LOCUS       sequence-329497-        4596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri DDX3X.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-DDX3X
FEATURES             Location/Qualifiers
     source          1..4596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2721..2820)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2837..2856)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2838..2856)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2861..2877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2861..2877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2979..2998)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2990..3799
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3057..3076)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3946..4534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4435..4454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgag tcatgtggtc gttgatactc ctcacggtct
      721 agatcagcag cttgctgtcc tagacttgaa ctccgctgac ggacagggtg gaggaactgg
      781 caggcgatac attccacctc acctgaggaa caaagaggct tcaaaaaacg attcaccagg
      841 atgggataac ggacgcacca atggatttgt gaatggattc catgacaacc gcacaaacgg
      901 aggctttgga gggcggtggc cccttcgcaa cgatagaggt tttggtggtt ttgaaaacaa
      961 agatggtggc tggggagggc cccctagaga tgctgcctac aacagctttg gtgggcgtaa
     1021 tgatagatcc aaatctgctt ttaatgaccg tggggcgggc tccagaggaa ggtatgagcg
     1081 ggggggcttt gctggtggag gaaacagccg ttgggtggag gactccagag aggacgactg
     1141 gtctaaacca actgctccta atgaacgact ggaaaatgag ctcttttctg gaagcaacac
     1201 tgggatcaac tttgagaagt atgacgacat tcctgtggaa gctactggaa gcaactgccc
     1261 accccacatt gagagtttcc atgatgtgga catgggggag atcatcatgg gcaacatcac
     1321 cctgactcgt tacacccgtc ccactccagt ccagaaacat gctataccca tcatcaagtc
     1381 caagagggac ctgatggctt gtgcccaaac aggttctggt aagactgcag ccttcctgct
     1441 gccagtgctg agtcagatct acacagatgg accaggagat gctctgcagg ctgcaaaaaa
     1501 cagtggacag gacaatggaa gatatggccg gcgtaagcag taccccatct ccctggtcct
     1561 ggcccccacc agagaattgg ctctgcagat ctacgacgag gcgaggaagt ttgcgtaccg
     1621 ctctcgtgtg cgtccctgtg tggtctatgg aggagctgat attggccagc aaatccggga
     1681 cttggaaaga ggctgtcact tgttggtggc cacacctgga cgtctggttg acatgatgga
     1741 gaggggcaag attggtctgg accactgcaa cttcctggtc ctggatgagg ctgaccgtat
     1801 gttggacatg ggctttgaac cacaaatcag acgcatcgtg gagcaggaca cgatgccacc
     1861 aaaaggtatc cgtcagacca tgatgttcag tgccacgttc cctaaagaga ttcagattct
     1921 ggctcgagac ttcctggagg attacatttt cctggcagta ggccgtgttg gttccacttc
     1981 agaaaacatc acccagaagg tggtgtgggt ggaggagaac gacaagaggt cctttcttct
     2041 ggacttgctt aatgccacag gtaaagactc attgacccta gtctttgtgg aaacgaagaa
     2101 aggagcagat gctctggagg acttcctgta ccgtgagggt tacgcctgca ccagtatcca
     2161 tggagacaga tcccagagag accgagaaga agctctgcat cagtttcgct ctggacgctg
     2221 cccgatctta gtggctacag ctgtggctgc tcgaggtctg gacatcagca acgtcaaaca
     2281 cgttattaat tttgatttgc ccagtgacat tgaagagtac gttcaccgta tcggccgtac
     2341 ggggcgtgtg ggcaatctag gtctggccac gtcgttcttc aacgacaaaa acagcaacat
     2401 aaccaaagat ttgctggaca tcttggttga ggccaagcag gaggttcctt cctggcttga
     2461 gagcctggcc tacgagcacc agcacaagag caccaaccga ggacgctcca aaaggttctc
     2521 gggtggtttt ggagccaggg actaccgtca ggcacctggt ggttctggag gcttcagcag
     2581 caaccgtgta gggcgaggtg ctggaggcca cggaggaaat cgtggctttg gtggaggtgg
     2641 ttttggtggc aacttctaca gcaacgatgg ctacggcgga aactacagcc actctggtag
     2701 tgtggattgg tggggaaacg acccagcttt cttgtacaaa gttggcatta taagaaagca
     2761 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2821 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2881 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2941 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     3001 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     3061 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     3121 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     3181 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     3241 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     3301 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     3361 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     3421 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     3481 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     3541 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     3601 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     3661 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     3721 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     3781 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     3841 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     3901 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3961 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4021 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4081 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4141 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4201 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4261 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4321 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4381 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4441 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4501 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4561 gttcctggcc ttttgctggc cttttgctca catgtt
//
