LOCUS       sequence-32944-e         496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, 
            Martin-Belmonte F, Mostov KE
  TITLE     A molecular network for de novo generation of the apical surface and
            lumen.
  JOURNAL   Nat Cell Biol. 2010 Nov;12(11):1035-45. doi: 10.1038/ncb2106. Epub 
            2010 Oct 3.
  PUBMED    20890297
REFERENCE   2  (bases 1 to 496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2106"; 
            journalName: "Nat Cell Biol"; date: "2010-11"; volume: "12"; issue: 
            "11"; pages: "1035-45"
COMMENT     Sequence Label: pLKO.1-Blast-cfCdc42_2
FEATURES             Location/Qualifiers
     source          1..496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     101..120
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    271..388
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
       61 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      121 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      181 gattacgacc gctgagttac tcgagtaact cagcggtcgt aatctttttg aattctcgac
      241 ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
      301 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
      361 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
      421 ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
      481 anggacgcgg ctgctc
//
