LOCUS       sequence-329495-       10993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of Flag-Polß(TM: L301R/V303R/V306R) protein 
            with hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, 
            Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW
  TITLE     HSP90 regulates DNA repair via the interaction between XRCC1 and DNA
            polymerase beta.
  JOURNAL   Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513.
  PUBMED    25423885
REFERENCE   2  (bases 1 to 10993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-26"; volume: "5:5513. doi"; pages: " 
            10.1038/ncomms6513"
COMMENT     Sequence Label: pLVX-Flag-Polß(TM)-IRES-hyg
FEATURES             Location/Qualifiers
     source          1..10993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     76..93
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    292..316
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1344..1367)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(1391..1415)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1510..1534)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1531..1734)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1560..1580)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1735..2038
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    2096..2211
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(2474..2518)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2703..2936
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3433..3558
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3605..4238
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4406..4540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4430..4449)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4484..4503
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4588..4692
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6031..6099,6100..6891)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6249..6268)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7062..7650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7551..7570
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7804..7821
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7938..7959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7974..7979,7980..7997,7998..8004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8012..8028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8017..8039
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8036..8052
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8036..8052
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             8180..8813
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9020..9608
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9126..9137
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9535..9555
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9622..10641)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             complement(9622..10641)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    join(10667..10675,10676..10678,10679..10993,1..260)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10840..10859)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct tccttcacga
       61 cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga atgctcgtca
      121 agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat atggtggaaa
      181 ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca gtaacgttag
      241 ggggggggga gggagagggg cgggatccgc ggccgctcta gaactagatc aaccactttg
      301 tacaagaaag ctgggtcggc gcgcccaccc tttcattcgc tccggtcctt gggttcccgg
      361 tatttccact ggatgtaatc aaagatgtct ttttcactat ccactggcag gggttctcct
      421 gcacgtccag tgcgtccccg gggacggatg gtgtactcat tgattgtgaa acccttttct
      481 agggcatgag ccctcatatt cttattgaaa atatcactcc cagtgaaata gagaacacca
      541 cagtaatact gatctttggg tatcaacctg atatcaattc ttctgtgtgg atattctttt
      601 tcatcatttt tactgggaag ctggcaaaca cccatgaact ttgtctcacc ctttgacagg
      661 gtatctgtga taaaatgaac cttttgtaac tgctccacaa cctgatgtaa cagttttggc
      721 tgtttggttg attctgaagt gaagctggga tgggtcagga gaacatccat gtcaccactg
      781 gactctgcac ctcttctgaa actgccacag actgtagcaa tgtattcaga atccactttt
      841 ttaacttcat ttagtacaat atcttgcatt tgtaacatct cttcacgagg aattcttttt
      901 tcaaagtccc caaaatattt cagcccaatt cgctgatgat ggttcaattt atcttcattt
      961 tttctgagat cttctagtgt tttaattcct tcatctacaa acttccttgc agcagatgga
     1021 ccaatgccac taactcgagt caggaaattg atggatgaac tcgtatcatc ctgccgaatc
     1081 ttttccagtt tacgtaattt tccagttgct aaaaactcat caatcttttc agcaattttt
     1141 gttcctactc caggcaattt cttagcttca gctccactct ttattttgtg tgggtatttt
     1201 gctataacag atgctgcttt tctgtaagca ttgtacttgt ggatagcttg gctcacgttc
     1261 ttctcaaagt ttgcgagttc tgtgagcatg tcggtgattc ccccgttgag agtctcctgc
     1321 ggcgccttcc gtttgctcat gcctttatcg tcatcgtctt tgtagtccat ggtgaagggg
     1381 gcggccgcgg agcctgcttt tttgtacaaa cttgttgatc tagtctcgag gaattcaccg
     1441 gaaatagatc ctctagtaga gtcggtgtct tctatggagg tcaaaacagc gtggatggcg
     1501 tctccaggcg atctgacggt tcactaaacg agctctgctt atatagacct cccaccgtac
     1561 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
     1621 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
     1681 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
     1741 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
     1801 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
     1861 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
     1921 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
     1981 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
     2041 gaactcccaa gcttatcgat aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt
     2101 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
     2161 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aggcgatacc
     2221 gtcgagatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt
     2281 ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg
     2341 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
     2401 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
     2461 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
     2521 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
     2581 ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc
     2641 aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc
     2701 ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct
     2761 tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat
     2821 tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg
     2881 acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc
     2941 ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta
     3001 atggttcaat ttttactact ttatatttat ataattcact tctccaattg tccctcatat
     3061 ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt
     3121 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     3181 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     3241 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     3301 gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     3361 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     3421 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     3481 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     3541 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     3601 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     3661 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     3721 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     3781 gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc
     3841 agtcccgccc aggccacgcc tccctggaaa gtccccagcg gaaagtccct tgtagcaagc
     3901 tcgatatcag cagttcttga agtactccgg atgcagctct cgggccacgt gatgaaatgc
     3961 taggcggctg tcaaacctcc actctaacac ttctctctcc gggtcatcca tcccatgcag
     4021 gctcacaggg tgtaacaagc tggtgttctc tcctttattg gcctcttcta ccttatctgg
