LOCUS       sequence-329496-        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of Flag-Polß(L301R/V303R) protein with 
            puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, 
            Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW
  TITLE     HSP90 regulates DNA repair via the interaction between XRCC1 and DNA
            polymerase beta.
  JOURNAL   Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513.
  PUBMED    25423885
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-26"; volume: "5:5513. doi"; pages: " 
            10.1038/ncomms6513"
COMMENT     Sequence Label: pLVX-Flag-Polß(L301R/V303R)-IRES-puro
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1014..1148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1105..1124
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1316..1949
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1996..2121
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2618..2851
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3036..3080
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3343..3458
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        3516..3819
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3820..4023
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3974..3994
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4020..4044
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4139..4163
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4187..4210
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(5238..5262)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5294..5868,5869..5871,5872..5880)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5461..5478)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5688..5707
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5900..6499
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5900..5919)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6396..6416
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6513..7101
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6566..6586)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6984..6995)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7308..7941
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8069..8085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8069..8085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8082..8104)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8093..8109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8117..8123,8124..8141,8142..8147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8162..8183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8300..8317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8471..9059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8551..8570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt
     1021 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1081 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1141 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     1201 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
     1261 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa
     1321 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
     1381 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     1441 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     1501 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     1561 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     1621 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     1681 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     1741 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     1801 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     1861 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1921 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     1981 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     2041 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     2101 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     2161 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     2221 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     2281 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     2341 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     2401 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     2461 gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     2521 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     2581 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     2641 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     2701 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     2761 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     2821 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     2881 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     2941 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     3001 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     3061 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     3121 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     3181 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     3241 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     3301 acagatccat tcgattagtg aacggatctc gacggtatcg cctttaaaag aaaagggggg
     3361 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     3421 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     3481 agagatccag tttatcgata agcttgggag ttccgcgtta cataacttac ggtaaatggc
     3541 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     3601 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     3661 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     3721 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     3781 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     3841 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3901 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3961 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     4021 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     4081 agaagacacc gactctacta gaggatctat ttccggtgaa ttcctcgaga ctagatcaac
     4141 aagtttgtac aaaaaagcag gctccgcggc cgcccccttc accatggact acaaagacga
     4201 tgacgataaa ggcatgagca aacggaaggc gccgcaggag actctcaacg ggggaatcac
     4261 cgacatgctc acagaactcg caaactttga gaagaacgtg agccaagcta tccacaagta
     4321 caatgcttac agaaaagcag catctgttat agcaaaatac ccacacaaaa taaagagtgg
     4381 agctgaagct aagaaattgc ctggagtagg aacaaaaatt gctgaaaaga ttgatgagtt
     4441 tttagcaact ggaaaattac gtaaactgga aaagattcgg caggatgata cgagttcatc
     4501 catcaatttc ctgactcgag ttagtggcat tggtccatct gctgcaagga agtttgtaga
     4561 tgaaggaatt aaaacactag aagatctcag aaaaaatgaa gataaattga accatcatca
     4621 gcgaattggg ctgaaatatt ttggggactt tgaaaaaaga attcctcgtg aagagatgtt
     4681 acaaatgcaa gatattgtac taaatgaagt taaaaaagtg gattctgaat acattgctac
     4741 agtctgtggc agtttcagaa gaggtgcaga gtccagtggt gacatggatg ttctcctgac
     4801 ccatcccagc ttcacttcag aatcaaccaa acagccaaaa ctgttacatc aggttgtgga
     4861 gcagttacaa aaggttcatt ttatcacaga taccctgtca aagggtgaga caaagttcat
     4921 gggtgtttgc cagcttccca gtaaaaatga tgaaaaagaa tatccacaca gaagaattga
     4981 tatcaggttg atacccaaag atcagtatta ctgtggtgtt ctctatttca ctgggagtga
     5041 tattttcaat aagaatatga gggctcatgc cctagaaaag ggtttcacaa tcaatgagta
     5101 caccatccgt cccttgggac gcactggagt tgcaggagaa cccctgccag tggatagtga
     5161 aaaagacatc tttgattaca tccagtggaa ataccgggaa cccaaggacc ggagcgaatg
     5221 aaagggtggg cgcgccgacc cagctttctt gtacaaagtg gttgatctag ttctagagcg
     5281 gccgcggatc ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     5341 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     5401 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     5461 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     5521 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     5581 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     5641 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     5701 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     5761 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     5821 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc
     5881 cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5941 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     6001 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     6061 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     6121 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     6181 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6241 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6301 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6361 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     6421 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     6481 ccgcaagccc ggtgcctaga cgcgtctgga acaatcaacc tctggattac aaaatttgtg
     6541 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6601 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6661 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6721 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6781 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6841 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6901 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6961 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7021 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7081 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg
     7141 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     7201 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     7261 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
     7321 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     7381 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     7441 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     7501 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     7561 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     7621 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     7681 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     7741 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7801 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7861 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7921 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7981 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga
     8041 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8101 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8161 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8221 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8281 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8341 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8401 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8461 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8521 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8581 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8641 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8701 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8761 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8821 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8881 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8941 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9001 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9061 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9121 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9181 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacag
//
