled 
            shRNA sequence. Uses blasticidin for selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, 
            Martin-Belmonte F, Mostov KE
  TITLE     A molecular network for de novo generation of the apical surface and
            lumen.
  JOURNAL   Nat Cell Biol. 2010 Nov;12(11):1035-45. doi: 10.1038/ncb2106. Epub 
            2010 Oct 3.
  PUBMED    20890297
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2106"; 
            journalName: "Nat Cell Biol"; date: "2010-11"; volume: "12"; issue: 
            "11"; pages: "1035-45"
COMMENT     Sequence Label: pLKO.1-blast-SCRAMBLE
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    111..228
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        277..760
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     706..724
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 aaggacgaaa caccggccta aggttaagtc gccctcgctc gagcgagggc gacttaacct
       61 taggtttttg aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga
      121 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      181 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
      241 agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca
      301 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
      361 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
      421 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
      481 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
      541 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
      601 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
      661 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
      721 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaa
//
