LOCUS       sequence-329477-        3054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector encoding spike (S1) RBD domain from 
            SARS-CoV-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Gkekas I, Katsamakas S, Mylonas S, Fotopoulou T, Magoulas GEpsilon, 
            Tenchiu AC, Dimitriou M, Axenopoulos A, Rossopoulou N, Kostova S, 
            Wanker EE, Katsila T, Papahatjis D, Gorgoulis VG, Koufaki M, 
            Karakasiliotis I, Calogeropoulou T, Daras P, Petrakis S
  TITLE     AI Promoted Virtual Screening, Structure-Based Hit Optimization, and
            Synthesis of Novel COVID-19 S-RBD Domain Inhibitors.
  JOURNAL   J Chem Inf Model. 2024 Nov 13. doi: 10.1021/acs.jcim.4c01110.
  PUBMED    39535926
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Chem Inf 
            Model. 2024 Nov 13. doi: 10.1021/acs.jcim.4c01110."
COMMENT     Sequence Label: pDONR221-SARS-CoV-2 S1 RBD
FEATURES             Location/Qualifiers
     source          1..3054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(152..179)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     238..260
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     252..269
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     253..269
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    285..384
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1063..1162)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1179..1198)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1180..1198)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1203..1219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1203..1219)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1321..1340)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1332..2141
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1399..1418)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2288..2876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2777..2796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3030..3047
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
       61 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
      121 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
      181 cttagtttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
      241 agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata atgattttat
      301 tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc
      361 caactttgta caaaaaagca ggctggatgc gcgtgcagcc caccgagtct atcgtgagat
      421 tcccaaacat caccaacctg tgcccattcg gagaagtgtt caacgctacc aggttcgcca
      481 gcgtgtacgc ttggaacagg aagagaatca gcaactgcgt ggccgactac tctgtgctgt
      541 acaactctgc ctccttctcc accttcaagt gctacggcgt gtccccaacc aagctgaacg
      601 acctgtgctt caccaacgtg tacgctgact ccttcgtgat cagaggagac gaagtgagac
      661 agatcgctcc cggtcagacc ggaaagatcg ccgactacaa ctacaagctg ccagacgact
      721 tcaccggttg cgtgatcgct tggaactcta acaacctgga ctctaaggtg ggaggcaact
      781 acaactacct gtacaggctg ttccgcaaga gcaacctgaa gcctttcgaa agggacatca
      841 gcaccgaaat ctaccaggct ggatctaccc catgcaacgg agtggaagga ttcaactgct
      901 acttccctct gcagtcttac ggattccagc caaccaacgg agtgggatac cagccataca
      961 gagtggtggt gctgtctttc gagctgctgc acgctcctgc taccgtgtgc ggaccaaaga
     1021 agtctaccaa cctggtgaag aacaagtgcg tgaacttcaa gcacccagct ttcttgtaca
     1081 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     1141 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     1201 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     1261 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     1321 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     1381 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     1441 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     1501 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     1561 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     1621 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     1681 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     1741 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     1801 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     1861 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     1921 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     1981 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     2041 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     2101 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     2161 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     2221 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2281 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2341 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2401 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2461 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2521 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2581 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2641 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2701 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2761 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2821 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2881 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2941 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3001 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcgg
//
