LOCUS       sequence-329484-       11069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of IQSEC1 splice variant 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, 
            Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L,
            Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth
            K, Bryant DM
  TITLE     An ARF GTPase module promoting invasion and metastasis through 
            regulating phosphoinositide metabolism.
  JOURNAL   Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
  PUBMED    33712589
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-12"; volume: "12(1):1623. doi"; pages: " 
            10.1038/s41467-021-21847-4"
COMMENT     Sequence Label: pLEX304-GFP-v3 IQSEC1 WT
FEATURES             Location/Qualifiers
     source          1..11069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    22..255
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             440..484
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        659..1159
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1078..1096
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1171..1566
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    1626..1743
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1860..2163
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2164..2367
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2318..2338
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2406..2415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2412..2414,2415..2417,2418..3128)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2412..2414,2415..2417,2418..3128)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2457..2478)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2718..2737)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3065..3086
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5871..6459
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5924..5944)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6342..6353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6531..6764
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6842..6963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6879..6898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6933..6952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7003..7138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7158..7177)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7159..7177)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7187..7204)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7187..7203)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7196..7218)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7345..7800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7432..7451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7642..7663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7826..7930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7931..7999,8000..8791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8149..8168)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8962..9550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9451..9470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9704..9721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9838..9859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9874..9879,9880..9897,9898..9904)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9912..9928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9917..9939
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9936..9952
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9936..9952
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9971..9991
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9973..9991
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10019..10245
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10246..10426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10473..10598
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
       61 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
      121 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
      181 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
      241 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
      301 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
      361 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
      421 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
      481 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
      541 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
      601 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggaccctt
      661 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
      721 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
      781 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
      841 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
      901 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
      961 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     1021 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     1081 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     1141 caccgacctc tctccccagg gggtaccacc atggccaagc ctttgtctca agaagaatcc
     1201 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
     1261 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
     1321 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
     1381 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
     1441 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
     1501 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
     1561 gagggcctgc agctgcagta gtaagaattc tagatcttga gacaaatggc agtattcatc
     1621 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     1681 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     1741 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gggggcccgg
     1801 ggttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1861 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1921 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1981 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2041 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2101 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2161 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2221 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2281 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2341 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
     2401 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2461 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     2521 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2581 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     2641 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2701 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2761 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     2821 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     2881 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     2941 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3001 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     3061 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     3121 tgtacaagtc cggactcaga tctcgagctc aagcttacat gctagagcga aagtatgggg
     3181 ggcgcctggt aacccgccat gcggcccgca ccatccagac ggcgtttcgc caatatcaaa
     3241 tgaataaaaa cttcgagcgc ttgcgcagct ccatgtcaga gaaccgcatg tcacgccgga