     4081 ctcaactggt actagcttgt agcaccatcc aaaggtcagt ggatatctga cccctggccc
     4141 tggtgtgtag ttctgctaat cagggaagta gccttgtgtg tggtagatcc acagatcaag
     4201 gatatcttgt cttctttggg agtgaattag cccttccaac tactaagttt gtagtacata
     4261 tttaacaaat acaatttctt taaaatgaaa ataattcaga ggaatcacag gtttagagta
     4321 aatgaaacca caggtaattg gcagtggtaa tagggtatgg ggtgggaagt ttgggatgat
     4381 tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg atgagtttgg
     4441 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     4501 tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga tccgtcgacg
     4561 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4621 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4681 aaaaggaaga gtatgagtac tgagagatcc cctcataatt tccccaaagc gtaaccatgt
     4741 gtgaataaat tttgagctag tagggttgca gccacgagta agtcttccct tgttattgtg
     4801 tagccagaat gccgcaaaac ttccatgcct aagcgaactg ttgagagtac gtttcgattt
     4861 ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg aacgcccgca
     4921 agccaacatg ttagttgaag catcagggcg attagcagca tgatatcaaa acgctctgag
     4981 ctgctcgttc ggctatggcg taggcctagt ccgtaggcag gacttttcaa gtctcggaag
     5041 gtttcttcaa tctgcattcg cttcgaatag atattaacaa gttgtttggg tgttcgaatt
     5101 tcaacaggta agttagttgc tagaacccat ggctcctttg ccgacgctga gtagatttta
     5161 ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc tttagagcga
     5221 gatttataca atagaatttg gcatgagatt ggattgcttt tagtcagcct cttatagcct
     5281 aaagtctttg agtgactaga tgacatatca tgtaagttgc tgataggttt ccagttttcc
     5341 gctcctaggt ctgcatattg tacttttcct cttactcgac ttaaccagta ccaacccagc
     5401 ttctcaacgg atttatacca tggcacttta aagccagcat cactgacaat gagcggtgtg
     5461 gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc tttctttgaa
     5521 cattgctctg aaagcgggaa cgctttctca taaagagtaa cagaacgacc gtgtagtgcg
     5581 actgaagctc gcaataccat aagtcgtttt tgctcacgaa tatcagacca gtcaacaagt
     5641 acaatgggca tcgtattgcc cgaacagata aagctagcat gccaacggta tacagcgagt
     5701 cgctctttgt ggaggtgacg attacctaac aatcggtcga ttcgtttgat gttatgtttt
     5761 gttctcgctt tggttggcag gttacggcca agttcggtaa gagtgagagt tttacagtca
     5821 agtaatgcgt ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa ttcggggcag
     5881 aattggtaaa gagagtcgtg taaaatatcg agttcgcaca tcttgttgtc tgattattga
     5941 tttttcgcga aaccatttga tcatatgaca agatgtgtat ccaccttaac ttaatgattt
     6001 ttaccaaaat cattagggga ttcatcaggt atgagtattc aacatttccg tgtcgccctt
     6061 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6121 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6181 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6241 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6301 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6361 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6421 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6481 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6541 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6601 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6661 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6721 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6781 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6841 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6901 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6961 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7021 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7081 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7141 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7201 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7261 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7321 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7381 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7441 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7501 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7561 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7621 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7681 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7741 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7801 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7861 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7921 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7981 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8041 aacagctatg accatgatta cgccaagctc tagctagagg tcgacggtaa acgctagcaa
     8101 tgtcaaggcc tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata
     8161 agatgacatg aactactact gctagagatt ttccacactg actaaaaggg tctgagggat
     8221 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     8281 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     8341 tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag
     8401 ggctcgccac tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag
     8461 tcccttgtag caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc
     8521 cacgtgatga aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc
     8581 atccatccca tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc
     8641 ttctacctta tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata
     8701 tctgacccct ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta
     8761 gatccacaga tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct
     8821 cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg
     8881 tacctgaggt gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca
     8941 atcagcattg gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg
     9001 actgcagaat taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg
     9061 cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc
     9121 gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca
     9181 acacaggcga gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca
     9241 gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc
     9301 gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg
     9361 ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca
     9421 acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg
     9481 aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc
     9541 gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga
     9601 ggttgattgt tccagacgcg tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt
     9661 tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc
     9721 gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg
     9781 cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc
     9841 aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc
     9901 gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc
     9961 gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg
    10021 gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc
    10081 ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac
    10141 ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa
    10201 atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt
    10261 ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc
    10321 gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca
    10381 ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc
    10441 cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac
    10501 ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc
    10561 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
    10621 cgtcgcggtg agttcaggct ttccggatct atccatcttt ggtacgggtt gtggcaagct
    10681 tatcatcgtg tttttcaaag gaaaaccacg tccccgtggt tcggggggcc tagacgtttt
    10741 tttaacctcg actaaacaca tgtaaagcat gtgcaccgag gccccagatc agatcccata
    10801 caatggggta ccttctgggc atccttcagc cccttgttga atacgcttga ggagagccat
    10861 ttgactcttt ccacaactat ccaactcaca acgtggcact ggggttgtgc cgcctttgca
    10921 ggtgtatctt atacacgtgg cttttggccg cagaggcacc tgtcgccagg tggggggttc
    10981 cgctgcctgc aaa
//
