LOCUS       sequence-32948-e         556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, 
            Martin-Belmonte F, Mostov KE
  TITLE     A molecular network for de novo generation of the apical surface and
            lumen.
  JOURNAL   Nat Cell Biol. 2010 Nov;12(11):1035-45. doi: 10.1038/ncb2106. Epub 
            2010 Oct 3.
  PUBMED    20890297
REFERENCE   2  (bases 1 to 556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2106"; 
            journalName: "Nat Cell Biol"; date: "2010-11"; volume: "12"; issue: 
            "11"; pages: "1035-45"
COMMENT     Sequence Label: pLKO.1-puro-cfRab8a_2
FEATURES             Location/Qualifiers
     source          1..556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     107..126
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    279..396
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tggaattaat ttgactgtgt acacaaagat attaatacaa ggtacgtgac ggggaangta
       61 gtaatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
      121 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
      181 caccggggcc ctccccctcc aatactctcg agagtattgg agggggaggg cctttttgaa
      241 ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
      301 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
      361 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
      421 gatccacttt ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg
      481 gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcaccg gcgccgaccc
      541 tgggtctcgc acattc
//