     3301 ttgtgctgtc caacatgagg atgcagttct cctttgaggg gcctgagaaa gtgcacagct
     3361 cctacttcga ggggaagcag gtctcagtga ctaacgacgg ctcccagctg ggagccctgg
     3421 tgtcccctga gtgtggtgac ctcagcgagc ccaccaccct caagtctccg gccccctcca
     3481 gtgactttgc ggacgccatc accgagctgg aggacgcctt ctctaggcaa gtgaaatcac
     3541 tggccgagtc catcgacgat gccctcaact gccgcagcct gcacactgag gaggcaccgg
     3601 ccctggatgc ggcgcgggcc cgggacaccg aaccccagac agccctgcac ggcatggacc
     3661 accgcaaact ggacgagatg acggcctcgt acagtgatgt caccctgtac atcgatgagg
     3721 aggagctgtc gccccctctg cccctctcgc aggcagggga ccggccgtcc agcaccgagt
     3781 cggacctgcg gctacgggct gggggcgcag ccccagacta ctgggccctg gcccacaaag
     3841 aggacaaggc tgacacggac acgagctgcc ggagcacgcc gtcgctggag cggcaggagc
     3901 agcggctgcg ggtggagcat ctgccgctgc tcaccatcga gccacccagc gacagctctg
     3961 tggaccttag tgaccgctcg gagcgggggt cactcaagag gcagagtgct tacgagcgca
     4021 gccttggcgg gcagcagggc agtcccaagc atggtcccca cagcggcgcc cccaagagcc
     4081 tcccccggga ggagcctgag ttgcggcccc ggccccccag gcccctggac agccacttgg
     4141 ccatcaatgg ctcagccaac cggcagagca agtctgagtc ggactactca gacggtgaca
     4201 atgacagcat caacagcacg tccaactcca acgataccat caactgcagc tccgagtcat
     4261 cgtcccgtga cagcctgcgg gagcagacgc tcagcaagca gacctaccac aaggaggccc
     4321 gcaacagctg ggactcgcct gcctttagca acgatgtcat ccgcaagagg cactaccgca
     4381 tcggcctgaa cctcttcaac aagaagcctg agaagggagt ccagtacctc atcgagcgtg
     4441 gctttgtgcc cgacacgccc gtcggggtgg cccacttcct gctgcagcgc aagggcctca
     4501 gccggcagat gatcggcgag ttcctgggca accggcagaa gcagttcaac cgtgacgtgc
     4561 tcgactgcgt cgtggacgag atggacttct ctaccatgga gctggatgag gccctcagga
     4621 aattccaggc gcacatccgt gtccaagggg aggctcagaa agtggagcgg ctcatagagg
     4681 cgttcagcca gcgctactgc atctgcaacc ctggggtggt gcggcaattc cggaacccag
     4741 acaccatttt catcctggcc ttcgccatca tcctgctgaa caccgacatg tacagcccca
     4801 atgtcaagcc cgagcggaaa atgaagctag aggacttcat caagaacctc cgaggtgtgg
     4861 acgatggtga ggacattccc cgtgagatgc tgatggggat ctatgaacgg atccgtaagc
     4921 gagagctaaa gaccaatgag gaccatgtgt cccaggtgca gaaggtggag aagctcattg
     4981 tggggaaaaa gccgatcgga tccctgcatc ccgggctcgg ctgtgtgctc tctctgcccc
     5041 accgtcggtt ggtctgctac tgccggctct ttgaggttcc agacccaaac aagccccaga
     5101 aactcggact acaccagcga gaaatcttcc tgttcaacga cctcctggtg gtcaccaaga
     5161 tcttccagaa gaagaagaac tcggtgacgt acagcttccg acagtccttc tccttgtacg
     5221 gcatgcaggt cctgctcttc gagaaccagt actaccccaa tggcatccgg ctcacctcgt
     5281 ctgtccccgg agcagatatc aaagtgttaa taaacttcaa cgcccccaac cctcaagacc
     5341 ggaagaaatt caccgatgac ctgcgggagt ccattgcgga agtccaagag atggagaagc
     5401 acaggataga gtcggagctc gagaagcaga aaggcgtcgt gcggcccagc atgtcccagt
     5461 gctctagcct caaaaaggag tcgggcaacg gaacactgag ccgggcctgc ctggacgaca
     5521 gctatgccag cggtgagggc ctcaagcgca gcgccctcag cagctccctg cgggacctct
     5581 cggaagccgg gaagcgaggg cgtcgcagca gtgcgggatc gctagagagc aatgtggaat
     5641 ttcagccttt cgagccactg cagccgtcag tgctgtgctc ctaagaattc tgcagtcgac
     5701 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca
     5761 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     5821 aaaatgaatg caattgttgt tgttgctaac cggtggcgcg ttaagtcgac aatcaacctc
     5881 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5941 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6001 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6061 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6121 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6181 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6241 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6301 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6361 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6421 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     6481 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6541 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     6781 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6841 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6901 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6961 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     7021 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7081 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7141 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     7201 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     7261 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     7321 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7381 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7441 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7501 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7561 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7621 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7681 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7741 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     7801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7861 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7921 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7981 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8041 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8101 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8161 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8221 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8281 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8341 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8401 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8461 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8521 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8581 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8641 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8701 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8761 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9601 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9661 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9721 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9781 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9841 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9901 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     9961 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
    10021 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
    10081 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
    10141 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
    10201 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
    10261 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    10321 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    10381 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
    10441 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
    10501 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
    10561 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
    10621 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
    10681 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
    10741 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
    10801 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
    10861 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
    10921 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
    10981 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
    11041 accaaggcaa agagaagagt ggtgcagag
//
